Diagnostic and prognostic value of calcium oscillatory pattern analysis for patients with ICSI fertilization failure

Meerschaut, Frauke Vanden; Leybaert, Luc; Nikiforaki, Dimitra; Chang, Qian; Heindryckx, Björn; Sutter, Petra De

doi:10.1093/humrep/des368

Cited by 66 publications

(61 citation statements)

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“…Moreover, AOA strategies remarkably differ between IVF centers. As previously suggested by our group, the variability observed among protocols confounds the comparison of the efficiency of these methods (reviewed by Vanden Meerschaut et al 2013). Therefore, randomized comparative studies would be of benefit to select the most appropriate AOA strategy, contributing in turn to a robust standardization of the method.…”

Section: Aoa: Clinical Applicationsmentioning

confidence: 88%

Single Ca2+ transients vs oscillatory Ca2+ signaling for assisted oocyte activation: limitations and benefits

et al. 2018

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Section: Aoa: Clinical Applicationsmentioning

confidence: 88%

Single Ca2+ transients vs oscillatory Ca2+ signaling for assisted oocyte activation: limitations and benefits

et al. 2018

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“…Sperm cells were treated with Lysolecithin (0.02 %) before injection [19]. Intracytoplasmic calcium oscillations were read out via radiometric measurements applying FURA-2AM excitation ratio (lambda 340 and 380 nm).…”

Section: Mouse Oocyte Calcium Analysis (Moca)mentioning

confidence: 99%

“…Only intact oocytes were included for diagnosis. Outcome was analysed according to Vanden Meerschaut [19] and scoring of the following parameters was performed: total number of oocytes showing oscillations, number of calcium peaks per oocyte within 2 h (frequency), relative amplitude (change in the fluorescence ratio divided by the baseline ratio (ΔR/R)). Calcium patterns were classified as score 0 (no calcium spikes), score + (1-2 spikes), score ++ (3-10 spikes) and score +++ (>10 spikes) within 2 h immediately subsequent to ICSI.…”

Section: Mouse Oocyte Calcium Analysis (Moca)mentioning

confidence: 99%

“…There are seven cases reported of AOA after complete fertilization failure in normozoospermia [8][9][10][11][12]19]; in some the cause was not clearly identified [8,10], or was due to an oocyte factor [9]. Among those, six cases were treated using Ca 2+ ionophore [8,10,19]. In a case report of a normozoospermic patient [10], most sperm heads that appeared normal under light microscopy, exhibited abnormal nuclear vacuolization when analyzed by transmission electron microscopy (TEM).…”

Section: Icsi Failures In Normozoospermiamentioning

confidence: 99%

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PLCζ disruption with complete fertilization failure in normozoospermia

Durbán¹,

Barragán²,

Colodrón³

et al. 2015

J Assist Reprod Genet

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Purpose Intracytoplasmic sperm injection (ICSI) is widely used to achieve fertilization in the presence of severe male factor, resulting in high fertilization rates. Nevertheless, 1-3 % of couples experience complete fertilization failure after ICSI. When a male factor is identified, assisted oocyte activation (AOA) can help overcome fertilization failures. The objective of this study is to describe a case of repeated complete fertilization failures after ICSI with donor oocytes, and to investigate the molecular and functional aspects of phospholipase C zeta (PLCζ) protein in the patient semen. Methods The patient was a normozoospermic male who had previously fathered, through natural conception, four children by a different partner. Molecular and functional analysis of sperm-specific PLCζ in the patient and control samples by means of gene sequencing, immunocytochemistry, Western blot, mouse oocyte activation test (MOAT), and mouse oocyte calcium analysis (MOCA) were used.Results PLCζ expression levels and distribution were significantly disrupted, although MOAT and MOCA did not indicate a decrease in activation ability. Conclusions Normozoospermic males can have disrupted expression and distribution of PLCζ, and reduced activation ability after ICSI in human oocytes, despite their normal activation potential in functional testing using mouse oocytes. Discrepancy among molecular and functional data might exist, as mutations in the gene sequence may not be the only cause of alteration in PLCζ protein related to activation failures.

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“…Ce déficit peut être diagnostiqué cliniquement grâce à un test hétérospécifique « MOAT » (mouse oocyte activation test). Ce test consiste à injecter les spermatozoïdes des patients dans des ovocytes de souris et à mesurer les modifications de calcium intracellulaire dans les 3 heures qui suivent la fécondation hétérospécifique [22]. calciques (Ca 2+ ) dans les ovocytes, permettant la sortie de méiose de cet ovocyte et la fécondation, induisant l'activation de la transduction du signal et mimant l'action de la PLCζ sur celui-ci.…”

Section: Diagnostic éTiologiqueunclassified