Dietary Pterostilbene and Resveratrol Modulate the Gut Microbiota Influenced by Circadian Rhythm Dysregulation

Abstract

Scope A causal relationship between circadian misalignment and microbiota dysbiosis has been discussed recently, due to their association to pathogenesis. Herein, the possible impact of pterostilbene (PSB) and resveratrol (RES) on the gut microbiota brought by chronic jet‐lag in mice is investigated. Methods and Results Dietary supplement of RES and PSB (0.25%) are given to 16 week‐jetlagged mice to examine the effects on microbiota and physiological functions. Jetlag significantly induces weight gained that could be effectively prevented by PSB. Both supplements also retain oscillation patterns that found to be lost in jetlag induced (JLG) group, including serum biochemical parameters and gut microbiota. The results of beta diversity suggest the supplementations efficiently lead to distinct gut microbial composition as compared to JLG group. Besides, the supplementation forestalls some microbial elevation, such as Eubacterium ventriosum and Acetitomaculum. Growth of health beneficial bacteria like Blautia and Lachnospiraceae UCG‐001 is facilitated and abundance of these bacteria could be correlated to oscillation of biochemical parameters. Result of KEGG indicates distinct effect brought by microbial re‐shaping. Conclusion The result suggests that supplementation of RES and PSB could potentially dampen some adverse effects of gut microbiota dysbiosis, and at the same time, re‐composite and facilitate the growth of health beneficial microbiota.

Modulatory Effect of Fermented Black Soybean and Adlay on Gut Microbiota Contributes to Healthy Aging

“…Sequencing analysis was performed as previously described. [59] Statistical Analysis: A student t-test was performed to indicate significant differences between groups. The sample size of each group ranged between four and six mice (or three mice for gut microbial analysis groups).…”

Piceatannol and 3′-Hydroxypterostilbene Alleviate Inflammatory Bowel Disease by Maintaining Intestinal Epithelial Integrity and Regulating Gut Microbiota in Mice

“…Gut microbiota analysis was performed according to the method described previously. 23 Isolating and purifying genomic DNA of the gut bacteria using an InnuPREP Stool DNA kit with slight modifications, the DNA samples were then sent to Biotools Co. Ltd for analysis of the fecal microbial composition via the 16S amplicon sequencing technique. PCR was used to amplify the 16S rRNA gene from each DNA sample, including 10 conserved regions (V3−V4) and 9 hypervariable regions (V1− V9).…”

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“…Similar results have been obtained by Koh et al., who treated jetlagged mice with pterostilbene and resveratrol. In these animals, the combination of pterostilbene and resveratrol improved gut diversity ( 246 ).…”