Dietary flaxseed oil supplementation ameliorates the effect of cisplatin on brush border membrane enzymes and antioxidant system in rat intestine

Naqshbandi, Ashreeb; Rizwan, Sana; Khan, M. Masroor A.; Khan, Farah

doi:10.1177/0960327112438929

Cited by 17 publications

(12 citation statements)

References 56 publications

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“…tGSH keeps sulfhydryl groups of molecules reduced, inhibiting their oxidation [29]. Our finding of decreased levels of sulfhydryl groups in the jejunal tissue of animals receiving cisplatin indicates that our results are compatible with the literature [30]. In the current study, a significant decrease was found in GPO activity in the jejunal tissue of animals that received cisplatin.…”

Section: Discussionsupporting

confidence: 92%

“…CAT is considered an enzymatic antioxidant; this substance inhibits the formation of toxic hydroxyl radicals by catalyzing the decomposition of hydrogen peroxide (H 2 O 2 ) to oxygen and water [32,33]. It has been reported that CAT activity is decreased in tissue damage induced by cisplatin [30]. Again, in our study, the levels of NO were significantly elevated in the jejunal tissue of cisplatin-administered rats.…”

Section: Discussionsupporting

confidence: 52%

“…As previously reported, decreased GPO activity causes a drop in reduced glutathione and a rise in oxidized glutathione (GSSG), leading to a decrease in the GSH-to-GSSG ratio [31]. Naqshbandi et al reported that cisplatin significantly reduced GPO activity in the jejunal tissue compared to healthy tissues [30]. In addition to these antioxidant parameters, cisplatin was also found to significantly suppress CAT activity.…”

Section: Discussionmentioning

confidence: 83%

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Effects of Hippophae rhamnoides extract on oxidative mucosal injury induced by cisplatin in rat jejunum

Arslan¹,

Özçıçek²,

Çimen³

et al. 2018

biomedicalresearch

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Objectives: We aimed to assess the effect of Hippophae rhamnoides extract (HR) on oxidative stress induced by cisplatin (Cis) in rat small intestine tissue by evaluating the biochemical and gene expression levels and histopathological changes. Materials and methods: The control group and Cis group received distilled water, while the HR25+cisplatin (HR25+Cis) group and the HR50+cisplatin (HR50+Cis) group were given HR 25 and 50 mg/kg, respectively, orally for seven days. HR25+Cis, HR50+Cis, Cis groups were injected with a single dose of intraperitoneal cisplatin on the first day. After sacrifice, the jejunum of each rat was removed for the assessment of oxidants and antioxidants. Analyses of the gene expression (for IL-1ß and TNF-α) and histopathological changes evaluated. Results: HR significantly inhibited the increase of oxidants and the decrease of antioxidants caused by cisplatin in the jejunal tissue. IL-1β and TNF-α gene expression levels were almost the same in both the HR50+Cis and the control groups. HR better prevented increase of the serum levels of IL-1β and TNF-α in animals at 50 mg/kg dose compared to 25 mg/kg dose. We confirmed that HR prevented the histopathological changes caused by cisplatin. Conclusions: It was concluded that oxidative stress caused by cisplatin may be preventable by coadministered HR.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 83%

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Effects of Hippophae rhamnoides extract on oxidative mucosal injury induced by cisplatin in rat jejunum

Arslan¹,

Özçıçek²,

Çimen³

et al. 2018

biomedicalresearch

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“…Similar results were obtained by Rossary et al (2007), who found that SOD activity did not change when fibroblasts were treated with DHA or EPA omega-3 polyunsaturated fatty acid. On the other hand, Naqshbandi et al (2012) showed an improvement in SOD activity in the intestine from rats treated with omega-3-enriched diet. The contradictory results of SOD activity may occur due to differences between experimental protocols, mainly related to the amount of omega 3 and/or the treatment duration.…”

Section: Discussionmentioning

confidence: 95%

Omega-3 fatty acids differentially modulate enzymatic anti-oxidant systems in skeletal muscle cells

Silva

Nachbar

Levada‐Pires

et al. 2016

Cell Stress and Chaperones

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During physical activity, increased reactive oxygen species production occurs, which can lead to cell damage and in a decline of individual's performance and health. The use of omega-3 polyunsaturated fatty acids as a supplement to protect the immune system has been increasing; however, their possible benefit to the anti-oxidant system is not well described. Thus, the aim of this study was to evaluate whether the omega-3 fatty acids (docosahexaenoic acid and eicosapentaenoic acid) can be beneficial to the anti-oxidant system in cultured skeletal muscle cells. C2C12 myocytes were differentiated and treated with either eicosapentaenoic acid or docosahexaenoic acid for 24 h. Superoxide content was quantified using the dihydroethidine oxidation method and superoxide dismutase, catalase, and glutathione peroxidase activity, and expression was quantified. We observed that the docosahexaenoic fatty acids caused an increase in superoxide production. Eicosapentaenoic acid induced catalase activity, while docosahexaenoic acid suppressed superoxide dismutase activity. In addition, we found an increased protein expression of the total manganese superoxide dismutase and catalase enzymes when cells were treated with eicosapentaenoic acid. Taken together, these data indicate that the use of eicosapentaenoic acid may present both acute and chronic benefits; however, the treatment with DHA may not be beneficial to muscle cells.

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“…Animal studies have shown that omega-3 fatty acid may possess potent antioxidant and anti-inflammatory properties and may prevent fibrosis of different organs [ 25 ]. It also empowers the antioxidant defense system by reducing oxidative stress and increasing the activities of the major antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase [ 26 ]. The American Heart Association recommends that all adults eat fish 2 to 3 times per week because of its content of ω -3FA.…”

Section: Introductionmentioning

confidence: 99%

Effect of Omega-3 Fatty Acid Alone and in Combination with Proprietary Chromium Complex on Endothelial Function in Subjects with Metabolic Syndrome: A Randomized, Double-Blind, Parallel-Group Clinical Study

Pingali

Nutalapati

Gundagani

2021

Evidence-Based Complementary and Alternative Medicine

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Metabolic syndrome (MetS) represents a cluster of metabolic abnormalities that include hypertension, central obesity, insulin resistance, and dyslipidemia and is strongly associated with an increased risk of diabetes, cardiovascular diseases (CVD), and all-cause mortality. Early diagnosis is important to employ lifestyle and risk factor modification. Existing therapies are limited. Studies report positive effect of omega-3 fatty acids (ω-3FA) on symptoms of metabolic syndrome. The present study was undertaken to evaluate the effect of ω-3FA alone and in combination with proprietary chromium complex (PCC) on endothelial function in subjects with metabolic syndrome. In this randomized, double-blind, parallel-group study, subjects were enrolled into the study after ethics committee (EC) approval and informed consent. Eligible subjects were randomized to receive ω-3FA concentrate 2000 mg (Group A-18 subjects), ω-3FA concentrate 2000 mg + PCC200 mcg (Group B-19 subjects), and ω-3FA concentrate 2000 mg + PCC400 mcg (Group C-21 subjects) daily for 12 weeks. Endothelial dysfunction as measured by reflection index (RI), biomarkers of oxidative stress (NO, MDA, and glutathione), and inflammation (hsCRP, endothelin-1, ICAM-1, and VCAM-1) were evaluated at baseline, 4, and 12 weeks. Lipid-profile and platelet-aggregation tests were performed at baseline and 12 weeks. Adverse drug reactions were recorded. Compliance was assessed by pill count method. GraphPad Prism8 was used for statistical analysis. Significant changes were seen from 4 weeks onwards in all the parameters evaluated. Significant improvement in RI% (mean ± SD = −2.56 ± 0.77 to −3.27 ± 0.67-group A, −2.33 ± 0.76 to 4.72 ± 0.79-group B; −2.39 ± 1.13 to 6.46 ± 1.00-group C) was seen at 12 weeks. Significant improvement in biomarkers of oxidative stress and inflammation was seen with all the treatment groups. Similarly, significant improvement in lipid profile was seen in group B and group C, while group A showed change in HDL, VLDL, and TG. Group C demonstrated the best response in the parameters evaluated. Three patients in group C reported gastrointestinal adverse events, which resolved spontaneously; none stopped the therapy. So, the addition of PCC to ω-3FA may prove to have beneficial effect in reducing cardiovascular morbidity in MetS patients.

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Dietary flaxseed oil supplementation ameliorates the effect of cisplatin on brush border membrane enzymes and antioxidant system in rat intestine

Cited by 17 publications

References 56 publications

Effects of Hippophae rhamnoides extract on oxidative mucosal injury induced by cisplatin in rat jejunum

Effects of Hippophae rhamnoides extract on oxidative mucosal injury induced by cisplatin in rat jejunum

Omega-3 fatty acids differentially modulate enzymatic anti-oxidant systems in skeletal muscle cells

Effect of Omega-3 Fatty Acid Alone and in Combination with Proprietary Chromium Complex on Endothelial Function in Subjects with Metabolic Syndrome: A Randomized, Double-Blind, Parallel-Group Clinical Study

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