Developmental pattern of expression of the mouse α1(XI) collagen gene (<i>Col11a1</i>)

Yoshioka, Hidekatsu; Iyama, Ken‐ichi; Inoguchi, Kazuhito; Khaleduzzaman, Mohammed; Ninomiya, Yoshifumi; Ramirez, Francesco

doi:10.1002/aja.1002040106

Cited by 71 publications

(47 citation statements)

References 35 publications

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“…We demonstrated that CBF/NF-Y bound to the Ϫ147 to Ϫ121 region but could not find any protein-DNA complex in the Ϫ199 to ϩ271 region. These data are not consistent with the previous experiments using DNA footprinting techniques, in which three areas, FP1 (Ϫ63 to Ϫ51), FP2 (Ϫ111 to Ϫ95), and FP3 (Ϫ171 to Ϫ159), were protected using the nuclear extracts from a primary line of smooth muscle cells, called 1120 (14). We do not know the reason for this discrepancy so far.…”

Section: Figcontrasting

confidence: 92%

The Transcription Factor CCAAT-binding Factor CBF/NF-Y Regulates the Proximal Promoter Activity in the Human α1(XI) Collagen Gene (COL11A1)

Matsuo¹,

Wang²,

Sumiyoshi³

et al. 2003

Journal of Biological Chemistry

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We have characterized the proximal promoter region of the human COL11A1 gene. Transient transfection assays indicate that the segment from ؊199 to ؉1 is necessary for the activation of basal transcription. Electrophoretic mobility shift assays (EMSAs) demonstrated that the ATTGG sequence, within the ؊147 to ؊121 fragment, is critical to bind nuclear proteins in the proximal COL11A1 promoter. We demonstrated that the CCAAT binding factor (CBF/NF-Y) bound to this region using an interference assay with consensus oligonucleotides and a supershift assay with specific antibodies in an EMSA. In a chromatin immunoprecipitation assay and EMSA using DNA-affinity-purified proteins, CBF/NF-Y proteins directly bound this region in vitro and in vivo. We also showed that four tandem copies of the CBF/NF-Ybinding fragment produced higher transcriptional activity than one or two copies, whereas the absence of a CBF/NF-Y-binding fragment suppressed the COL11A1 promoter activity. Furthermore, overexpression of a dominant-negative CBF-B/NF-YA subunit significantly inhibited promoter activity in both transient and stable cells. These results indicate that the CBF/NF-Y proteins regulate the transcription of COL11A1 by directly binding to the ATTGG sequence in the proximal promoter region.The collagen superfamily, one of the extracellular matrix proteins, plays an important role, not only in stabilizing the tissues as structural components but also in regulating a variety of biological functions, such as development, differentiation, proliferation, and morphogenesis (1-3). Among them, types I, II, III, V, and XI collagens are included in the group of fibril-forming collagens, based on their structural and functional features, and are divided into two subgroups, major (I, II and III) and minor (V and XI) fibrillar collagen (4) on the basis of their contents in tissues.Type XI collagen is a component of the collagen fibrillar network found in cartilage (5), and consists of three genetically distinct polypeptide chains: ␣1(XI), ␣2(XI), and ␣3(XI); the last of these is thought to be overglycosylated ␣1(II) chains (6).Although type XI collagen is a relatively minor collagen and is buried within the major collagen fibrils, it is important for the regulation of fibril diameter (7). Chondrodysplasia mice (cho), which do not synthesize ␣1(XI) chains, show irregular collagen fibrils in their cartilage (8). Contrary to the previous findings, the ␣1(XI) chain is not restricted to cartilage (9 -14), and it was demonstrated that the ␣1(XI) chain could form a heterotrimer with the ␣2(V) chain in a 2:1 ratio in non-cartilaginous cells and tissues (15,16). Although the precise function of this crosstype trimer remains unclear, the ␣1(XI) collagen gene is more broadly expressed than other collagen genes, showing that the ␣1(XI) collagen gene is the sole collagen gene to be expressed in both cartilaginous and non-cartilaginous tissues. This implies that the regulation of the ␣1(XI) collagen gene might be more complex than expected.We have previously re...

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Section: Figcontrasting

confidence: 92%

The Transcription Factor CCAAT-binding Factor CBF/NF-Y Regulates the Proximal Promoter Activity in the Human α1(XI) Collagen Gene (COL11A1)

Matsuo¹,

Wang²,

Sumiyoshi³

et al. 2003

Journal of Biological Chemistry

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“…Sheet-embedded cells also expressed genes encoding for components of the embryonic cardiac extracellular matrix (laminin, collagens I, XI, and XVIII) [29][30][31]. These findings indicate that, in some respect, a three-dimensional engineered tissue could mimic the natural environment allowing progenitor cells embedded in a proper extracellular matrix to stably display a very lowrate division and apoptosis [23].…”

Section: Discussionmentioning

confidence: 85%

Human Cardiac Progenitor Cell Grafts as Unrestricted Source of Supernumerary Cardiac Cells in Healthy Murine Hearts

et al. 2011

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Human heart harbors a population of resident progenitor cells that can be isolated by stem cell antigen-1 antibody and expanded in culture. These cells can differentiate into cardiomyocytes in vitro and contribute to cardiac regeneration in vivo. However, when directly injected as single cell suspension, less than 1%-5% survive and differentiate. Among the major causes of this failure are the distressing protocols used to culture in vitro and implant progenitor cells into damaged hearts. Human cardiac progenitors obtained from the auricles of patients were cultured as scaffoldless engineered tissues fabricated using temperature-responsive surfaces. In the engineered tissue, progenitor cells established proper three-dimensional intercellular relationships and were embedded in self-produced extracellular matrix preserving their phenotype and multipotency in the absence of significant apoptosis. After engineered tissues were leant on visceral pericardium, a number of cells migrated into the murine myocardium and in the vascular walls, where they integrated in the respective textures. The study demonstrates the suitability of such an approach to deliver stem cells to the myocardium. Interestingly, the successful delivery of cells in murine healthy hearts suggests that myocardium displays a continued cell cupidity that is strictly regulated by the limited release of progenitor cells by the adopted source. When an unregulated cell source is added to the system, cells are delivered to the myocardium. The exploitation of this novel concept may pave the way to the setup of new protocols in cardiac cell therapy.

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“…In the developing mouse, the gene was expressed in the vertebrae, in the osteoblasts of the trabecular bone and in the chondroid tissue of the intervertebral disc (Yoshioka et al, 1995). In salmon it was found in the osteoblast of the trabecular and compact bone, even though expression was much higher in notochordal chordoblasts (Fig.…”

Section: A Wargelius and Othersmentioning

confidence: 99%

Collagen type XI α1 may be involved in the structural plasticity of the vertebral column in Atlantic salmon (Salmo salar L.)

Wargelius

Fjelldal

Nordgarden

et al. 2010

Journal of Experimental Biology

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SUMMARY Atlantic salmon (Salmo salar L.) vertebral bone displays plasticity in structure, osteoid secretion and mineralization in response to photoperiod. Other properties of the vertebral bone, such as mineral content and mechanical strength, are also associated with common malformations in farmed Atlantic salmon. The biological mechanisms that underlie these changes in bone physiology are unknown, and in order to elucidate which factors might be involved in this process, microarray assays were performed on vertebral bone of Atlantic salmon reared under natural or continuous light. Eight genes were upregulated in response to continuous light treatment, whereas only one of them was upregulated in a duplicate experiment. The transcriptionally regulated gene was predicted to code for collagen type XI α1, a protein known to be involved in controlling the diameter of fibrillar collagens in mammals. Furthermore, the gene was highly expressed in the vertebrae, where spatial expression was found in trabecular and compact bone osteoblasts and in the chordoblasts of the notochordal sheath. When we measured the expression level of the gene in the tissue compartments of the vertebrae, the collagen turned out to be 150 and 25 times more highly expressed in the notochord and compact bone respectively, relative to the expression in the trabecular bone. Gene expression was induced in response to continuous light, and reduced in compressed vertebrae. The downregulation in compressed vertebrae was due to reduced expression in the compact bone, while expression in the trabecular bone and the notochord was unaffected. These data support the hypothesis that this gene codes for a presumptive collagen type XI α1, which may be involved in the regulatory pathway leading to structural adaptation of the vertebral architecture.

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Developmental pattern of expression of the mouse α1(XI) collagen gene (Col11a1)

Cited by 71 publications

References 35 publications

The Transcription Factor CCAAT-binding Factor CBF/NF-Y Regulates the Proximal Promoter Activity in the Human α1(XI) Collagen Gene (COL11A1)

The Transcription Factor CCAAT-binding Factor CBF/NF-Y Regulates the Proximal Promoter Activity in the Human α1(XI) Collagen Gene (COL11A1)

Human Cardiac Progenitor Cell Grafts as Unrestricted Source of Supernumerary Cardiac Cells in Healthy Murine Hearts

Collagen type XI α1 may be involved in the structural plasticity of the vertebral column in Atlantic salmon (Salmo salar L.)

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