Developmental effects on ureide levels are mediated by tissue-specific regulation of allantoinase in Phaseolus vulgaris L.

Díaz‐Leal, Juan Luis; Gálvez‐Valdivieso, Gregorio; Fernández, Javier; Pineda, Manuel; Alamillo, Josefa M.

doi:10.1093/jxb/ers090

Cited by 42 publications

(37 citation statements)

References 45 publications

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“…The relative independence of ureide-degrading activities and transcript amounts for ALN and AAH from environmental conditions, in particular the status of nitrogen fixation, had been noted earlier (Charlson et al, 2009;Alamillo et al, 2010;Díaz-Leal et al, 2012). ALN activity was independent of tissue ureide influx and ureide content, while AAH activity was only about 50% lower in soybean plants not relying on fixed nitrogen (Winkler et al, 1988;Rice et al, 1990).…”

Section: Discussionmentioning

confidence: 64%

“…However, there are two ALN genes in the genome of common bean, PvALN1 and PvALN2. PvALN2 is most similar to ALN3 and ALN4 from soybean, and as with these, its transcript is almost exclusively present in the nodules (Díaz-Leal et al, 2012). Interestingly, the transcript of PvALN2 contains one out-of-frame and two in-frame AUG codons upstream of the predicted start codon in the 59 leader sequence (according to data from Phytozome V8 [www.phytozome.…”

Section: Discussionmentioning

confidence: 94%

“…Under nonfixing conditions, significantly less ureides are found in the xylem (McClure and Israel, 1979;do Amarante et al, 2006). During germination, ureides possibly serve to export nitrogen from the cotyledons to the shoot axis (Quiles et al, 2009;Duran and Todd, 2012), and during senescence, they remobilize nitrogen from the vegetative tissue to the seeds (Thomas et al, 1980;Aveline et al, 1995;Díaz-Leal et al, 2012). When ureides are utilized as transport compounds, they must be taken up and hydrolyzed in the target tissue to gain access to the nitrogen.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The Ureide-Degrading Reactions of Purine Ring Catabolism Employ Three Amidohydrolases and One Aminohydrolase in Arabidopsis, Soybean, and Rice

et al. 2013

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

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The Ureide-Degrading Reactions of Purine Ring Catabolism Employ Three Amidohydrolases and One Aminohydrolase in Arabidopsis, Soybean, and Rice

et al. 2013

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“…Thus, ureide accumulation may be a more widespread response to water deficit not necessarily related to the regulation of NF (Alamillo et al ., 2010). In this regard, the level of ureides has been recently shown to depend on the plant developmental stage rather than on the growth conditions (nitrogen-fixing versus nitrate-fed) (Diaz-Leal et al ., 2012). …”

Section: Discussionmentioning

confidence: 99%

Local inhibition of nitrogen fixation and nodule metabolism in drought-stressed soybean

Gil‐Quintana

Larrainzar

Seminario

et al. 2013

Journal of Experimental Botany

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105

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Drought stress is a major factor limiting symbiotic nitrogen fixation (NF) in soybean crop production. However, the regulatory mechanisms involved in this inhibition are still controversial. Soybean plants were symbiotically grown in a split-root system (SRS), which allowed for half of the root system to be irrigated at field capacity while the other half remained water deprived. NF declined in the water-deprived root system while nitrogenase activity was maintained at control values in the well-watered half. Concomitantly, amino acids and ureides accumulated in the water-deprived belowground organs regardless of transpiration rates. Ureide accumulation was found to be related to the decline in their degradation activities rather than increased biosynthesis. Finally, proteomic analysis suggests that plant carbon metabolism, protein synthesis, amino acid metabolism, and cell growth are among the processes most altered in soybean nodules under drought stress. Results presented here support the hypothesis of a local regulation of NF taking place in soybean and downplay the role of ureides in the inhibition of NF.

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“…In nitrogen fixing conditions, French bean depends on ureide supply to obtain all the nitrogen required in developing tissues. French bean shows maximum nitrogen fixation levels at 28 days after sowing, just before the onset of flowering (Diaz-Leal et al, 2012). At that point, nucleotidase activity and PvNTD1 gene expression were very low in all tissues except in developing leaves.…”

Section: (Dxdx[t/v][l/v/i]) M(ii) ( § § § [T/s] Being § Hidrophobicmentioning

confidence: 95%

Identification and characterization of a gene encoding for a nucleotidase from Phaseolus vulgaris

Cabello-Díaz

Gálvez‐Valdivieso

Caballo

et al. 2015

Journal of Plant Physiology

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Nucleotidases are phosphatases that catalyse the removal of phosphate from nucleotides, compounds with an important role in plant metabolism. A phosphatase enzyme, with high affinity for nucleotides monophosphate previously identified and purified in embryonic axes from French bean, has been analysed by MALDI TOF/TOF and two internal peptides have been obtained. The information of these peptide sequences has been used to search in the genome database and only a candidate gene that encodes for the phosphatase was identified (PvNTD1). The putative protein contains the conserved domains (motif I to IV) for haloacid dehalogenase-like hydrolases superfamily. The residues involved in the catalytic activity are also conserved. A recombinant protein overexpressed in Escherichia coli has shown molybdate resistant phosphatase activity with nucleosides monophosphate as substrate, confirming that the identified gene encodes for the phosphatase with high affinity for nucleotides purified in French bean embryonic axes. The activity of the purified protein was inhibited by adenosine. The expression of PvNTD1 gene was induced at the specific moment of radicle protrusion in embryonic axes. The gene was also highly expressed in young leaves whereas the level of expression in mature tissues was minimal.

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Developmental effects on ureide levels are mediated by tissue-specific regulation of allantoinase in Phaseolus vulgaris L.

Cited by 42 publications

References 45 publications

The Ureide-Degrading Reactions of Purine Ring Catabolism Employ Three Amidohydrolases and One Aminohydrolase in Arabidopsis, Soybean, and Rice

The Ureide-Degrading Reactions of Purine Ring Catabolism Employ Three Amidohydrolases and One Aminohydrolase in Arabidopsis, Soybean, and Rice

Local inhibition of nitrogen fixation and nodule metabolism in drought-stressed soybean

Identification and characterization of a gene encoding for a nucleotidase from Phaseolus vulgaris

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