Dichroa febrifuga Lour. inhibits the production of IL-1β and IL-6 through blocking NF-κB, MAPK and Akt activation in macrophages

Park, Sun Young; Park, Gayoung; Ko, Woo Shin; Kim, Young‐Hee

doi:10.1016/j.jep.2009.07.003

Cited by 23 publications

(15 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The data shown here were representative for three independent experiments with similar results LPS-stimulated mouse peritoneal macrophages. These effects are mediated by the inhibition of the activity of IKK/IjB/NF-jB signaling cascade [42]. Moreover, watersoluble extracts of Panellus serotinus (Mukitake) powder showed an inhibitory effect on IKKb, whose activation is required for NF-jB-mediated inflammatory response [40].…”

Section: Resultsmentioning

confidence: 99%

Anti-inflammatory, pro-apoptotic, and anti-proliferative effects of a methanolic neem (Azadirachta indica) leaf extract are mediated via modulation of the nuclear factor-κB pathway

et al. 2010

View full text Add to dashboard Cite

Azadirachta indica (neem tree) is used in traditional Indian medicine for its pharmacological properties including cancer prevention and treatment. Here, we studied a neem extract's anti-inflammatory potential via the nuclear factor-jB (NF-jB) signaling pathway, linked to cancer, inflammation, and apoptosis. Cultured human leukemia cells were treated with a methanolic neem leaf extract with or without tumor necrosis factor (TNF)-a stimulation. Inhibition of NF-jB activity was demonstrated by luciferase assay and electrophoretic mobility shift assay (EMSA). Inhibition of viability by neem extracts was assessed by luminescent assays. Western blot analysis allowed assessing the inhibitory effect of the neem extract on TNF-a-induced degradation of inhibitor of jB (IjB) and nuclear translocation of the NF-jB p50/p65 heterodimer. Inhibition of IjB kinase (IKK) activity was shown as well as the effect of neem extract on the induction of apoptotic cell death mechanisms by nuclear fragmentation analysis and flow cytometry analysis. In conclusion, our data provide evidence for a strong effect of the neem extract on proinflammatory cell signaling and apoptotic cell death mechanisms, contributing to a better understanding of the mechanisms triggered by Azadirachta indica.

show abstract

Section: Resultsmentioning

confidence: 99%

Anti-inflammatory, pro-apoptotic, and anti-proliferative effects of a methanolic neem (Azadirachta indica) leaf extract are mediated via modulation of the nuclear factor-κB pathway

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Based on recent evidence, however, attention has shifted to the role of Akt signaling in LPS-induced inflammatory responses [30] . It is noteworthy that anti-inflammatory herbal extracts from Eleutherococcus senticosus, Dichroa feb rifuga, and Cymbopogon citratus [31][32][33] and ethnopharmacological agents such as curcumin, resveratrol, and quercetin [34][35][36] may suppress the Akt pathway. The Akt inhibitors LY294002 and wortmannin displayed anti-inflammatory activity, reflected by the suppression of NO and PGE 2 ( Figure 6A).…”

Section: Resultsmentioning

confidence: 99%

8-(Tosylamino)quinoline inhibits macrophage-mediated inflammation by suppressing NF-κB signaling

et al. 2012

View full text Add to dashboard Cite

Aim:The macrophage-mediated inflammatory response may contribute to the development of cancer, diabetes, atherosclerosis and septic shock. This study was to characterize several new compounds to suppress macrophage-mediated inflammation. Methods: Peritoneal macrophages from C57BL/6 male mice and RAW264.7 cells were examined. Anti-inflammatory activity was evaluated in the cells exposed to lipopolysaccharide (LPS). The mechanisms of the anti-inflammatory activity were investigated via measuring transcription factor activation in response to specific signals and via assaying the activities of the target kinases. Results: Of 7 candidate compounds tested, 8-(tosylamino)quinoline (8-TQ, compound 7) exhibited the strongest activities in suppressing the production of NO, TNF-α, and PGE 2 in LPS-activated RAW264.7 cells and peritoneal macrophages (the IC 50 values=1-5 μmol/L). This compound (1.25-20 μmol/L) dose-dependently suppressed the expression of the pro-inflammatory genes for iNOS, COX-2, TNF-α, and the cytokines IL-1β and IL-6 at the level of transcription in LPS-activated RAW264.7 cells. 8-TQ (20 μmol/L) significantly suppressed the activation of NF-κB and its upstream signaling elements, including inhibitor of κB (IκBα), IκBα kinase (IKK) and Akt in LPSactivated RAW264.7 cells. In in vivo experiments, oral administration of 20 and 40 mg/kg 8-TQ for 3 d significantly alleviated the signs of LPS-induced hepatitis and HCl/EtOH-induced gastritis, respectively, in ICR mice. Conclusion: 8-TQ (compound 7) exerts significant anti-inflammatory activity through the inhibition of the Akt/NF-κB pathway, thus may be developed as a novel anti-inflammatory drug.

show abstract

“…All animal studies were conducted in accordance with the principles and procedures in the Pusan National University Institutional Animal Care and Use Committee guidelines. Thioglycollate (TG) broth (Brewer, DIFCO, Detroit, MI)-elicited macrophages were harvested 3 day after intraperitoneal injection of 2.5 ml TG into mice and isolated as reported previously (Park et al, 2009). Murine macrophage RAW 264.7 cells were maintained in Dulbecco's modified Eagle's medium supplemented with glutamine (1 mM) and 10% FBS at 37 1C in an atmosphere of 5% CO 2 .…”

Section: Cell Culturementioning

confidence: 99%

Achyranthes japonica exhibits anti-inflammatory effect via NF-κB suppression and HO-1 induction in macrophages

Bang

Kim

et al. 2012

Journal of Ethnopharmacology

Self Cite

View full text Add to dashboard Cite

Dichroa febrifuga Lour. inhibits the production of IL-1β and IL-6 through blocking NF-κB, MAPK and Akt activation in macrophages

Cited by 23 publications

References 37 publications

Anti-inflammatory, pro-apoptotic, and anti-proliferative effects of a methanolic neem (Azadirachta indica) leaf extract are mediated via modulation of the nuclear factor-κB pathway

Anti-inflammatory, pro-apoptotic, and anti-proliferative effects of a methanolic neem (Azadirachta indica) leaf extract are mediated via modulation of the nuclear factor-κB pathway

8-(Tosylamino)quinoline inhibits macrophage-mediated inflammation by suppressing NF-κB signaling

Achyranthes japonica exhibits anti-inflammatory effect via NF-κB suppression and HO-1 induction in macrophages

Contact Info

Product

Resources

About