Dietary quenching enzyme AiiO-AIO6 promotes fish growth through intestinal microbiota: Zebrafish as a model

Supplemental N-acyl homoserine lactonase alleviates intestinal disruption and improves gut microbiota in broilers challenged by Salmonella Typhimurium

“…These findings suggested that the reductions in these harmful bacteria together with increases in the above-mentioned beneficial bacteria could contribute to the observed reversing effect of AHLase on intestinal disruption and growth retardation in S. Typhimurium-challenged broilers. Similarly, previous studies in aquatic animals revealed that supplemental AHLase or AHL-degrading probiotics promoted the growth and intestinal health by improving gut microbial composition, characterized by the increases and decreases in certain beneficial and harmful bacteria, respectively [ 16 , 21 ].…”

“…Consistent with previous studies [ 1 , 2 ], we observed the adverse impacts of S. Typhimurium challenge on broiler growth performance, as exhibited by the reductions of BW on both d 10 and 14 together with ADG during d 1–10 and d 1–14. Treatment with AHLase or AHL-degrading probiotics was reported to improve growth performance of aquatic animals [ 16 , 21 ]. Likewise, the present study confirmed an alleviation of S. Typhimurium-induced reductions of BW and ADG in response to AHLase addition especially at a dosage of 10 U/g.…”

“…In concert with previous studies [ 1 , 2 ], we found that S. Typhimurium challenge impaired intestinal morphology of broilers on d 10, which could correspond to the synchronous increase in serum DAO activity in challenged birds. Previously, a study in zebrafish revealed that AHLase addition improved intestinal morphology, as characterized by increases in VH and goblet cell density [ 16 ]. In this study, we noted that AHLase addition counteracted the compromise of intestinal morphology including the elevated CD and reduced V/C of jejunum and ileum in challenged birds.…”

“…As a result, AHLase treatment represents a promising candidate for use as an approach against S. Typhimurium infection in animals. Although there were studies regarding the positive effects of AHLase on growth, survival and intestinal absorption in aquatic animals [14][15][16], almost no study was available regarding the application of AHLase in poultry production. Accordingly, the present study was conducted to probe if AHLase addition could attenuate S. Typhimurium-induced detriments to immunity, intestinal health and growth performance of broilers, aiming to provide a new strategy to limit Salmonella infection in chicken production.…”

Quorum-quenching potential of recombinant PvdQ-engineered bacteria for biofilm formation

“…These QQ bacteria achieve QS inhibition by producing enzymes with AHL degradation activity and reducing AHL signal concentrations (Bzdrenga et al 2017;Ghanei-Motlagh et al, 2021). Interestingly, the QQ enzyme does not kill the pathogens, however it reduces the concentration of signal molecules (Lin et al 2003;Yao et al 2022).…”