Di- and tetracarboxylate derivatives of pyridines, bipyridines and terpyridines as luminogenic reagents for time-resolved fluorometric determination of terbium and dysprosium

Hemmilä, Ilkka; Mukkala, Veli-Matti; Latva, Martti; Kiilholma, Pentti

doi:10.1016/0165-022x(93)90029-n

Cited by 53 publications

(19 citation statements)

References 17 publications

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“…Anal. Calcd for CdC 15 (1) suitable for X-ray diffraction measurement were obtained from methanol and water solution at a ratio of 3 : 1 by slow evaporation in air at room temperature. .57-7.54 (dd, 4H).…”

Section: Syntheses and Characterizationsmentioning

confidence: 99%

“…The ability of tpy to chelate a wide range of metal ions has led to its incorporation in macrocyclic ligands [9][10][11][12]. Lanthanide complexes of tpy ligands with multiple pendant carboxylate groups are attracting interest as luminescent agents for protein labeling [13][14][15][16]. The design of multi-nucleating ligands incorporating two or more remote tpy metal-binding domains permit extension of this area of coordination chemistry from the molecular to the supramolecular level [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

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Studies on two cadmium(II) and two zinc(II) complexes of 4′-chloro-2,2′ : 6′,2″-terpyridine with 1 : 1 or 1 : 2 ratio of metal and ligand

You

Huang

Fan

et al. 2009

Journal of Coordination Chemistry

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Section: Syntheses and Characterizationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Studies on two cadmium(II) and two zinc(II) complexes of 4′-chloro-2,2′ : 6′,2″-terpyridine with 1 : 1 or 1 : 2 ratio of metal and ligand

You

Huang

Fan

et al. 2009

Journal of Coordination Chemistry

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“…This technique has been widely employed in immunoassays, nucleic acid hybridisation assays, receptor-ligand assays, enzyme activity measurements and in various other applications [1, 6–8]. The physicochemical properties of different enhancement solutions and chelates have been discussed in several papers [5, 9, 10–12]. …”

Section: Introductionmentioning

confidence: 99%

“…One-step assays with high concentrations of citrate, ethylenediaminetetra acetate (EDTA) or metal ions, require that the detector molecule is labelled with an extremely stable lanthanide chelate. Such assays are immunoassays based on citrate and EDTA plasma samples, enzyme activity assays relying on high concentrations of metal ions in order for the enzyme to be active, soil analysis with immunoassays, cytotoxicity assays and nucleic acid assays carried out partly at relatively high temperatures [7–10, 14]. …”

Section: Introductionmentioning

confidence: 99%

A dissociative fluorescence enhancement technique for one-step time-resolved immunoassays

et al. 2010

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The limitation of current dissociative fluorescence enhancement techniques is that the lanthanide chelate structures used as molecular probes are not stable enough in one-step assays with high concentrations of complexones or metal ions in the reaction mixture since these substances interfere with lanthanide chelate conjugated to the detector molecule. Lanthanide chelates of diethylenetriaminepentaacetic acid (DTPA) are extremely stable, and we used EuDTPA derivatives conjugated to antibodies as tracers in one-step immunoassays containing high concentrations of complexones or metal ions. Enhancement solutions based on different β-diketones were developed and tested for their fluorescence-enhancing capability in immunoassays with EuDTPA-labelled antibodies. Characteristics tested were fluorescence intensity, analytical sensitivity, kinetics of complex formation and signal stability. Formation of fluorescent complexes is fast (5 min) in the presented enhancement solution with EuDTPA probes withstanding strong complexones (ethylenediaminetetra acetate (EDTA) up to 100 mM) or metal ions (up to 200 μM) in the reaction mixture, the signal is intensive, stable for 4 h and the analytical sensitivity with Eu is 40 fmol/L, Tb 130 fmol/L, Sm 2.1 pmol/L and Dy 8.5 pmol/L. With the improved fluorescence enhancement technique, EDTA and citrate plasma samples as well as samples containing relatively high concentrations of metal ions can be analysed using a one-step immunoassay format also at elevated temperatures. It facilitates four-plexing, is based on one chelate structure for detector molecule labelling and is suitable for immunoassays due to the wide dynamic range and the analytical sensitivity.Figure

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“…Figure 1 illustrates both short-lived (nanoseconds) and long-lived fluorescence decay. Certain lanthanide metal chelates have exceptionally long fluorescence lifetimes (11)(12)(13), and the large difference in s enables effective suppression of background, greatly enhancing detection efficiency. Less than 1% of the microorganisms found in the environment respond to culture, and the detection of rare organisms in situ using conventional fluorescence techniques can be exceedingly difficult (14).…”

mentioning

confidence: 99%

High intensity solid‐state UV source for time‐gated luminescence microscopy

2006

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Background: The unique discriminative ability of immunofluorescent probes can be severely compromised when probe emission competes against naturally occurring, intrinsically fluorescent substances (autofluorophores). Luminescence microscopes that operate in the timedomain can selectively resolve probes with long fluorescence lifetimes (s > 100 ls) against short-lived fluorescence to deliver greatly improved signal-to-noise ratio (SNR). A novel time-gated luminescence microscope design is reported that employs an ultraviolet (UV) light emitting diode (LED) to excite fluorescence from a europium chelate immunoconjugate with a long fluorescence lifetime. Methods: A commercial Zeiss epifluorescence microscope was adapted for TGL operation by fitting with a time-gated image-intensified CCD camera and a highpower (100 mW) UV LED. Capture of the luminescence was delayed for a precise interval following excitation so that autofluorescence was suppressed. Giardia cysts were labeled in situ with antibody conjugated to a europium chelate (BHHST) with a fluorescence lifetime >500 ls. Results: BHHST-labeled Giardia cysts emit at 617 nm when excited in the UV and were difficult to locate within the matrix of fluorescent algae using conventional fluorescence microscopy, and the SNR of probe to autofluorescent background was 0.51:1. However in time-gated luminescence mode with a gate-delay of 5 ls, the SNR was improved to 12.8:1, a 25-fold improvement. Conclusion: In comparison to xenon flashlamps, UV LEDs are inexpensive, easily powered, and extinguish quickly. Furthermore, the spiked emission of the LED enabled removal of spectral filters from the microscope to significantly improve efficiency of fluorescence excitation and capture. q

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Di- and tetracarboxylate derivatives of pyridines, bipyridines and terpyridines as luminogenic reagents for time-resolved fluorometric determination of terbium and dysprosium

Cited by 53 publications

References 17 publications

Studies on two cadmium(II) and two zinc(II) complexes of 4′-chloro-2,2′ : 6′,2″-terpyridine with 1 : 1 or 1 : 2 ratio of metal and ligand

Studies on two cadmium(II) and two zinc(II) complexes of 4′-chloro-2,2′ : 6′,2″-terpyridine with 1 : 1 or 1 : 2 ratio of metal and ligand

A dissociative fluorescence enhancement technique for one-step time-resolved immunoassays

High intensity solid‐state UV source for time‐gated luminescence microscopy

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