Diagnostic ion‐oriented identification and simultaneous quantification of chemical components in Allium chinense G. Don

Abstract

Allium chinense G. Don, a popular edible condiment with reputation of Ganoderma lucidum in vegetables, exerts significant health effects for treating coronary disease but chemical compounds and corresponding contents still remain unclear. In this study, a total of 80 chemical compounds were detected in Allium chinense extracts based on a summarized fragmentation pattern, of which 32 were unambiguously identified with reference standards. Furthermore, a practical and feasible method was developed and validated for simultaneous quantification of 18 chemical compounds, of which 17 compounds were steroidal saponins, by ultra‐high performance liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry. In addition, it was known that the contents of quantitative compounds varied significantly among multiple Allium chinense samples. Moreover, chemometric analysis results suggested that chinenoside I, macrostemonoside B, and chinenoside II were the most important markers responsible for poor consistency. Taken altogether, this study would be helpful for the chemical authentication and quality control of Allium chinense samples.

Deciphering the benefits and intensity levels of primary metabolites from Allium macrostemon Bunge and Allium chinense G. Don

“…Actually, these secondary metabolites were biosynthesized by a series of enzymatic reactions of primary metabolites. The chemical profiles, visual spatial distribution, and absolute quantification of these secondary metabolites were presented in previous studies [ 6 , 12 , 13 , 17 , 20 , 29 ]. However, except amino acids, limited researches about their primary metabolites were reported.…”

“…were observed in AMB, while laxogenin in ACGD was the representative sapogenin [ 4 – 6 , 14 ]. Besides, there are significant differences about the content levels and anti-platelet aggregation activities of these saponins [ 4 – 6 , 13 , 17 ]. In the contrast, except some free amino acids [ 3 – 5 ], chemical profiles and content evaluation of other primary metabolites in AMB and ACGD still remain unknown.…”

Characterization of volatile organic compounds with anti-atherosclerosis effects in Allium macrostemon Bge. and Allium chinense G. Don by head space solid phase microextraction coupled with gas chromatography tandem mass spectrometry

“…Recently, network pharmacology analysis showed these components act on multiple signal pathways through synergistic effects (12). So far, the chemical similarities and differences of the dried bulbs of AMB and ACGD have been successfully deciphered including chemical profiles, content levels and metabolic pathways (1,10,(13)(14)(15). For the fresh bulbs of AMB and ACGD, sulfur-containing compounds are their main volatile organic compounds (VOCs), especially dimethyl trisulfide, methyl propyl disulfide, methyl allyl trisulfide, dimethyl disulfide (16)(17)(18).…”