Diagnosis of Leishmania (Leishmania) chagasi infection in dogs and the relationship with environmental and sanitary aspects in the municipality of Palmas, state of Tocantins, Brazil

Bigeli, Julio Gomes; Junior, Waldesse Piragé de Oliveira; Teles, Natália Melquie Monteiro

doi:10.1590/s0037-86822012000100005

Cited by 17 publications

(15 citation statements)

References 29 publications

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“…and 58% L. infantum) is almost similar to other studies also using peripheral blood, i.e. Bigeli et al (2012) blood. Leishmania parasites have a high number of kDNA minicircles copies (10,000 copies) per parasite, which increases the sensitivity of the assay (Quaresma et al 2009;Solc a et al 2012), as observed in this study with »10 0 parasite mL ¡1 .…”

Section: Discussionsupporting

confidence: 90%

Detection of Leishmania (L.) infantum in stray dogs by molecular techniques with sensitive species-specific primers

Silva

Richini-Pereira

Kikuti

et al. 2016

Veterinary Quarterly

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Background: Canine visceral leishmaniasis (CVL) is a worldwide parasitic zoonosis caused by Leishmania (Leishmania) infantum around the world. Canids are the definitive hosts and sand flies the intermediate hosts.Objective: To test the hypothesis that a new species-specific primers (Lch14:Lch15, targeting a multiple alignment for L. infantum kDNA minicircle) is an efficient diagnostic tool for L. infantum. Methods: The presence of L. infantum DNA was assessed in blood samples of 69 stray dogs using the conventional PCR (cPCR) and quantitative PCR (qPCR). Additional 50 lymph nodes and 50 bone marrow samples (positive and negative samples for parasitological tests) from dogs from endemic and nonendemic areas for CVL were also used. Results: L. infantum strains, and all positive lymph node and bone marrow samples for parasitological test gave positive results for cPCR and qPCR, presenting analytical sensitivity of »10 0 parasite mL ¡1 . For the blood samples, 40/69 (58%; CI 95%; 46%-69%) resulted positive for L. infantum in both tests. All positive samples were confirmed by sequencing. Conclusion: This study showed the importance of the specific detection of L. infantum based on species-specific primers by molecular techniques, highlighting the application as a confirmation method in epidemiological studies and to adopt the best control measures.

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Section: Discussionsupporting

confidence: 90%

Detection of Leishmania (L.) infantum in stray dogs by molecular techniques with sensitive species-specific primers

Silva

Richini-Pereira

Kikuti

et al. 2016

Veterinary Quarterly

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“…Worldwide, and in Brazil, the number of dogs infected with L. infantum varies from 20 to 90% (Coura-Vital et al, 2011;Bigeli et al, 2012); however, in the studied region, there was no record of this parasite affecting humans or animals. There is also no evidence of the presence of Lutzomyia longipalpis in the municipality of Ilhéus (Carvalho et al, 2010).…”

Section: Discussionmentioning

confidence: 81%

Leishmania (Viannia) braziliensis in dogs in Brazil: epidemiology, co-infection, and clinical aspects

et al. 2015

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ABSTRACT. Leishmaniasis is an endemic disease present in 98 countries. In Brazil, the northeast region accounts for approximately half of the cases in humans, and has experienced an increased number of positive cases in dogs. In this study, we investigated the epidemiology of canine leishmaniasis in the city of Ilhéus, Bahia, using serological and molecular techniques and evaluated the possible environmental risk factors and associated clinical signs. Blood samples were collected from 560 dogs in urban and peri-urban areas in Ilhéus, northeastern Brazil. Genomic DNA was extracted from the selected animals and 12063 Leishmania (Viannia) braziliensis in dogs ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 12062-12073 (2015) subjected to molecular analysis using Leishmania species-specific primers and diagnosis of Trypanosoma cruzi. A total of 54.72% of dogs were positive for Leishmania braziliensis, and animals positive for both Leishmania infantum and T. cruzi were not identified. Hematologic variables were not statistically associated with cases of L. braziliensis. However, the positive animal group showed lower red blood cell and platelet counts and higher levels of urea and serum creatinine. Few dogs presented clinical signs compatible with the presence of Leishmania. Age of more than 2 years and specific hair colors were associated with positive results for L. braziliensis. The geoclimatic characteristics of the region may improve parasite survival, reproduction, and vectors. This may explain the higher rate of dogs identified as positive in this study.

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“…The presence of dogs in intradomicile and not in peridomicile was observed during the period of capture. The presence of dogs inside the houses is also considered an important factor, as they can act as a food source for females, serving as potential reservoirs of the disease, according to studies of Bigeli; Oliveira-Júnior; Teles [25]. …”

Section: Discussionmentioning

confidence: 99%

Spatial distribution and environmental factors associated to phlebotomine fauna in a border area of transmission of visceral leishmaniasis in Mato Grosso do Sul, Brazil

Andrade

Silva

Cristaldo

et al. 2014

Parasites & Vectors

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BackgroundMato Grosso do Sul has been undergoing a process of urbanization which results in loss of native vegetation. This withdrawal makes vectors of man and domestic animals closer, causing changes in the epidemiology of diseases such as American Visceral Leishmaniasis. The aim of the study was to evaluate the phlebotomine fauna and environmental issues related to the transmission of AVL in Ponta Porã, Mato Grosso do Sul, between 2009 and 2010.MethodsVegetation of the urban area was evaluated by Normalized Difference Vegetation Index (NDVI), Normalized Difference Water Index (NDWI) and Soil Adjusted Vegetation Index (SAVI).ResultsThe results showed that the phlebotomine fauna of the city consists of five species, especially Lutzomyia longipalpis (Lutz and Neiva, 1912), the vector of Leishmania (Leishmania) infantum. Predominance of males was observed. The insects were captured in greater quantity in the intradomicile. Lu. longipalpis was the most frequent and abundant species, present throughout the year, with a peak population after the rainy season. Vectors can be found in high amounts in forest and disturbed environments.ConclusionsThe finding of Lu. longipalpis in regions with little vegetation and humidity suggests that the species is adapted to different sorts of environmental conditions, demonstrating its close association with man and the environment it inhabits. The tourist feature of Ponta Porã reinforces its epidemiological importance as a vulnerable city. The geographical location, bordering Paraguay through dry border, makes possible the existence of a corridor of vectors and infected dogs between the two countries.

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Diagnosis of Leishmania (Leishmania) chagasi infection in dogs and the relationship with environmental and sanitary aspects in the municipality of Palmas, state of Tocantins, Brazil

Cited by 17 publications

References 29 publications

Detection of Leishmania (L.) infantum in stray dogs by molecular techniques with sensitive species-specific primers

Detection of Leishmania (L.) infantum in stray dogs by molecular techniques with sensitive species-specific primers

Leishmania (Viannia) braziliensis in dogs in Brazil: epidemiology, co-infection, and clinical aspects

Spatial distribution and environmental factors associated to phlebotomine fauna in a border area of transmission of visceral leishmaniasis in Mato Grosso do Sul, Brazil

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