Dietary Beta‐Hydroxy Beta‐Methyl Butyrate Supplementation Alleviates Liver Injury in Lipopolysaccharide‐Challenged Piglets

Duan, Yehui; Song, Bo; Zheng, Chunyan; Zhong, Yinzhao; Guo, Qiuping; Zheng, Jie; Yin, Yulong; Li, Jianjun; Li, Fengna

doi:10.1155/2021/5546843

Cited by 4 publications

(4 citation statements)

References 35 publications

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“…As an acute-phase protein, the serum levels of CRP can increase rapidly in response to tissue injury or infection ( 22 ). In this study, LPS injection induced elevated serum ALT and ALP concentrations in newborn piglets; the results agreed with many previous studies ( 23 – 25 ). The serum hs-CRP levels in the N-CON and N-LPS groups were lower than the C-LPS group.…”

Section: Discussionsupporting

confidence: 94%

Maternal nucleotide supplementation improves the intestinal morphology and immune function in lipopolysaccharide-challenged newborn piglets

Hung¹,

Bai²

2022

Front. Vet. Sci.

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This study aimed to evaluate the effects of maternal nucleotide (NT) supplementation on intestinal morphology and immune function in lipopolysaccharide-challenged newborn piglets. At 85 d gestation, 12 sows were selected and assigned to two groups: the CON group (basal diet, n = 6) and the NT group (basal diet with 1 g/kg NT mixture, n = 6). After parturition, newborn piglets were collected without suckling. Piglets from the CON group were intraperitoneally injected with sterile saline or lipopolysaccharide (LPS, 10 mg/kg body weight), and divided into the C-CON (n = 6) and C-LPS groups (n = 6). Piglets from the NT group received the same treatment and were divided into the N-CON (n = 6) and N-LPS groups (n = 6). The blood and small intestinal samples of piglets were collected 1 h after injection. The results showed that: (1) maternal NT supplementation increased the concentrations of serum complement C3 and C4 (P < 0.05), and suppressed the increase in serum hypersensitive C-reactive protein in LPS-challenged newborn piglets (P < 0.05); (2) maternal NT supplementation increased the villus height and the ratio of villus height to crypt depth in the duodenum of newborn piglets (P < 0.05) and inhibited the LPS-induced decrease in the villus height in the jejunum and ileum (P < 0.05). (3) The LPS-induced increased levels of interleukin-6 in the jejunum and tumor necrosis factor-α in the ileum of newborn piglets were suppressed by maternal NT supplementation (P < 0.05). (4) In the jejunum of newborn piglets, maternal NT supplementation inhibited the LPS-induced increase in toll-like receptor 4 (TLR4) mRNA and protein expression (P < 0.05) and the decrease of nuclear factor-κB inhibitor α (IκBα) protein expression (P < 0.05). In the ileum, piglets had a lower nuclear factor-κB (NFκB) mRNA expression in the NT groups than the CON groups (P < 0.05), and maternal NT supplementation suppressed the decrease of IκBα mRNA in LPS-treated piglets (P < 0.05). In conclusion, maternal NT supplementation could promote the intestinal development and immune function of newborn piglets, and may improve LPS-induced intestinal inflammatory responses via the TLR4/IκBα/NFκB pathway.

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Section: Discussionsupporting

confidence: 94%

Maternal nucleotide supplementation improves the intestinal morphology and immune function in lipopolysaccharide-challenged newborn piglets

Hung¹,

Bai²

2022

Front. Vet. Sci.

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“…LPS, as an immune stressor, can disrupt bowel function by affecting the expression of genes related to intestinal immune responses, growth, absorption, mucosal energy metabolism, and mucosal barrier function (23). The model of intestinal injury induced by LPS stimulation has been widely used to screen antibiotic substitutes (24)(25)(26)(27). To further evaluate whether CCT can be used as a potential antibiotic substitute in feed, colistin sulfate (CS), as an antibiotic growth promoter which has been extensively used as a feed additive to reduce pathogen infections and improve growth performance after weaning (28,29), was served as a positive control in this study.…”

Section: Introductionmentioning

confidence: 99%

Dietary supplementation with a complex of cinnamaldehyde, carvacrol, and thymol negatively affects the intestinal function in LPS-challenged piglets

Zhang

Wang

et al. 2023

Front. Vet. Sci.

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BackgroundThe effects of cinnamaldehyde, carvacrol and thymol complex (CCT) on the growth performance and intestinal function of piglets challenged with lipopolysaccharide (LPS) were determined. Colistin sulphate (CS) was as a positive control.MethodPiglets (n = 24, 32 days of age) were allocated to four treatments: Control group (fed basal diet), LPS group (fed basal diet), CS+LPS group (fed basal diet + 50 mg/kg CS), and CCT+LPS group (fed basal diet + 50 mg/kg CCT).ResultsResults showed that diarrhea rates of piglets were significantly reduced by CCT and CS supplementation respectively. Further research showed that CS supplementation tended to improve the intestinal absorption function in LPS-challenged piglets. Moreover, CS supplementation significantly reduced the contents of cortisol in blood and malondialdehyde in the duodenum and the activities of inducible nitric oxide synthase in the duodenum and ileum and total nitric oxide synthase in the ileum in LPS-challenged piglets. CS supplementation significantly increased the activities of sucrase in the ileum and myeloperoxidase in the jejunum in LPS-challenged piglets. CS supplementation significantly alleviated the reduced mRNA levels of immune-related genes (IL-4, IL-6, IL-8, IL-10) in mesenteric lymph nodes and jejunum and mucosal growth-related genes (IGF-1, mTOR, ALP) in LPS-challenged piglets. These results suggested that CS supplementation improved the intestinal function in LPS-challenged piglets by improving intestinal oxidative stress, immune stress, and absorption and repair function. However, although CCT supplementation improved oxidative stress by reducing (p < 0.05) the content of malondialdehyde and the activity of nitric oxide synthase in the duodenum, CCT supplementation tended to aggravate the intestinal absorption dysfunction in LPS-challenged piglets. Furthermore, compared with the control and LPS groups, CCT supplementation remarkably elevated the content of prostaglandin in plasma and the mRNA levels of pro-inflammatory factor IL-6 in mesenteric lymph nodes and jejunum, and reduced the activity of maltase in the ileum in LPS-challenged piglets. These results suggested that CCT supplementation had a negative effect on intestinal function by altering intestinal immune stress response and reducing disaccharidase activity in LPS-challenged piglets.ConclusionsCompared to CS, CCT supplementation exhibited a negative effect on intestinal function, suggesting whether CCT can be as an effective feed additive still needs further study.

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“…The challenge trial was conducted at 08.00 h on days 7, 11 and 14 of the experiment, piglets from CTRL þ LPS group and PQQ þ LPS group were received intraperitoneal injection of Escherichia coli Lipopolysaccharide (LPS, E. coli serotype 055: B5; Sigma Chemical) at a dose of 80 μg/kg body weight and others were offered equivalent amount vehicle (0•9 % NaCl). Three injections were chosen according to the previous study with minor modifications (17,18) . And the dose of LPS was set according to the previous research (19) .…”

Section: Methodsmentioning

confidence: 99%

Pyrroloquinoline quinone supplementation attenuates inflammatory liver injury by STAT3/TGF-β1 pathway in weaned piglets challenged with lipopolysaccharide

Huang,

Yu,

Shi

et al. 2023

Br J Nutr

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This study is aimed to evaluate the effect and underling mechanism of dietary supplementation with pyrroloquinoline quinone disodium (PQQ) on improving inflammatory liver injury in piglets challenged with lipopolysaccharide (LPS). A total of 72 crossbred barrows were allotted into 4 groups as following: the CTRL group (basal diet + saline injection); the PQQ group (3 mg/kg PQQ diet + saline injection); the CTRL + LPS group (basal diet + LPS injection); and the PQQ + LPS group (3 mg/kg PQQ diet + LPS injection). On d 7, 11 and 14, piglets were challenged with LPS or saline. Blood was sampled at 4 hours after the last LPS injection (d 14), then the piglets were slaughtered and liver tissue were harvested. The results showed that the hepatic morphology was improved in the PQQ + LPS group compared with the CTRL + LPS group. PQQ supplementation decreased level of serum inflammatory factors, AST, ALT, and increased HDL-C concentration in piglets challenged with LPS; piglets in PQQ + LPS group had lower liver mRNA level of inflammatory factors and protein level of α-SMA than in CTRL + LPS group; Besides, mRNA expression of STAT3/TGF-β1 pathway and protein level of p-STAT3(Tyr 705) were decreased, and mRNA level of PPARα and protein expression of p-AMPK in liver were increased in PQQ + LPS group compared to CTRL + LPS group (P < 0.05). In conclusion, dietary supplementation with PQQ alleviated inflammatory liver injury might partly via inhibition of the STAT3/TGF-β1 pathway in piglets challenged with LPS.

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Dietary Beta‐Hydroxy Beta‐Methyl Butyrate Supplementation Alleviates Liver Injury in Lipopolysaccharide‐Challenged Piglets

Abstract: The current study was performed to investigate whether dietary β-hydroxy-β-methylbutyrate (HMB) could regulate liver injury in a lipopolysaccharide- (LPS-) challenged piglet model and to determine the mechanisms involved. Thirty piglets ( 21 ± 2 days old , … Show more

Cited by 4 publications

References 35 publications

Maternal nucleotide supplementation improves the intestinal morphology and immune function in lipopolysaccharide-challenged newborn piglets

Maternal nucleotide supplementation improves the intestinal morphology and immune function in lipopolysaccharide-challenged newborn piglets

Dietary supplementation with a complex of cinnamaldehyde, carvacrol, and thymol negatively affects the intestinal function in LPS-challenged piglets

Pyrroloquinoline quinone supplementation attenuates inflammatory liver injury by STAT3/TGF-β1 pathway in weaned piglets challenged with lipopolysaccharide

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