Diazepam induced oxidative DNA damage in cultured human lymphocytes

Azab, Mohammad; Khabour, Omar F.; Alzoubi, Karem H.; Almomani, Duaa H.

doi:10.1016/j.jksus.2017.03.002

Cited by 7 publications

(4 citation statements)

References 32 publications

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“…33 Researchers have also revealed the involvement of free radical species as the other probable factors for initiating depression after diazepam treatment. 34,35 Indeed, the increased production of reactive oxygen species (ROS) triggers DNA damage and protein denaturation 36 as well as, it may also affect the activities of antioxidant enzymes including catalase, superoxide dismutase, and glutathione peroxidase. 37 These effects should be kept in mind for the possible beneficial impact of low dose gamma irradiation in depression treatment.…”

Section: Discussionmentioning

confidence: 99%

Low dose γ radiation enhances antidepressant effect of resveratrol: Behavioral and neurochemical studies

Karam

Radwan

2020

Environmental Toxicology

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The low dose of radiation (LDR) has received growing attention for its beneficial neuroprotective effect. This study was designed to investigate the enhancing effect of LDR on the antidepressant potential of resveratrol against diazepam‐induced depression in mice. Female mice divided into five groups; control, diazepam (2 mg/kg), LDR (0.5Gy) + diazepam, resveratrol (20 mg/kg) + diazepam, LDR + resveratrol+diazepam. Mice received diazepam showed depressive symptoms as evidenced by decreased locomotor activity in the open field and increased immobility time in the forced swimming and tail suspension tests integrated with a marked decline in biogenic amines (serotonin, norepinephrine, and dopamine) in brain tissues. These effects were ameliorated by LDR or resveratrol administration demonstrating an antidepressant activity. Interestingly, LDR triggered the antidepressant effect of resveratrol as it restored the changes in behavioral tests, neurotransmitters, and neuro‐histoarchitecture. In conclusion, these findings suggested that LDR could be considered as a novel adjuvant that augmented the resveratrol antidepressant effect and might serve as a potential therapeutic approach for depression.

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Section: Discussionmentioning

confidence: 99%

Low dose γ radiation enhances antidepressant effect of resveratrol: Behavioral and neurochemical studies

Karam

Radwan

2020

Environmental Toxicology

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“…24 It has been stated that long term Diazepam treatment is associated with oxidative tissue damage. 6 The mechanism for the increase in the number of astrocytes and the major decrease in GFAP-reactive astrocyte expression as observed in Plate 3 (group C) is not yet understood but it may be due to the neuroprotective effects of the phenolic compounds and flavonoids in the fruit extract which possesses strong radical scavenging properties. 11 Some studies have illustrated the high antioxidant potential of Averrhoa carambola fruit extract and its protection against free radical damage.…”

Section: Resultsmentioning

confidence: 98%

“…5 It is generally effective when used therapeutically, but when abused or engaged in long-term treatment could lead to oxidative stress which can be injurious to the brain. 6 Oxidative stress results from prooxidative/antioxidative imbalance in the body which could lead to tissue damage. 7 The central nervous system is extremely sensitive to free radical damage because of its relatively small antioxidant capacity.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Averrhoa carambola (Star Fruit) Aqueous Fruit Extract on the Hippocampal Astrocyte Expression Following Diazepam-Induced Neurotoxicity in Wistar Rats

2021

TJNPR

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“…28 Cells were then incubated in RPMI for additional 6 hr and treated with glyphosate during the second incubation period. Cultures were then processed as previously described 30 and levels of 8-OHdG assay were assessed using a specialized kit according to instruction supplied by the producer (Kit ID: ab201734, Abcam Inc, UK).…”

Section: -Ohdg Assaymentioning

confidence: 99%

Genotoxicity of Glyphosate on Cultured Human Lymphocytes

et al. 2022

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Glyphosate-based herbicides are the most used herbicides in the world. Despite being widely used, a dispute exists whether glyphosate-based herbicides have a negative effect on human health, particularly genotoxic effects. Therefore, the aim of this study was to investigate glyphosate genotoxicity on cultured human lymphocytes. Cultured human lymphocytes were treated with different concentrations of glyphosate (20, 40, and 200 µmol/L). Four toxicity measures were examined: frequency of chromosomal aberrations (CAs), frequency of sister-chromatid exchange (SCE), production of 8-OHdG, and cell kinetics analysis. The results show that glyphosate induced significant ( P < 0.05) increases in the levels of SCE at the highest used concentration (200 μmol/L). However, no significant elevation in SCE levels was observed at the lower examined concentrations (20 and 40 μmol/L). No significant changes in CA were detected at all examined concentrations ( P = 0.86). Also, glyphosate did not induce changes to the normal level of 8-OHdG at all examined concentrations ( P = 0.98). Last, no significant changes in either mitotic index or proliferative index were observed at any examined concentrations ( P > 0.05). The results collectively indicate a lack of genotoxicity and cytotoxicity of glyphosate in cultured human lymphocytes when dealing with environmentally relevant concentrations (20 and 40 μmol/L). However, being exposed to higher concentrations (200 μmol/L) led to slightly higher level of SCE. Therefore, we recommend cautionary measures when dealing with glyphosate-based herbicides for individuals, such as farmers, who may be extensively exposed to high concentrations of these herbicides.

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Diazepam induced oxidative DNA damage in cultured human lymphocytes

Cited by 7 publications

References 32 publications

Low dose γ radiation enhances antidepressant effect of resveratrol: Behavioral and neurochemical studies

Low dose γ radiation enhances antidepressant effect of resveratrol: Behavioral and neurochemical studies

The Effect of Averrhoa carambola (Star Fruit) Aqueous Fruit Extract on the Hippocampal Astrocyte Expression Following Diazepam-Induced Neurotoxicity in Wistar Rats

Genotoxicity of Glyphosate on Cultured Human Lymphocytes

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