Diabetes and Advanced Glycoxidation End Products

Huebschmann, Amy G.; Regensteiner, Judith G.; Vlassara, Helen; Reusch, Jane E.B.

doi:10.2337/dc05-2096

Cited by 262 publications

(223 citation statements)

References 202 publications

(60 reference statements)

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“…Low plasma concentrations of albumin are known to have adverse effects, and high levels of glucose and free radicals may impair the biological properties of serum albumin through the formation of harmful adducts (14). The nonenzymatic covalent attachment of glucose molecules to protein and the subsequent free radical-mediated oxidation give rise to advanced glycoxidation end (AGE) products, of which methylglyoxal (MGO) is considered a key component through the Amadori rearrangement (15). Several reports indicate that AGE products are able at least in vitro to induce activation of various cells including platelets (16,17).…”

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confidence: 99%

Glycated Albumin With Loss of Fatty Acid Binding Capacity Contributes to Enhanced Arachidonate Oxygenation and Platelet Hyperactivity: Relevance in Patients With Type 2 Diabetes

et al. 2014

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High plasma concentrations of nonesterified fatty acids (NEFAs), transported bound to serum albumin, are associated with type 2 diabetes (T2D). The effects of albumin on platelet function were investigated in vitro. Modifications of albumin, such as those due to glycoxidation, were found in patients with T2D, and the consequences of these modifications on biological mechanisms related to NEFA handling were investigated. Mass spectrometry profiles of albumin from patients with T2D differed from those from healthy control subjects. Diabetic albumin showed impaired NEFA binding capacity, and both structural and functional alterations could be reproduced in vitro by incubating native albumin with glucose and methylglyoxal. Platelets incubated with albumin isolated from patients with T2D aggregated approximately twice as much as platelets incubated with albumin isolated from healthy control subjects. Accordingly, platelets incubated with modified albumin produced significantly higher amounts of arachidonate metabolites than did platelets incubated with control albumin. We concluded that higher amounts of free arachidonate are made available for the generation of active metabolites in platelets when the NEFA binding capacity of albumin is blunted by glycoxidation. This newly described mechanism, in addition to hypoalbuminemia, may contribute to platelet hyperactivity and increased thrombosis, known to occur in patients with T2D.

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Glycated Albumin With Loss of Fatty Acid Binding Capacity Contributes to Enhanced Arachidonate Oxygenation and Platelet Hyperactivity: Relevance in Patients With Type 2 Diabetes

et al. 2014

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“…Several studies have reported increases in HbA1c level to be directly proportional to the fasting serum glucose levels in T2DM subjects. [5][6][7][8][9][10] Lines of evidence have postulated an association between status of oxidative stress and T2DM. 11 We have observed a strong direct correlation between levels of MDA and RBS; MDA and HbA1c in T2DM subjects having poor glycemic control ("r" = 0.83; 0.78, Graphs 1 and 2).…”

Section: Discussionmentioning

confidence: 99%

Study of Oxidative Stress Status in Relation to Glycemic Index Fluctuations in Type II Diabetes Mellitus Patients

Arafeen¹,

Chatterjee²

2017

Indian Journal of Medical Biochemistry

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Background Oxidative stress is characterized by an increased generation of O2-derived molecules called reactive oxygen species that provoke critical, even irreversible, cell injury. Aim To evaluate oxidative stress status through measurement of malondialdehyde (MDA) and to analyze association of changes in MDA status with respect to fluctuations in glycemic control. Materials and methods A total of 112 subjects, both males and females, aged above 30 years were enrolled for this study, in which 81 had type II diabetes and 31 were without diabetes. Random blood sugar (RBS) was measured by glucose oxidase and peroxidase method. Serum MDA was measured by thiobarbituric acid reactive substances method. Glycated hemoglobin (HbA1c) was measured by ion exchange resin method. Results The MDA and HbA1c levels were increased in diabetics and were statistically significant. In all the studied groups, MDA was positively correlated with RBS and HbA1c. Conclusion and clinical significance The study suggests that MDA should be measured along with routine parameters of disease and the use of redox active antioxidants to tone down MDA levels may be evaluated to contribute in early and improvised clinical management of type II diabetes mellitus and also to delay the development of secondary complications of the disease. How to cite this article Arafeen S, Chatterjee M. Study of Oxidative Stress Status in Relation to Glycemic Index Fluctuations in Type II Diabetes Mellitus Patients. Indian J Med Biochem 2017;21(1):49-53.

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“…Glycoxidation corresponds to the radicalmediated oxidation reaction of both free and protein-bound sugars [13]. Amadori rearrangement of glycated protein leads to the formation of advanced glycoxidation (also termed advanced glycation) end products (AGEs) [14,15]. AGEs accumulate in the tissues of diabetic patients and contribute to the disease progression [3].…”

Section: Pre-laboratory Preparationmentioning

confidence: 99%

Oxidative damage in diabetics: Insights from a graduate study in La Reunion University

Girard¹,

Rondeau²,

Catan³

et al. 2014

Biochem Molecular Bio Educ

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Due to the growing incidence of diabetes in developed nations, there is a compelling case to be made for teaching graduate students more deeply about mechanisms underlying this disease. Diabetes is associated with enhanced oxidative stress and protein glycation via the covalent binding of glucose molecules. Albumin represents the major plasmatic protein and undergoes enhanced glycoxidative modifications in diabetic condition. La Reunion Island, a French department located in the Indian Ocean exhibit a growing incidence of diabetes. At the University of La Reunion, our research group named GEICO (Groupe d'Etude sur l'Inflammation Chronique et l'Obesite) participated to foster research and training in diabetes context and focuses on the impact of glycated albumin mediated oxidative stress on cell physiopathology. A laboratory course was designed by our group to introduce graduate students to cutting edge techniques in redox biology while providing insights into scientific processes and methods. This two weeks research laboratory training took place at CYROI, a local biotechnology center that provides advanced facilities for research, business, and education. Using histochemistry, molecular biology, biochemical techniques, student investigated oxidative damages in liver from leptin receptor deficient diabetic mice compared to control littermates. In addition, they used an in vitro model by assaying oxidative impact of glycated albumin on hepatoma carcinoma HepG2 cells. This article gives an overview of the organization and protocol used by the students during their two weeks training in the laboratory. Therefore, it may be helpful for teaching graduate students techniques used in research laboratory working on redox biology. V C 2014 by The International Union of Biochemistry and Molecular Biology, 42(5): 435-442, 2014.

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Diabetes and Advanced Glycoxidation End Products

Cited by 262 publications

References 202 publications

Glycated Albumin With Loss of Fatty Acid Binding Capacity Contributes to Enhanced Arachidonate Oxygenation and Platelet Hyperactivity: Relevance in Patients With Type 2 Diabetes

Glycated Albumin With Loss of Fatty Acid Binding Capacity Contributes to Enhanced Arachidonate Oxygenation and Platelet Hyperactivity: Relevance in Patients With Type 2 Diabetes

Study of Oxidative Stress Status in Relation to Glycemic Index Fluctuations in Type II Diabetes Mellitus Patients

Oxidative damage in diabetics: Insights from a graduate study in La Reunion University

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