Developmental Control of Nuclear Size and Shape by kugelkern and kurzkern

Brandt, Annely; Papagiannouli, Fani; Wagner, Nicole; Wilsch‐Bräuninger, Michaela; Braun, Martina; Furlong, Eileen E. M.; Loserth, Silke; Wenzl, Christian; Pilot, Fanny; Vogt, Nina; Lecuit, Thomas; Krohne, Georg; Großhans, Jörg

doi:10.1016/j.cub.2006.01.051

Cited by 115 publications

(152 citation statements)

References 29 publications

Supporting

Mentioning

144

Contrasting

Order By: Relevance

“…Notably, all the nuclei are spherical at interphase in the precellular blastoderm. Asymmetric nuclear shapes emerge upon cellularization, and some of the molecules in nuclear morphogenesis have been identified (Brandt et al 2006;Pilot et al 2006). The evolution of the signatures of nuclear prestress was monitored in a transgenic fly constitutively expressing histone H2B tagged to EGFP (Bhattacharya et al 2006(Bhattacharya et al , 2009.…”

Section: Emergence Of Nuclear Prestress In the Context Of Developmentmentioning

confidence: 99%

Emergence of a prestressed eukaryotic nucleus during cellular differentiation and development

Mazumder

Shivashankar

2010

J. R. Soc. Interface.

View full text Add to dashboard Cite

Nuclear shape and size are emerging as mechanistic regulators of genome function. Yet, the coupling between chromatin assembly and various nuclear and cytoplasmic scaffolds is poorly understood. The present work explores the structural organization of a prestressed nucleus in a variety of cellular systems ranging from cells in culture to those in an organism. A combination of laser ablation and cellular perturbations was used to decipher the dynamic nature of the nucleo-cytoplasmic contacts. In primary mouse embryonic fibroblasts, ablation of heterochromatin nodes caused an anisotropic shrinkage of the nucleus. Depolymerization of actin and microtubules, and inhibition of myosin motors, resulted in the differential stresses that these cytoplasmic systems exert on the nucleus. The onset of nuclear prestress was then mapped in two contexts-first, in the differentiation of embryonic stem cells, where signatures of prestress appeared with differentiation; second, at an organism level, where nuclear or cytoplasmic laser ablations of cells in the early Drosophila embryo induced a collapse of the nucleus only after cellularization. We thus show that the interplay of physical connections bridging the nucleus with the cytoplasm governs the size and shape of a prestressed eukaryotic nucleus.

show abstract

Section: Emergence Of Nuclear Prestress In the Context Of Developmentmentioning

confidence: 99%

Emergence of a prestressed eukaryotic nucleus during cellular differentiation and development

Mazumder

Shivashankar

2010

J. R. Soc. Interface.

View full text Add to dashboard Cite

show abstract

“…2 Through a series of subsequent protein-protein interaction events with components of the NPCs, NLS-containing proteins translocate to the nuclear compartment. 1,2 The continuous discovery of novel NLSs 3,4 in recent years indicates the existence of yet-to-be identified NLSs in the Drosophila proteome. A sensitive nuclear import reporter would therefore be beneficial for further identification and characterization of NLSs.…”

Section: Introductionmentioning

confidence: 99%

A Green Fluorescent Protein-Based Reporter for Protein Nuclear Import Studies in Drosophila Cells

Chan

Shaw²,

Chan³

2007

Fly

View full text Add to dashboard Cite

“…32 Apart from lamins, the only farnesylated NM protein described so far is the Drosophila INM protein kugelkern (Kuk). 33 The polypeptide structure of Kuk is somewhat similar to the one of lamins, containing a putative N-terminal coiledcoil motif and a C-terminal CaaX motif. Apart from the structural similarities, Kuk shares functional similarities with lamins, 12 Other examples of dramatically altered nuclear morphology in differentiating cells are the nuclei of spermatocytes 13 and of myocytes.…”

Section: Farnesylated Nuclear Membrane Proteins Affect Nuclear Shape mentioning

confidence: 96%

“…1A-C). 18,33,34 Furthermore, overexpression of Kuk in the adult fly results in shorter lifespan and aging related phenotypes similarly as when LaΔ50/ progerin is expressed in mice or humans. 18 Reduced Kuk levels also affect nuclear morphology.…”

Section: Determining Nuclear Shapementioning

confidence: 99%

“…In kuk deficient cellularizing Drosophila embryos, the nuclei fail to elongate and do not show apical NM ruffling. 33 Furthermore, the nuclei seem to be detached from the centrosomes 35 which might indicate that Kuk mediates the connection of NM to cytoskeletal elements. The activity of Kuk on nuclear elongation seems to be specific, since the kuk mutant phenotype cannot be rescued by overexpression of lamin Dm0.…”

Section: Determining Nuclear Shapementioning

confidence: 99%

See 1 more Smart Citation

Determining nuclear shape: The role of farnesylated nuclear membrane proteins

Polychronidou

Grobhans

2011

nucleus

Self Cite

View full text Add to dashboard Cite

C hanges in nuclear morphology are observed in diverse developmental processes as well as in pathological conditions. Modification of nuclear membrane and nuclear lamina protein levels results in altered nuclear shapes, as it has been demonstrated in experimental systems ranging from yeast to human cells. The important role of nuclear membrane components in regulating nuclear morphology is additionally highlighted by the abnormally shaped nuclei observed in diseases where nuclear lamina proteins are mutated. Even though the effect of nuclear envelope components on nuclear shape has been thoroughly described, not much is known about the molecular mechanisms that govern these events. In addition to the known role of intermediate filament formation by lamins, here we discuss several mechanisms that might alone or in combination participate in the regulation of nuclear shape observed upon modification of the levels of nuclear membrane and lamina proteins. Based on recent work with the two farnesylated nuclear membrane Drosophila proteins, kugelkern and lamin Dm0, we propose that the direct interaction of farnesylated nuclear membrane proteins with the phospholipid bilayer leads to nuclear envelope deformation. In addition to this mechanism, we suggest that the interaction of nuclear membrane and lamina proteins with cytoskeletal elements and chromatin, and modifications in lipid biosynthesis might also be involved in the formation of abnormally shaped nuclei. Determining nuclear shapeThe role of farnesylated nuclear membrane proteins

show abstract

Developmental Control of Nuclear Size and Shape by kugelkern and kurzkern

Cited by 115 publications

References 29 publications

Emergence of a prestressed eukaryotic nucleus during cellular differentiation and development

Emergence of a prestressed eukaryotic nucleus during cellular differentiation and development

A Green Fluorescent Protein-Based Reporter for Protein Nuclear Import Studies in Drosophila Cells

Determining nuclear shape: The role of farnesylated nuclear membrane proteins

Contact Info

Product

Resources

About