Diagnosis of Theileria equi infections in horses in the Azores using cELISA and nested PCR

Baptista, Cláudio; Lopes, M. S.; Tavares, Ana Carolina Perota; Rojer, Horst; Kappmeyer, Lowell S.; Mendonça, Duarte; Machado, Artur da Câmara

doi:10.1016/j.ttbdis.2012.11.008

Cited by 25 publications

(14 citation statements)

References 12 publications

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“…Similarly, we have found T. equi in ticks of this species infesting horses. Previous studies conducted in Portugal report the detection of this pathogen in samples collected from horses (Baptista et al, 2013;Ribeiro et al, 2013) but, to date, no reports were found relating R. bursa with T. equi in our country. Equine piroplasmosis is endemic in the north of Portugal (Ribeiro et al, 2013) and it is estimated that 90% of the equine population worldwide lives in areas where equine piroplasmosis is present (Dewaal, 1992).…”

Section: Discussioncontrasting

confidence: 61%

Detection and phylogenetic characterization of Theileria spp. and Anaplasma marginale in Rhipicephalus bursa in Portugal

Ferrolho

Antunes

Santos

et al. 2016

Ticks and Tick-borne Diseases

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a b s t r a c tTicks are obligatory blood-sucking arthropod (Acari:Ixodida) ectoparasites of domestic and wild animals as well as humans. The incidence of tick-borne diseases is rising worldwide, challenging our approach toward diagnosis, treatment and control options. Rhipicephalus bursa Canestrini and Fanzago, 1877, a two-host tick widely distributed in the Palearctic Mediterranean region, is considered a multi-host tick that can be commonly found on sheep, goats and cattle, and occasionally on horses, dogs, deer and humans. R. bursa is a species involved in the transmission of several tick-borne pathogens with a known impact on animal health and production. The aim of this study was to estimate R. bursa prevalence in Portugal Mainland and circulating pathogens in order to contribute to a better knowledge of the impact of this tick species. Anaplasma marginale and Theileria spp. were detected and classified using phylogenetic analysis. This is the first report of Theileria annulata and Theileria equi detection in R. bursa ticks feeding on cattle and horses, respectively, in Portugal. This study contributes toward the identification of currently circulating pathogens in this tick species as a prerequisite for developing future effective anti-tick control measures.

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Section: Discussioncontrasting

confidence: 61%

Detection and phylogenetic characterization of Theileria spp. and Anaplasma marginale in Rhipicephalus bursa in Portugal

Ferrolho

Antunes

Santos

et al. 2016

Ticks and Tick-borne Diseases

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“…As 113 sero-positive animals did not show positive amplification in PCR for T. equi, this may be attributed to the presence of PCR inhibitors or parasite clearance from the circulating blood of the hosts (Allsopp et al 2007;Bhoora 2009;Bhoora et al 2010;Baptista et al 2013). ELISA is an antibody-based test, and it is a well known fact that antibodies persist for many weeks after active infection, but PCR targets the DNA of contemporary parasite.…”

Section: Discussionmentioning

confidence: 99%

“…ELISA is an antibody-based test, and it is a well known fact that antibodies persist for many weeks after active infection, but PCR targets the DNA of contemporary parasite. About four samples (2.22 %) of animals were in the early infection period wherein the antibody titres had not reached the detectable levels (Jaffer et al 2009;Bhoora et al 2010;Baptista et al 2013). Sampling time plays a critical role in the detection of circulating parasites, as the prepatent period for T. equi infections is 12-14 days (Mehlhorn and Schein 1998;Baptista et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Theileria equi and Babesia caballi infection of equids in Punjab, India: a serological and molecular survey

Sumbria

Singla

Sharma

2015

Trop Anim Health Prod

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A cross-sectional study was conducted in Submountain undulating, Undulating plain, Western and Western plain agro-climatic zones of Punjab province, India, to determine the prevalence, agreement between diagnostic tests and associated related risk factors of Theileria equi and Babesia caballi infection in equids (horses, donkey, mules). An overall prevalence of 14.14 and 0.0% of T. equi and B. caballi was recorded by multiplex polymerase chain reaction targeting 18S ribosomal RNA (rRNA) for both the parasites and 75 and 1.11% by competitive enzyme-linked immunosorbent assay in a representative sample of 180 animals. Only two animals with positive antibody titre from B. caballi and none with PCR indicated T. equi as the predominant haemoprotozoan responsible for equine piroplasmosis in the study area. Among the PCR-positive animals, presence of tick vectors in farm vicinity was the most influential associated with T. equi infection (P = 0.002; odds ratio (OR) 9.30; 95% confidence interval (CI) = 3.32-27.10). For animals with higher anti-T. equi antibody titres, strong association of sero-prevalence for T. equi was recorded with age, sex, usage, tick infestation and deworming/vaccination status of host animals and farm management strategies. The study has demonstrated the possible absence of B. caballi in both conducive and non-conducive areas of Punjab and demonstrated T. equi as the potential agent of equine piroplasmosis in Punjab.

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“…Surra is characterized by a fluctuating parasitaemia with periods of paroxysms and intermissions, anemia, edema of the legs, dehydration, fever, abortion, and incoordination, followed by paralysis of the hind limbs (Gill, 1991). Theileiriosis, caused by an apicomplexan parasite (Theileria equi), is clinically characterised by fever, haemolytic anemia, icterus, petechial haemorrhages of the mucous membranes etc., but these are variable and are non-specific (Baptista et al, 2013). The disease renders negative effects on the health of equids, decreasing their productivity and work efficacy.…”

Section: Introductionmentioning

confidence: 99%

Multiplex PCR for detection of Trypanosoma evansi and Theileria equi in equids of Punjab, India

Sumbria

Singla

Sharma

et al. 2015

Veterinary Parasitology

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Diagnosis of Theileria equi infections in horses in the Azores using cELISA and nested PCR

Cited by 25 publications

References 12 publications

Detection and phylogenetic characterization of Theileria spp. and Anaplasma marginale in Rhipicephalus bursa in Portugal

Detection and phylogenetic characterization of Theileria spp. and Anaplasma marginale in Rhipicephalus bursa in Portugal

Theileria equi and Babesia caballi infection of equids in Punjab, India: a serological and molecular survey

Multiplex PCR for detection of Trypanosoma evansi and Theileria equi in equids of Punjab, India

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