Diaphragm muscle fiber function and structure in humans with hemidiaphragm paralysis

Welvaart, Willem N.; Paul, Marinus A.; Hees, Hieronymus W. H. van; Stienen, G. J. M.; Niessen, J. W. M.; Man, Frances S. de; Sieck, Gary C.; Vonk‐Noordegraaf, Anton; Ottenheijm, Coen A.C.

doi:10.1152/ajplung.00040.2011

Cited by 19 publications

(14 citation statements)

References 37 publications

(47 reference statements)

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“…There are a number of conditions that may induce unilateral hemidiaphragm paralysis including: pulmonary malignancy (Welvaart et al, 2011), postoperative thoracic surgery complications (Smith et al, 2013), indwelling central venous catheters (Takasaki and Arai, 2001), and high cervical spinal cord injury (Goshgarian, 2003; Gransee et al, 2013; Mantilla et al, 2013a; Mantilla et al, 2013b; Mantilla and Sieck, 2009; Miyata et al, 1995; Vinit et al, 2006; Zhan et al, 1997). In all these conditions, the substantial reserve capacity of the DIAm for generating force may be significantly compromised, even if ventilation appears unimpaired.…”

Section: Discussionmentioning

confidence: 99%

Impact of unilateral denervation on transdiaphragmatic pressure

Gill

Mantilla

Sieck

2015

Respiratory Physiology & Neurobiology

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Section: Discussionmentioning

confidence: 99%

Impact of unilateral denervation on transdiaphragmatic pressure

Gill

Mantilla

Sieck

2015

Respiratory Physiology & Neurobiology

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“…Diaphragm fiber cross-sectional area (CSA) was determined by means of immunohistochemical analyses with myosin heavy chain antibodies performed on cryosections of the biopsy specimens (5,6). Figure 1A demonstrates atrophy of slow-and fast-twitch diaphragm fibers in critically ill patients (CSA slow-twitch fibers: control patients, 3,284 6 793 mm 2 vs. critically ill patients, 2,328 6 763 mm 2 , P = 0.004; fast-twitch fibers: control patients, 2,766 6 606 mm 2 vs. critically ill patients, 1,819 6 527 mm 2 , P , 0.0001).…”

Section: Diaphragm Fiber Strength Is Reduced In Critically Ill Patienmentioning

confidence: 99%

Diaphragm Fiber Strength Is Reduced in Critically Ill Patients and Restored by a Troponin Activator

Hooijman

Beishuizen

Waard

et al. 2014

Am J Respir Crit Care Med

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“…Single fiber contractile measurements and experimental protocols were performed according to previously described methods (8,29,37,38) with minor modifications, as described below. While the cells were in relaxing solution, sarcomere length was set at 2.5 m using a fast Fourier transformation on a region of interest on the real-time camera image.…”

Section: Single Muscle Fiber Contractile Measurementsmentioning

confidence: 99%

Diaphragm weakness in pulmonary arterial hypertension: role of sarcomeric dysfunction

Manders¹,

Man²,

Handoko

et al. 2012

American Journal of Physiology-Lung Cellular and Molecular Phys

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Manders E, de Man FS, Handoko ML, Westerhof N, van Hees HW, Stienen GJ, Vonk-Noordegraaf A, Ottenheijm CA. Diaphragm weakness in pulmonary arterial hypertension: role of sarcomeric dysfunction. Am J Physiol Lung Cell Mol Physiol 303: L1070-L1078, 2012. First published September 7, 2012 doi:10.1152/ajplung.00135.2012.-We previously demonstrated that diaphragm muscle weakness is present in experimental pulmonary arterial hypertension (PH). However, the nature of this diaphragm weakness is still unknown. Therefore, the aim of this study was to investigate whether changes at the sarcomeric level contribute to diaphragm weakness in PH. For this purpose, in control rats and rats with monocrotaline-induced PH, contractile performance and myosin heavy chain content of demembranated single diaphragm fibers were determined. We observed a reduced maximal tension of 20% (P Ͻ 0.05), whereas tension cost was preserved in type 2X and 2B diaphragm fibers in PH compared with control. The reduced maximal tension was associated with a reduction of force generated per half-sarcomeric myosin heavy chain content. Additionally, reduced Ca 2ϩ sensitivity of force generation was found in type 2X fibers compared with control, which could exacerbate diaphragm muscle weakness at submaximal activation. No changes in maximal tension and Ca 2ϩ sensitivity of force generation were observed in fibers from the nonrespiratory extensor digitorum longus muscle. Together, these findings indicate that diaphragm weakness in PH is at least partly caused by sarcomeric dysfunction, which appears to be specific for the diaphragm. single fiber; cross bridge cycling kinetics; Ca 2ϩ sensitivity; myosin heavy chain PULMONARY ARTERIAL HYPERTENSION (PH) is characterized by a progressive increase in pulmonary vascular resistance, ultimately leading to right heart failure and death. Patients with PH hyperventilate at rest, during exercise, and sometimes even during sleep (19,27). As a consequence, inspiratory muscle activity increases substantially, which may ultimately lead to overloading of the inspiratory muscles and respiratory muscle weakness.Recent studies have found markedly lower maximal inspiratory pressures in patients with PH compared with control subjects (19,25). This indicates that the force-generating capacity of the inspiratory muscles is impaired. The presence of inspiratory muscle weakness in PH was further supported by recent work from our group on a rat model for PH, which revealed a significant reduction in twitch and maximal tetanic force generation of the diaphragm, the main muscle of inspiration (8). Data from De Man et al. (8) also suggests a significant reduction of maximal tension in diaphragm fibers of two patients with PH.Thus evidence is accumulating that the diaphragm is weakened in PH. However, the nature of this diaphragm weakness is unknown and might involve changes at the level of sarcoplasmic reticulum calcium cycling or content or at levels downstream in the excitation-contraction coupling process, i.e., at the sarcomeric le...

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Diaphragm muscle fiber function and structure in humans with hemidiaphragm paralysis

Abstract: Welvaart WN, Paul MA, van Hees HW, Stienen GJ, Niessen JW, de Man FS, Sieck GC, Vonk-Noordegraaf A, Ottenheijm CA. Diaphragm muscle fiber function and structure in humans with hemidiaphragm paralysis.

Cited by 19 publications

References 37 publications

Impact of unilateral denervation on transdiaphragmatic pressure

Impact of unilateral denervation on transdiaphragmatic pressure

Diaphragm Fiber Strength Is Reduced in Critically Ill Patients and Restored by a Troponin Activator

Diaphragm weakness in pulmonary arterial hypertension: role of sarcomeric dysfunction

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