Dietary probiotic inclusion level modulates intestinal mucin composition and mucosal morphology in broilers

Tsirtsikos, P.; Fegeros, K.; Balaskas, Christos; Kominakis, A.; Mountzouris, Konstantinos C.

doi:10.3382/ps.2011-02005

Cited by 65 publications

(61 citation statements)

References 44 publications

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“…Awad et al (10) found no difference in duodenal villus height and width after synbiotic supply. The same result of no difference after probiotic supply within duodenal villus height and crypt depth was reported by Tsirtsikos et al (26). Pelicano et al (8) proved higher crypt depth in the duodenum after Bacillus subtilis addition compared to the control group but there was no difference in villus height.…”

Section: Discussionsupporting

confidence: 81%

Effect of Effective Microorganisms on intestinal morphology and morphometry in Japanese quails

Gesek¹,

Sokół²,

Lambert³

et al. 2018

Turk J Vet Anim Sci

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The aim of this study was to determine the effect of supplementation with Effective Microorganisms (EM) on the morphology and the morphometry of the intestine, and on proliferation activity of crypt cells in Japanese quails. The EM group (16 birds) received EM for 30 days in water (3 L/1000 L of water) and in feed (5 kg/1000 kg of feed) mixed with standard diet. The second group (16 birds) was established as the control group (no EM supplementation). Histopathological analysis revealed infiltration of lymphoid cells mainly in the duodenum and jejunum similarly in the EM and the control groups. The EM group showed irregular villus surfaces when compared to the control group. Morphometric analysis revealed significant differences in the EM group for villus width in the duodenum (186.0 µm to 170.7 µm; P ≤ 0.05), in crypt depth (129.2 µm to 110.7 µm; P ≤ 0.05), muscular layer thickness in the jejunum (70.88 µm to 57.44 µm) (P ≤ 0.05), and muscular layer thickness in the ileum (76.88 µm to 64.76 µm; P ≤ 0.05). Immunohistochemical analysis of proliferating cell nuclear antigen activity was similar for the EM and the control groups: in the duodenum 90.82% in the control group and 91.89% in the EM group, in the jejunum 93.32% in the control group and 94.53% in the EM group, and in the ileum 92.53% in the control group and 93.92% in the EM group. Supplementation with EM resulted in improved structure and functions of the villi and changed their surface, height, width, and crypt depth.

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Section: Discussionsupporting

confidence: 81%

Effect of Effective Microorganisms on intestinal morphology and morphometry in Japanese quails

Gesek¹,

Sokół²,

Lambert³

et al. 2018

Turk J Vet Anim Sci

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“…Mannose and N-acetylglucosamine decreased linearly while fucose increased linearly with increasing probiotic treatment. 25 The microbial communities also contribute significantly in host nutrient metabolism providing folate, vitamin K and SCFAs. 26,27 A major function of gut microbiota is to break down non-digestible carbohydrates into SCFAs, particularly acetate, propionate and butyrate that serves as an important energy source for colonocytes.…”

Section: Mucus and Microbiotamentioning

confidence: 99%

Roles and regulation of the mucus barrier in the gut

2015

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“…Fuller (1989) concluded that probiotics are suitable alternatives for antibiotics as the latter have residues in meat, increase the resistant bacteria and resulted in imbalance of normal microflora. The action modes of probiotics, which affect gut function and health in poultry, include maintaining a normal micro-flora and beneficial microbial population by competitive exclusion and antagonism (Fuller, 1989), and improving gut mucin composition and amount (Tsirtsikos et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Effects of in Ovo Infusion of Probiotic Strains on Performance Parameters, Jejunal Bacterial Population and Mucin Gene Expression in Broiler Chicken

Majidi-Mosleh

Sadeghi

Mousavi

et al. 2017

Rev. Bras. Cienc. Avic.

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The objective of the present study was to evaluate the effects of in ovo infusion of probiotic strains (Bacillus subtilis, Enterococcus faecium, and Pediococcus acidilactici) on jejunal microbial population and mucin gene expression in broiler chicken. In a completely randomized design, 0.5 ml of mediums containing 10 7 cfu of different probiotic strains, was administered into amniotic fluid of the 480 Cobb fetus (day 18 of incubation), with four treatments, five replicates with twenty four eggs each. For mucin gene expression, samples from the jejunum were taken on day 21 of incubation and day 3 post-hatch. Microbial profile was determined for total lactobacillus and E. coli by sampling jejunal contents on days 1 and 3 of age. Expression of the mucin gene in the jejunum was higher (p<0.05) in chicks that received Bacillus subtilis in comparison with the control group. Infusion of the probiotic strains had no effect on jejunal E. coli and lactic acid bacteria populations on day 1 post-hatch (p>0.05). There were no significant differences among treatments for performance parameters at different periods and the whole period. It was concluded that infusion of probiotic bacteria during the late of incubation has no effect on feed intake, gain and feed conversion ratio, but has a positive effect on mucin gene expression in the jejunum. The best probiotic strain for mucin gene expression was Bacillus subtilis and for beneficiary bacteria colonization was Bacillus subtilis and Pediococcus acidilactici.

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Dietary probiotic inclusion level modulates intestinal mucin composition and mucosal morphology in broilers

Cited by 65 publications

References 44 publications

Effect of Effective Microorganisms on intestinal morphology and morphometry in Japanese quails

Effect of Effective Microorganisms on intestinal morphology and morphometry in Japanese quails

Roles and regulation of the mucus barrier in the gut

Effects of in Ovo Infusion of Probiotic Strains on Performance Parameters, Jejunal Bacterial Population and Mucin Gene Expression in Broiler Chicken

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