Dietary magnesium improves endothelial dependent relaxation of balloon injured arteries in rats

Fonseca, Francisco Antônio Helfenstein; Paiva, Therezinha B.; Silva, Eneida G.; Ihara, Sílvia Saiuli Miki; Kasinski, Nelson; Martinez, T.L.R.; Filho, Eulógio Emílio Martinez

doi:10.1016/s0021-9150(98)00069-0

Cited by 9 publications

(5 citation statements)

References 17 publications

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“…Phase 3 allows final repolarization of the cell by restoration of the membrane potential. Phase 4 is the stable phase with a resting potential of Ϯ90 mV (190) An important role of Mg 2ϩ in heart and vasculature function is a substantial vasodilatory effect that has been reported in animal and human studies (18,161,512,513). Although the results from animal studies suggest that Mg 2ϩ -induced vasodilation is due to the regulation of NO synthesis, human studies show that it is independent of NO activity (512,513).…”

Section: Serum Mgmentioning

confidence: 99%

Magnesium in Man: Implications for Health and Disease

Baaij

Hoenderop

Bindels

2015

Physiological Reviews

1,234

1,299

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Section: Serum Mgmentioning

confidence: 99%

Magnesium in Man: Implications for Health and Disease

Baaij

Hoenderop

Bindels

2015

Physiological Reviews

1,234

1,299

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show abstract

“…Male Sprague Dawley rats (weight, 246±12 g, n=28) were obtained from The Experimental Animal Center of Fujian Medical University (Fuzhou, China) and kept in a pathogen-free facility with a constant humidity (45%) and temperature (26°C) with a 12-h light/dark cycle and with free access to food and water. A rat model with vulnerable plaque was established as previously described ( 26 ). Briefly, following one week of feeding on standard rat chow, all rats were anesthetized with pentobarbital sodium (0.5 ml/100 g; 1% w/v in 0.9% saline) and underwent abdominal aorta balloon-injury (ABI) (n=20) or sham operation (n=8).…”

Section: Methodsmentioning

confidence: 99%

Ultrasound and magnetic resonance molecular imaging of atherosclerotic neovasculature with perfluorocarbon magnetic nanocapsules targeted against vascular endothelial growth factor receptor 2 in rats

Chen

Liang

et al. 2017

Molecular Medicine Reports

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The aim of the present study was to investigate the feasibility of using ultrasonography (US) and magnetic resonance (MR) for bimodal molecular imaging of atherosclerotic neovasculature with liquid perfluorocarbon magnetic nanocapsules (NCs) targeted to vascular endothelial growth factor receptor 2 (VEGFR-2). By incorporating perfluorooctyl bromide (PFOB) and superparamagnetic iron oxide (SPIO) into polylactic acid, a SPIO-embedded PFOB NC was constructed; subsequently, a VEGFR-2-targeted NC (VTNC) containing dual detectable probes was created by covalently linking a VEGFR-2 antibody onto the surface of the SPIO-embedded PFOB NC. Target specificity was verified in vitro by incubating VTNC with VEGFR-2+ or VEGFR-2− endothelial cells. Rats with vulnerable plaques were assigned to receive either an injection of VTNC (Targeted group; n=8) or an injection of NC (Nontargeted group; n=8); control rats also received an injection of VTNC (Control group; n=8). US and MR imaging of the abdominal aorta were performed to detect VTNC by measuring of the ultrasonic grayscale intensity (GSI) and MR contrast-to-noise ratio (CNR) prior to and at successive time points following VTNC and NC injections. The percent positive area (PPA) of CD31+ (PPACD31+) or VEGFR-2+ (PPAVEGFR-2+) expression was quantified by immunohistochemical staining. CD31 was used to verify the existence of endothelial cells as it is widely expressed on the surface of endothelial cells whether activated or not. The results demonstrated that VTNC was able to highly and selectively detect VEGFR-2+ endothelial cells, and GSI, CNR, PPACD31+ and PPAVEGFR-2+ were significantly increased in the targeted group compared with the nontargeted and control groups. In the control group, no atherosclerotic plaques or angiogenesis was identified, thus no expression of PPACD31+ and PPAVEGFR-2 (data not shown). There were strong correlations among GSI, CNR, PPACD31+ and PPAVEGFR-2+. In conclusion, two-probe VTNC is feasible for bimodal US and MR molecular imaging of atherosclerotic neovasculature, which may offer complementary information for the more reliable prediction of plaque vulnerability.

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“…One hypothesis is that EDNO is involved in magnesium induced vascular dilation. [14][15][16][17] The studies that support this hypothesis were all performed in animals. However, there have been no reports concerning magnesium induced coronary response and EDNO in humans.…”

Section: Does Edno Contribute To Magnesium Induced Human Coronary Dilation In Vivo?mentioning

confidence: 99%

“…12 13 One potential mechanism responsible for magnesium induced coronary dilation involves activation of the endothelium derived nitric oxide (EDNO) pathway. [14][15][16][17] However, it is unclear whether EDNO is involved in the human coronary responses to magnesium. Therefore, in the present study we investigated the coronary vascular responses to intracoronary infusion of magnesium.…”

mentioning

confidence: 99%

Magnesium causes nitric oxide independent coronary artery vasodilation in humans

Teragawa

Kato

Yamagata

et al. 2001

Heart

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OBJECTIVETo determine how magnesium affects human coronary arteries and whether endothelium derived nitric oxide (EDNO) is involved in the coronary arterial response to magnesium.DESIGNQuantitative coronary angiography and Doppler flow velocity measurements were used to determine the effects of the nitric oxide synthase inhibitorNG-monomethyl-L-arginine (L-NMMA) on magnesium induced dilation of the epicardial and resistance coronary arteries.SETTINGHiroshima University Hospital a tertiary cardiology centre.PATIENTS17 patients with angiographically normal coronary arteries.INTERVENTIONSMagnesium sulfate (MgSO4) (0.02 mmol/min and 0.2 mmol/min) was infused for two minutes into the left coronary ostium before and after intracoronary infusion of L-NMMA.MAIN OUTCOME MEASURESDiameter of the proximal and distal segments of the epicardial coronary arteries and coronary blood flow.RESULTSAt a dose of 0.02 mmol/min, MgSO4 did not affect the coronary arteries. At a dose of 0.2 mmol/min, MgSO4 caused coronary artery dilation (mean (SEM) proximal diameter 3.00 (0.09) to 3.11 (0.09) mm; distal 1.64 (0.06) to 1.77 (0.07) mm) and increased coronary blood flow (79.3 (7.5) to 101.4 (9.9) ml/min, p < 0.001 vbaseline for all). MgSO4 increased the changes in these parameters after the infusion of L-NMMA (p < 0.001v baseline).CONCLUSIONSMagnesium dilates both the epicardial and resistance coronary arteries in humans. Furthermore, the coronary arterial response to magnesium is dose dependent and independent of EDNO.

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Dietary magnesium improves endothelial dependent relaxation of balloon injured arteries in rats

Cited by 9 publications

References 17 publications

Magnesium in Man: Implications for Health and Disease

Magnesium in Man: Implications for Health and Disease

Ultrasound and magnetic resonance molecular imaging of atherosclerotic neovasculature with perfluorocarbon magnetic nanocapsules targeted against vascular endothelial growth factor receptor 2 in rats

Magnesium causes nitric oxide independent coronary artery vasodilation in humans

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