Diagnosis of Sarcina ventriculi-derived haemorrhagic abomasitis in lambs by histopathology and real-time PCR

Tuzçu, Mehmet; Tuzcu, Nevin; Akçakavak, Gökhan; ÇELİK, Zeynep

doi:10.2754/avb202291030227

Cited by 6 publications

(3 citation statements)

References 10 publications

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“…In these cases, methods based on amplification of the genomic DNA of the agent appear to be an important option in determining the appropriate treatment. Real-time PCR has become an important diagnostic method in the field of veterinary medicine in recent years due to its rapid results, ability to detect microorganisms such as Mycoplasma and Chlamydia that are difficult to detect with conventional methods, and its high sensitivity (Hyeran et al, 2006;Pantchev et al, 2010;Sibitz et al, 2011;Tuzcu et al, 2021;Tuzcu et al, 2022;Akçakavak et al, 2023;Tuzcu et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

A Molecular and Histopathological Study on Bronchopneumonia in Cats

Akçakavak,

Tuzcu,

Çelik

et al. 2024

MJAVL

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In this study, it was aimed to determine Bordetella bronchiseptica, Mycoplasma felis, Staphylococcus aureus and Chlamydia felis, which cause bronchopneumonia in cats, by Real-time PCR and to compare the pathological findings of the identified agents. The material of the study was constituted of paraffin blocks belonging to the lungs, of which 21 bronchopneumonia were detected in microscopic examination (with Hematoxylin Eosin (HE)) from a total of 78 cats samples brought to Selcuk University, Faculty of Veterinary Medicine, Department of Pathology for pathological diagnosis. Histopathologically, polymorphonuclear leukocytes (PMNL) and mononuclear cell infiltration (MCI) in the bronchi and bronchiolar lumens, desquamation in the bronchi and bronchiolar epithelium, PMNL infiltration with oedama in alveolar lumens and desquamated alveolar epithelium, PMNL infiltration in the interstitium, and peribronchi and peribronchiolar MCI, and pleuritis were detected. Real-time PCR analysis revealed Bordetella bronchiseptica in 3 (14.29%) cases, Mycoplasma felis in 3 (14.29%), Staphylococcus aureus in 5 (23.8%), and Chlamydia felis in 5 (23.8%). Morever, Mycoplasma felis and Staphylococcus aureus infection was detected in 1 case, and Staphylococcus aureus and Chlamydia felis mixed infection was observed in 1 case. Our results show that relevant agents can frequently be isolated in cases of feline bronchopneumonia.

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Section: Discussionmentioning

confidence: 99%

A Molecular and Histopathological Study on Bronchopneumonia in Cats

Akçakavak,

Tuzcu,

Çelik

et al. 2024

MJAVL

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“…Sarcina occurrences associated with the mammalian gastrointestinal microbiota were recorded in young ruminants [12,30,31], dogs [9], as well as cats [10], and primates [13,15,17,[32][33][34].…”

Section: Discussionmentioning

confidence: 99%

Species and Strain Variability among Sarcina Isolates from Diverse Mammalian Hosts

Makovska

Killer

Modrackova

et al. 2023

Animals

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Sarcina spp. has been isolated from the gastrointestinal tracts of diverse mammalian hosts. Their presence is often associated with host health complications, as is evident from many previously published medical case reports. However, only a handful of studies have made proper identification. Most other identifications were solely based on typical Sarcina-like morphology without genotyping. Therefore, the aim of this work was culture detection and the taxonomic classification of Sarcina isolates originating from different mammalian hosts. Sarcina-like colonies were isolated and collected during cultivation analyses of animal fecal samples (n = 197) from primates, dogs, calves of domestic cattle, elephants, and rhinoceroses. The study was carried out on apparently healthy animals kept in zoos or by breeders in the Czech Republic and Slovakia. Selected isolates were identified and compared using 16S rRNA gene sequencing and multi-locus sequence analysis (MLSA; Iles, pheT, pyrG, rplB, rplC, and rpsC). The results indicate the taxonomic variability of Sarcina isolates. S. ventriculi appears to be a common gut microorganism in various captive primates. In contrast, a random occurrence was also recorded in dogs. However, dog isolate N13/4e could represent the next potential novel Sarcina taxonomic unit. Also, a potentially novel Sarcina species was found in elephants, with occurrences in all tested hosts. S. maxima isolates were detected rarely, only in rhinoceroses. Although Sarcina bacteria are often linked to lethal diseases, our results indicate that Sarcina spp. appear to be a common member of the gut microbiota and seem to be an opportunistic pathogen. Further characterization and pathogenic analyses are required.

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“…Sections were stained with Hematoxylin and Eosin (H&E), Masson's Trichrome methods and examined under a light microscope. Immunohistochemistry staining was performed according to Tuzcu et al (2022). Paraffin extraction and rehydration processes were performed on the sections.…”

Section: Case Reportmentioning

confidence: 99%

A case of papillary and infiltrative urothelial carcinoma of the urinary bladder in a terrier dog

Akçakavak¹,

ÇELİK

Uzunlu

et al. 2023

Journal of Advances in VetBio Science and Techniques

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Urothelial carcinoma are malignant tumours originating from the epithelial layer of the urinary bladder. In this instance, a case of infiltrative type urothelial carcinoma in the urinary bladder of a 2-year-old terrier dog was defined histopathologically and immunohistochemically. The material of the presented case consisted of urinary bladder tissue samples that were surgically extirpated from a two-year-old terrier-breed female dog that applied to the Department of Surgery of the Faculty of Veterinary Medicine, Selcuk University with the complaint of hematuria. The tumour brought to the pathology laboratory was 11*10*12 cm in size and had finger-shaped extensions. Its outer surface was rough and hemorrhagic. Tissues were fixed in 10% buffered formalin and paraffin blocks were obtained by going through the necessary routine follow-up procedures. Afterwards, sections were taken and subjected to Hematoxylin-Eosin, Masson's Trichrome and immunohistochemical staining. As a result of the pathological and immunohistochemical examinations of the tumoral tissue samples taken from the urinary bladder of a two-year-old terrier dog, the diagnosis of infiltrative type urothelial carcinoma was reached, and the case was discussed with the information provided by the literature. In addition, immunohistochemically, intense PCNA and VEGF staining has been associated with malignancy.

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Diagnosis of Sarcina ventriculi-derived haemorrhagic abomasitis in lambs by histopathology and real-time PCR

Cited by 6 publications

References 10 publications

A Molecular and Histopathological Study on Bronchopneumonia in Cats

A Molecular and Histopathological Study on Bronchopneumonia in Cats

Species and Strain Variability among Sarcina Isolates from Diverse Mammalian Hosts

A case of papillary and infiltrative urothelial carcinoma of the urinary bladder in a terrier dog

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