Développement et validation d’une méthode quantitative de dosage par Chromatographie Liquide à Haute Performance Ultra-violet (CLHP-UV) de l’artémisinine dans &lt;i&gt;Artemisia annua&lt;/i&gt; cultivé au Bénin

Ganse, Hippolyte; Gbaguidi, Fernand; Aminou, Taofiki; Zime, Hermine; Moudachirou, Mansourou; Quetin-Leclercq, Joëlle

doi:10.4314/ijbcs.v5i1.68092

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…The recovery rate found in this study was 98% (with a correlation coefficient of R = 0.99). This result corroborates a study [24] that separated amygdalin within 15 mn in almond seeds and food products in the UK. The result is similar to that of [25], who worked on artemisinin grown in Benin.…”

Section: Discussionsupporting

confidence: 92%

“…While, among cassava derivatives, the Agbeli derivative was more concentrated in amygdalin with a range of 401.56 µg/10 g. These contents are lower than the 141.000 µg 10 g −1 and 155.000 µg 10 g −1 obtained, respectively, with almond seeds and Mongolian almonds reported in the studies of [26] in China. High levels were also received with green plum, apricot, black plum, peach, red cherry, and black cherry, as reported by [24] (17.5 mg g −1 , 14.4 mg g −1 , 10 mg g −1 , 6.8 mg g −1 , 3.9 mg g −1 and 2.7 mg g −1 , respectively). The differences observed with these results can be explained by variations in the plant species studied, climatic and environmental factors, and the soil types exploited to grow these plants.…”

Section: Discussionmentioning

confidence: 59%

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Antioxidant, Anti-Inflammatory, and Anti-Cancer Properties of Amygdalin Extracted from Three Cassava Varieties Cultivated in Benin

Lehmane,

Kohonou,

Tchogou

et al. 2023

Molecules

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Given that cancer is a disease that is rampant in the world and especially in Africa, where the population has enormous difficulty treating it, plants are a safer and less expensive alternative. Cassava is a plant species valued in Benin because of its numerous medicinal and nutritional virtues. This study evaluated the biological activities of amygdalin from the organs of three cassava varieties most commonly produced in Benin (BEN, RB, and MJ). HPLC analysis was used to quantify amygdalin in cassava organs and derivatives. Phytochemical screening was performed to determine secondary metabolite groups. DPPH and FRAP methods were used to assess antioxidant activity. Cytotoxicity of the extracts was tested on Artemia salina larvae. The anti-inflammatory activity was evaluated in vivo in an albino mouse paw edema model induced by 5% formalin. The anticancer activity was evaluated in vivo on Wistar rats rendered cancerous by 1,2-dimethylhydrazine (DMH) using 5-fluorouracil as a reference molecule. The results showed that the organs of all three-cassava varieties contained glycosides, flavonoids, saponosides, steroids, tannins, coumarins, and cyanogenic derivatives. Young stems and fresh cassava leaves had the highest amygdalin concentrations, with 11,142.99 µg 10 g−1 and 9251.14 µg 10 g−1, respectively. The Agbeli derivative was more concentrated in amygdalin, with a content of 401.56 µg 10 g−1 than the other derivatives. The antioxidant activity results showed that the amygdalin extracts were DPPH radical scavengers with IC50 values ranging from 0.18 mg mL−1 to 2.35 mg mL−1. The cytotoxicity test showed no toxicity of the extracts toward shrimp larvae. Administration of amygdalin extracts from the leaves of BEN and MJ varieties prevents inflammatory edema. The percentages of edema inhibition varied between 21.77% and 27.89%. These values are similar (p > 0.05) to those of acetylsalicylic acid (25.20%). Amygdalin extract of the BEN variety significantly (p < 0.0001) reduces edema. Both BEN extracts inhibited cancer induction with DMH. In preventive and curative treatments, rats fed with amygdalin extracts showed low anti-cancer activity under the effect of DMH and a significant difference in biochemical results. Thus, the organs of all three cassava varieties studied have secondary metabolites and good antioxidant activity. The leaves contain high levels of amygdalin and can be used as anti-inflammatory and anticancer agents.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 59%

Antioxidant, Anti-Inflammatory, and Anti-Cancer Properties of Amygdalin Extracted from Three Cassava Varieties Cultivated in Benin

Lehmane,

Kohonou,

Tchogou

et al. 2023

Molecules

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“…Quant à la limite de détection (LD), elle correspond à la plus petite quantité de la substance considérée qui peut être détectée dans un échantillon, sans forcément pouvoir être quantifiée de façon exacte (ICH, 2005). Dans notre étude, la LQ et la LD ont été déterminées à travers les formules suivantes : LQ = 10 s/a et LD = 3 s/a avec s étant l'écarttype de la droite d'étalonnage et a étant la pente de cette droite (Ganse et al, 2011).…”

Section: Limite De Quantification Et Limite De Détectionunclassified

Développement et validation d’une méthode simple et rapide pour le dosage plasmatique de l’éfavirenz par chromatographie liquide haute performance

Thiombiano,

Yabre,

Kam

et al. 2023

Int. J. Bio. Chem. Sci

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L’éfavirenz est un inhibiteur sélectif non nucléosidique de la transcriptase inverse du virus de l’immunodéficience humaine de type 1 (VIH-1) se caractérisant par un index thérapeutique étroit et une forte variabilité inter-individuelle dans ses paramètres pharmacocinétiques. L’objectif de ce travail était de développer et valider une méthode rapide et simple de dosage plasmatique de l’éfavirenz par chromatographie liquide haute performance. Le développement a été effectué en utilisant une colonne C8 (100 x 4,6 mm, 5 μm) et une phase mobile constituée d’un mélange acétonitrile-eau, (55-45 v/v). Le débit et la longueur d’onde de détection étaient respectivement de 1 mL/min et 246 nm. Le temps de rétention de l’éfavirenz était de 6,2 minutes. Aucun étalon interne n’a été utilisé dans cette méthode. L’étude de validation a montré que la méthode mise au point était spécifique, linéaire, exacte et précise dans l’intervalle de concentration de 0,5 à 10 mg/L. La méthode ainsi développée et validée pourrait être utilisée pour déterminer la concentration plasmatique de l’éfavirenz dans le cadre d’un suivi thérapeutique pharmacologique des patients sous antirétroviraux. Efavirenz is an anti-viral agent non-nucleoside reverse transcriptase inhibitor category used for the treatment of infections of human immune deficiency virus type-1 (HIV-1). It is characterized by a narrow therapeutic window and a high variability in its pharmacokinetic parameters. The objective of this work consisted of developing and validating a rapid and simple method for determination of efavirenz in human plasma using high performance liquid chromatography. The method was developed using a C8 column (100 x 4.6 mm, 5 μm) and a mobile phase consisting of acetonitrile and water 55/45, v/v. A flow rate of 1 mL/min and a wavelength of 246 nm was used. The retention time of efavirenz was 6.2 min. No internal standard was employed. The validation study showed that the developed method was selective, linear, accurate et precise over the concentration range of 0.5-10 mg/L. The developed and validated method could be used for the therapeutic and pharmacokinetic monitoring of efavirenz.

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Antioxidant, Anti-Inflammatory and Anti-Cancer Properties of Amygdalin Extracted from Three Cassava Varieties Cultivated in Benin

Lehmane¹,

Kohonou²,

Tchogou³

et al. 2023

Preprint

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: Given that cancer is a disease that is rampant in the world and especially in Africa where the population has enormous difficulty in treating it, plants are a safer and less expensive alterna-tive. Cassava is one of the plant species valued in Benin because of its numerous medicinal and nutritional virtues. This study evaluated the biological activities of amygdalin from the organs of three cassava varieties most produced in Benin (BEN, RB, and MJ). HPLC analysis was used to quantify amygdalin in cassava organs and derivatives. Phytochemical screening was performed to determine secondary metabolite groups. DPPH and FRAP methods were used to assess anti-oxidant activity. Cytotoxicity of the extracts was tested on Artemia salina larvae. The an-ti-inflammatory activity was evaluated in vivo on albino mouse paw edema model induced by 5% formalin. The anticancer activity was evaluated in vivo on Wistar rats rendered cancerous by 1,2-dimethylhydrazine (DMH) using 5-fluorouracil as reference molecule. The results showed that the organs of all three-cassava varieties contained glycosides, flavonoids, saponosides, ster-oids, tannins, coumarins, and cyanogenic derivatives. Young stems and fresh leaves of cassava had the highest amygdalin concentration with 11142.99 µg 10 g-1 and 9251.14 µg 10 g-1 respec-tively. The Agbeli derivative was more concentrated in amygdalin with a content of 401.56 µg 10 g-1 than the others derivatives. The antioxidant activity results showed that the amygdalin ex-tracts were found to be DPPH radical scavengers with IC50 values ranging from 0.18 mg mL-1 to 2.35 mg mL-1. The cytotoxicity test showed no toxicity of the extracts toward shrimp larvae. Ad-ministration of amygdalin extracts from the leaves of BEN and MJ varieties prevents inflamma-tory edema. The percentages of edema inhibition varied between 21.77% and 27.89%. These val-ues are similar (p> 0.05) to that of acetylsalicylic acid (25.20%). Amygdalin extract of BEN variety significantly (p<0.0001) reduces edema. Cancer induction with DMH was inhibited by both BEN extract. In both preventive and curative treatments, rats fed with amygdalin extracts showed low anti-cancer activity under the effect of DMH and the significant difference in biochemical results. Thus, the organs of all three cassava varieties studied have secondary metabolites and good an-tioxydant activity. The leaves contain high levels of amygdalin and can be used as an-ti-inflammatory and anticancer agents.

show abstract

Développement et validation d’une méthode quantitative de dosage par Chromatographie Liquide à Haute Performance Ultra-violet (CLHP-UV) de l’artémisinine dans <i>Artemisia annua</i> cultivé au Bénin

Cited by 3 publications

References 5 publications

Antioxidant, Anti-Inflammatory, and Anti-Cancer Properties of Amygdalin Extracted from Three Cassava Varieties Cultivated in Benin

Antioxidant, Anti-Inflammatory, and Anti-Cancer Properties of Amygdalin Extracted from Three Cassava Varieties Cultivated in Benin

Développement et validation d’une méthode simple et rapide pour le dosage plasmatique de l’éfavirenz par chromatographie liquide haute performance

Antioxidant, Anti-Inflammatory and Anti-Cancer Properties of Amygdalin Extracted from Three Cassava Varieties Cultivated in Benin

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