Diel variation of bacterial productivity in seagrass (Zostera capricorni) beds measured by rate of thymidine incorporation into DNA

Moriarty, David; Pollard, Peter

doi:10.1007/bf00396917

Cited by 147 publications

(112 citation statements)

References 24 publications

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“…labeled vs. unlabeled) and cannot be used as rigorous proof that a particular subpopulation is nonresponsive to a specific substrate. In fact, similar microautoradiographic studies (Moriarty and Pollard 1982) showed that [3H]adenine is assimilated by diatoms and cyanobacteria even when added at nanomolar concentrations.…”

mentioning

confidence: 72%

Does adenine incorporation into nucleic acids measure total microbial production?: A response to comments by Fuhrman et al.1

Karl

Winn

1986

Limnology & Oceanography

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mentioning

confidence: 72%

Does adenine incorporation into nucleic acids measure total microbial production?: A response to comments by Fuhrman et al.1

Karl

Winn

1986

Limnology & Oceanography

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“…Hydrochloric acid (100 l of 25% v/v) was added for acidification of the extract, along with 100 l of a herring-sperm-DNA solution (5 mg L Ϫ1 ; Sigma-Aldrich) as coprecipitant for the labeled DNA Pollard 1981, 1982) and 65 l trichloracetic acid (TCA, 100%; Sigma-Aldrich) for precipitation of the macromolecules. Addition of a humic extract as coprecipitant for DNA and proteins Pollard 1981, 1982;Kaplan et al 1992) was not necessary because of the dark brown color of the sediment extract, indicating a high amount of humic substances. After cooling for at least 30 min on ice (visible formation of a precipitate), the samples were again centrifuged at 14,000 rpm for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Benthic bacterial secondary production measured via simultaneous 3H‐thymidine and ¹⁴C‐leucine incorporation, and its implication for the carbon cycle of a shallow macrophyte‐dominated backwater system

Kirschner

Velimirov

1999

Limnology & Oceanography

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The role of the benthic bacterial community in the carbon cycle of a temperate, macrophyte-dominated oxbow was investigated in a 2-yr study from 1994 to 1996. Simultaneous incorporation of 3 H-thymidine into DNA and 14 C-leucine into proteins was monitored as a tool for the measurement of bacterial secondary production in the aerobic zone of the sediment along with bacterial numbers, morphotype composition, cell volumes, biomass, frequency of dividing cells (FDC), and abiotic variables. Bacterial numbers ranged from 2.5 ϫ 10 9 to 8.5 ϫ 10 9 cells ml Ϫ1 , corresponding to bacterial biomass values of 64 and 288 g C ml Ϫ1, respectively. Both bacterial production methods yielded similar and reliable results over the whole investigation period, varying between 42 and 2,350 g C L Ϫ1 h Ϫ1 for the thymidine method and between 67 and 2,490 g C L Ϫ1 h Ϫ1 for the leucine method, and also corresponded well with the FDC values. Only during late spring and early summer was the thymidine uptake uncoupled from leucine incorporation. Temperature was found to be of significant importance for the variation of all bacterial parameters. In addition carbon inputs from the water column were apparently crucial stimulators of benthic bacterial growth. The spring phytoplankton bloom together with higher temperatures led to a strong positive response of the bacterial community in the sediment. However, the planktonic algal production was not sufficient to cover the benthic bacterial C-demand and, as derived from mass-balance calculations, decaying macrophytes of the previous season seemed to provide the main energy source until the middle of spring. From the middle of August to the beginning of October, the period of maximal biomass of submerged macrophytes, all bacterial parameters exhibited their annual maximum. The benthic bacterial community was dependent on leachates and decay products of the submerged vegetation during this time. Temperature limitation was most probably the reason for the low biomass and production values observed during autumn and winter.

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“…into the water column through leaching and exudation Water column parameters. Water temperature and and is subsequently utilized by heterotrophic bacteriodepth were recorded using a data logging system plankton (Moriarty & Pollard 1982, (YSI6000, YSI Inc.) secured at mid-depth (-0.5 m) for Chin-Leo & Benner 1991, Ziegler & Benner 1999). For the duration of each trip.…”

Section: Introductionmentioning

confidence: 99%

Nutrient cycling in the water column of a subtropical seagrass meadow

Ziegler¹,

Benner²

1999

Mar. Ecol. Prog. Ser.

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The* cycling of nutrients was studied over a 16 mo period to determine how processes occurring between the water column and benthos influenced nutrient dynamics in a Thalassia testudinum dominated seagrass meadow. Nutrient concentrations were low and ranged from below There was a significant correlation between the regeneration of NH4+ m the water column and the light-mediated release of dissolved organic matter (DOM) from the benthos, indicating that benthicderived DOM supported the regeneration of NH,' in the water column. Bacterioplankton growth effic~encies were significantly and positively correlated to the regeneration of NH4+ in the water column, possibly resulting from changes in the composition of DOM released from the benthos. The C:N ratios of the organic matter remineralized in the water column were variable and ranged from 14 to 81, with lowest values occurring in late summer and highest values in spring. The results of this study indicated that temporal variations in the source and composition of DOM significantly influenced the cycling of nutrients in the water column of this seagrass meadow.

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Diel variation of bacterial productivity in seagrass (Zostera capricorni) beds measured by rate of thymidine incorporation into DNA

Cited by 147 publications

References 24 publications

Does adenine incorporation into nucleic acids measure total microbial production?: A response to comments by Fuhrman et al.1

Does adenine incorporation into nucleic acids measure total microbial production?: A response to comments by Fuhrman et al.1

Benthic bacterial secondary production measured via simultaneous 3H‐thymidine and ¹⁴C‐leucine incorporation, and its implication for the carbon cycle of a shallow macrophyte‐dominated backwater system

Nutrient cycling in the water column of a subtropical seagrass meadow

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Diel variation of bacterial productivity in seagrass (Zostera capricorni) beds measured by rate of thymidine incorporation into DNA

Cited by 147 publications

References 24 publications

Does adenine incorporation into nucleic acids measure total microbial production?: A response to comments by Fuhrman et al.1

Does adenine incorporation into nucleic acids measure total microbial production?: A response to comments by Fuhrman et al.1

Benthic bacterial secondary production measured via simultaneous 3H‐thymidine and 14C‐leucine incorporation, and its implication for the carbon cycle of a shallow macrophyte‐dominated backwater system

Nutrient cycling in the water column of a subtropical seagrass meadow

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Benthic bacterial secondary production measured via simultaneous 3H‐thymidine and ¹⁴C‐leucine incorporation, and its implication for the carbon cycle of a shallow macrophyte‐dominated backwater system