Developmental Changes of Cardiac and Slow Skeletal Muscle Troponin T Expression in Chicken Cardiac and Skeletal Muscles

Identification and analysis of teleost slow muscle troponin T (sTnT) and intronless TnT genes

“…Despite extensive studies in tetrapods, only splice variants, which give rise to different protein isoforms have previously been reported (Wang and Jin, 1997;Bucher et al, 1999;Briggs and Schachat, 1993;Jin et al, 1998a,b;Farza et al, 1998;Yonemura et al, 2000Yonemura et al, , 2002Gahlmann et al, 1987;Samson et al, 1994). Extensive database searches of the human genome in the present study failed to identify additional genes for sTnT.…”

“…All three TnT genes produce multiple isoforms which result from alternative splicing (Gahlmann et al, 1987;Briggs and Schachat, 1993;Samson et al, 1994;Wang and Jin, 1997;Farza et al, 1998;Jin et al, 1998a,b;Bucher et al, 1999;Yonemura et al, 2000Yonemura et al, , 2002Nakada et al, 2002).…”

“…In humans, four sTnT isoforms are generated by alternative splicing of one 5V-exon and an exon located in the central part of the gene (Gahlmann et al, 1987;Samson et al, 1994). In chicken 5 different sTnT isoforms have been identified in adult muscle and also during development (Yonemura et al, 1996(Yonemura et al, , 2000(Yonemura et al, , 2002. In contrast to fast (fTnT) and cardiac TnT (cTnT) genes, no evidence of a foetal specific splice variant of sTnT has been reported in mammals and birds (Gahlmann et al, 1987;Samson et al, 1994;Jin et al, 1998a,b;Perry, 1998;Huang et al, 1999;Yonemura et al, 2002).…”

Regulation of troponin T expression during muscle development in sea breamSparus auratusLinnaeus: the potential role of thyroid hormones

“…As has been previously reported (Campinho et al, 2005) and in contrast to what occurs in tetrapods, sea bream sTnT genes have a coordinated expression pattern during sea bream development (Barton et al, 1999;Briggs and Schachat, 1993;Bucher et al, 1999;Farza et al, 1998;Gahlmann et al, 1987;Huang et al, 1999;Jin et al, 1998a;Jin et al, 2000;Perry, 1998;Samson et al, 1994;Wang and Jin, 1997;Wang et al, 2002;Yonemura et al, 2000;Yonemura et al, 2002). Gene expression of sTnT1sb (Fig.·7) and its splice variants in sea bream, but not sTnT2sb and iTnT (Fig.·6), are affected by T3 treatment.…”

Genomic structure of the chicken slow skeletal muscle troponin T gene

“…Several sTnT cDNA sequences have been reported, which might represent distinct sTnT isoforms in chicken skeletal muscles (Yonemura et al, 2000;Yonemura et al, 2002). To determine whether these transcripts are generated from a single sTnT gene, we performed Southern blot analysis using a 641-bp probe derived from the 3V-region of the sTnT cDNA clone (outlined in Fig.…”