Dexmedetomidine protects from post-myocardial ischaemia reperfusion lung damage in diabetic rats

Kip, Gülay; Çelik, Ali; Bilge, Mustafa; Alkan, Metin; Kiraz, Hasan Ali; Özer, Abdullah; Şıvgın, Volkan; Erdem, Özlem; Arslan, M; Kavutçu, Mustafa

doi:10.3402/ljm.v10.27828

Cited by 33 publications

(31 citation statements)

References 33 publications

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“…DEX had been shown to improve renal dysfunction and inflammation in renal ischemic injury 15 . Moreover, DEX attenuated myocardial I/R induced lung damage in STZinduced diabetic rats through the modulation of oxidative stress 20 . Previous study reported that DEX protected against brain and heart ischemia injury in diabetic rats by reducing oxidative stress and inflammation [16][17] .…”

Section: ■ Discussionmentioning

confidence: 93%

“…Previous researches demonstrated that may be related to oxidative stress 19 , inflammation activition 4 and apoptosis 5 . Effects of DEX with antioxidant and anti-inflammatory were shown in diabetic animals combined organs I/R [16][17]20 . In our present study, which in accordance with previous study, renal I/R in diabetic rats induced high levels of apoptosis and oxidative stress (indicated by MDA and SOD), enhanced release of IL-1β and TNF-α.…”

Section: ■ Discussionmentioning

confidence: 99%

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Dexmedetomidine protects against renal ischemia and reperfusion injury by inhibiting the P38-MAPK/TXNIP signaling activation in streptozotocin induced diabetic rats

et al. 2017

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Section: ■ Discussionmentioning

confidence: 93%

Section: ■ Discussionmentioning

confidence: 99%

Dexmedetomidine protects against renal ischemia and reperfusion injury by inhibiting the P38-MAPK/TXNIP signaling activation in streptozotocin induced diabetic rats

et al. 2017

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“…Clinical studies have demonstrated that DEX stabilizes perioperative stress-induced hyperglycemia and decreases IR in patients with diabetes; this may be related to a reduction in sympathetic activity, analgesia, and antiinflammatory response [13]. DEX exerted protective effects in lungs by reducing the levels of ERS in a model of ischemia-reperfusion injury [35], and was shown to be closely related to ERS caused by burns and glucose deprivation [12,14,16]. However, the effects of DEX on ERS in an IR model have not been previously reported.…”

Section: Discussionmentioning

confidence: 99%

Dexmedetomidine alleviates insulin resistance in hepatocytes by reducing endoplasmic reticulum stress

Liu

Zhu

et al. 2019

Endocrine

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Purpose Dexmedetomidine (DEX) stabilizes intraoperative blood glucose levels and reduces insulin resistance (IR), a common perioperative complication. However, the molecular mechanisms underlying these effects remain unclear. Since endoplasmic reticulum stress (ERS) is a mechanism of IR, this study sought to examine whether DEX can effectively alleviate IR by reducing ERS. Methods HepG2 and LO2 cells were treated with different concentrations of insulin. The glucose content assay and Cell Counting Kit-8 (CCK-8) were then employed to determine the optimal insulin concentration capable of inducing IR without affecting cell viability. Insulin-resistant hepatocytes were cultured with different concentrations of DEX for 24 h, and the glucose concentration in the supernatant was measured. ERS was assessed by qPCR and western blotting. The latter was also used to quantify the expression of phosphorylated protein kinase B (p-AKT), phosphoenolpyruvate carboxykinase (PEPCK), and glucose 6 phosphatase (G6Pase), which are key proteins involved in the action of insulin. Results After 48-h of culturing with 10 μg/mL insulin, glucose consumption in hepatocytes was found to be reduced. IR hepatocytes cultured with 10, 100, or 1000 ng/ml DEX for 24 h showed a concentration-dependent increase in glucose consumption. Elevated mRNA and protein levels of ERS markers binding immunoglobulin protein (BIP) and ER protein 29 (ERp29), were reversed by DEX treatment. Moreover, reduced p-AKT and increased PEPCK and G6Pase protein levels in IR hepatocytes were also restored following DEX treatment. Conclusion DEX may alleviate IR in hepatocytes by reducing ERS serving to restore insulin action via the IRS-1/PI3K/AKT pathway.

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“…They found that the lung injury was reduced in dexmedetomidine group. 21 Luo et al demonstrated that dexmedetomidine prevents apoptosis in rat I/R injury renal tubular structure located in decreased apoptosis of NRK-52E cells by affecting the function of gap junctions between cells. 22 Freeman et al have been showed dexmedetomidine significantly reduces apoptosis by preserving the structure of the neuronal structure in cell cultures compared to control group rats after aortic surgery and thus reduces the risk of paraplegia.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Dexmedetomidine on Ischemia-Reperfusion Injury in Rat Ovary

Kirazoğlu¹,

Uyanıkoğlu²,

Incebiyik³

2017

Turkiye Klinikleri J Lab Anim

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dnexal torsion is the fifth leading cause of emergency gynecological surgery in spite of being seen rare. It is often seen in women with reproductive age, although it can be seen in prepubertal and postmenopausal period.1 Early diagnosis and treatment plays an important role in maintain the fertilization. Laparoscopy is a precise diagnosis and treatTurkiye Klinikleri J Lab Anim 2017;1(2):71-7 71Protective Effects of Dexmedetomidine on Ischemia-Reperfusion Injury in Rat Ovary A AB BS ST TR RA AC CT T O Ob bj je ec ct ti iv ve e: : Aim of this study is to evaluate the protective effect of dexmedetomidine on the histopathology and biochemical parameters in reducing damage in ischemia/reperfusion injury in rats ovary and to compare the effect of two different doses of dexmedetomidine. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Forty-two adult female Wistar Albino rats were used. Rats were separated into six groups: Sham, Torsion, Torsion+Detorsion, Torsion+Detorsion+Salin, Torsion+Detorsion+ Dexmedetomidine 10 µg/kg, and Torsion+Detorsion+Dexmedetomidine 20 µg/kg. Exception of the Sham group, ovarian torsion procedure was performed to all groups for 2 hours. Detorsion procedure was implemented to all groups for 2 hours, exception of Torsion group. Medications were given intraperitoneally half an hour before the detorsion procedure in Salin, Dexmedetomidine 10 µg and 20 µg groups. Then 2 mL of blood samples were drawn for markers of oxidative stress. The right ovaries were separated from all rats. R Re es su ul lt ts s: : The oxidative stress levels and histologic scores values of ovarian tissues were higher in Torsion+Detorsion group than in Sham group (p<0.001). By the uses of dexmedetomidine 10 and 20 µg, a significant decrease was established in the mean levels of oxidant markers and histopathologic scores. C Co on nc cl lu us si io on n: : Administration of dexmedetomidine 10 µg and 20 µg are effective in preventing tissue damage by ischemia\reperfusion injury in ovarian torsion. However, there was no difference between the dexmedetomidine 10 µg and 20 µg treatments with regards to protective activity. K Ke ey yw wo or rd ds s: : Ovary; dexmedetomidine; reperfusion injury Ö ÖZ ZE ET T A Am ma aç ç: : Bu çalışmanın amacı rat overlerinde deneysel olarak oluşturulan iskemi\reperfüzyon hasarına karşı deksmedetomidin tedavisinin koruyucu etkinliğini değerlendirmek ve iki farklı deksmedetomidin dozlarını karşılaştırmaktır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Bu çalışmada 42 tane dişi Wistar Albino rat kullanıldı. Ratlar her biri yedişer rat olmak üzere 6 gruba ayrıldı: Sham opere, Torsiyon, Torsiyon-Detorsiyon, Torsiyon+Detorsiyon+ İzotonik sodyum klorür, Torsiyon-Detorsiyon+ Deksmedetomidin 10 µg ve Torsiyon-Detorsiyon+ Deksmedetomidin 20 µg. Sham opere grubu hariç diğer tüm gruplara iki saat boyunca ovaryan torsiyon işlemi uygulandı. Torsiyon grubu dışındaki diğer tüm gruplara 2 saat detorsiyon prosedürü uygulandı. Salin, Deksmedetomidin 10 µg ve Deksmedetomidin 20 µg gruplarında y...

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Dexmedetomidine protects from post-myocardial ischaemia reperfusion lung damage in diabetic rats

Cited by 33 publications

References 33 publications

Dexmedetomidine protects against renal ischemia and reperfusion injury by inhibiting the P38-MAPK/TXNIP signaling activation in streptozotocin induced diabetic rats

Dexmedetomidine protects against renal ischemia and reperfusion injury by inhibiting the P38-MAPK/TXNIP signaling activation in streptozotocin induced diabetic rats

Dexmedetomidine alleviates insulin resistance in hepatocytes by reducing endoplasmic reticulum stress

Protective Effects of Dexmedetomidine on Ischemia-Reperfusion Injury in Rat Ovary

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