Developmental and cellular functions of the ESCRT machinery in pluricellular organisms

Michelet, Xavier; Djeddi, Abderazak; Legouis, Renaud

doi:10.1042/bc20090145

Cited by 46 publications

(40 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Redundant transmembrane receptors are sorted to intraluminal vesicles predestined for lysosomal degradation after ubiquitination, a post-translational modification that is executed by ESCRT (Babst, 2005;Michelet et al, 2010). On the other hand, there is no compelling evidence that the ESCRT is involved in the sorting of transmembrane receptors to intraluminal vesicles that are predestined to become secreted as exosomes Buschow et al, 2005Buschow et al, , 2009Trajkovic et al, 2008;Simons and Raposo, 2009;Tamai et al, 2010;Bobrie et al, 2011).…”

Section: A Exosomesmentioning

confidence: 97%

Classification, Functions, and Clinical Relevance of Extracellular Vesicles

et al. 2012

View full text Add to dashboard Cite

Section: A Exosomesmentioning

confidence: 97%

Classification, Functions, and Clinical Relevance of Extracellular Vesicles

et al. 2012

View full text Add to dashboard Cite

“…Ultimately, ILVs are degraded after fusion with the lysosome (Gruenberg and Stenmark, 2004;Piper and Katzmann, 2007). Recent studies in the nematode, fly and mammals have shown that mutations in the endosomal sorting complexes required for transport (ESCRT) block endosomal maturation but also lead to an increased number of autophagosomes (Michelet et al, 2009;Michelet et al, 2010;Roudier et al, 2005;Rusten et al, 2007a;Rusten and Stenmark, 2009). The ESCRT machinery is composed of four complexes, ESCRT-0 to ESCRT-III, which are essential for the formation of ILVs in the MVB compartment and for the sorting of ubiquitylated membrane proteins.…”

Section: Introductionmentioning

confidence: 99%

Induction of autophagy in ESCRT mutants is an adaptive response for cell survival in C. elegans

Djeddi

Michelet

Culetto

et al. 2012

Journal of Cell Science

Self Cite

View full text Add to dashboard Cite

SummaryEndosomes and autophagosomes are two vesicular compartments involved in the degradation and recycling of cellular material. They both undergo a maturation process and finally fuse with the lysosome. In mammals, the convergence between endosomes and autophagosomes is a multistep process that can generate intermediate vesicles named amphisomes. Using knockdowns and mutants of the ESCRT machinery (ESCRT-0-ESCRT-III, ATPase VPS-4) and the autophagic pathway (LGG-1, LGG-2, ATG-7, TOR), we analyzed in vivo the functional links between endosomal maturation and autophagy in Caenorhabditis elegans. We report here that, despite a strong heterogeneity of their developmental phenotypes, all ESCRT mutants present an accumulation of abnormal endosomes and autophagosomes. We show that this accumulation of autophagosomes is secondary to the formation of enlarged endosomes and is due to the induction of the autophagic flux and not a blockage of fusion with lysosomes. We demonstrate that the induction of autophagy is not responsible for the lethality of ESCRT mutants but has a protective role on cellular degradation. We also show that increasing the basal level of autophagy reduces the formation of enlarged endosomes in ESCRT mutants. Together, our data indicate that the induction of autophagy is a protective response against the formation of an abnormal vesicular compartment.

show abstract

“…However, this complex regulation of Arabidopsis ESCRT is perhaps not surprising given a number of factors, including: (1) the considerable expansion of many of the ESCRT gene families in plants compared with other eukaryotes, 4,5,15 (2) the sophisticated hierarchical-and stoichiometric-dependent manner in which the various multi-protein ESCRT subcomplexes, their associated regulatory proteins, as well as ubiquitin, appear to operate in concert, [1][2][3]36 (3) the unique and dynamic nature of the plant endosomal pathway overall, 17,18,37 and (4) the ever expanding number of roles that the ESCRT machinery, or at least portions thereof, participates in throughout plant growth and development. 8 Undoubtedly these and other important aspects of the plant ESCRT machinery will be the focus over the coming years of more detailed experimental studies and the results presented here should thus serve as a useful resource for elucidating the molecular mechanisms by which ESCRT functions in plants. …”

mentioning

confidence: 99%

Meta-analysis of the expression profiles of the Arabidopsis ESCRT machinery

Richardson

Mullen²

2011

Plant Signaling & Behavior

View full text Add to dashboard Cite

ESCRT consists of ~20 soluble proteins that are probably best known for their role in the recognition, concentration and packaging of mono-ubiquitinated, membrane-bound proteins within the intralumenal vesicles of endosomal MVBs, leading to the eventual degradation of these membrane proteins upon fusion of the MVB with the lysosome/vacuole. To accomplish this task, ESCRT proteins are sequentially recruited from the cytosol to the surface of the late endosomal membrane via a series of specific protein-protein interactions that results in the assembly of at least four heteromeric protein subcomplexes (ESCRT-0, -I, -II, -III). ESCRT subcomplex disassembly is subsequently regulated, in part, by an AAA-type ATPase referred to as Vps4 (vacuole protein-sorting 4) and its various associated regulatory proteins. [1][2][3] Overall, the breadth of information that exists regarding ESCRT has been garnered primarily from studies with yeast and mammalian cultured cells; however, most of the components of ESCRT are present throughout all eukaryotic taxa, with the exception of ESCRT-0 apparently being absent in plants and other non-opisthokonts (e.g., trypanosomes, Dictyostelium, Chlamydomonas etc.). [4][5][6] Interestingly, components of ESCRT-III are present also in Archaea, 7 supporting the functional importance of ESCRT, or at least portions thereof, among evolutionarily diverse organisms.8 It is also now well established that, in addition to the role of ESCRT in MVB biogenesis and membrane protein degradation via the endocytic pathway, ESCRT participates in a number of other key cellular events, including autophagy, 9 membrane abscission during cytokinesis, 10 retroviral budding, 11 and tombusvirus replication. Keywords: Arabidopsis, (co-)expression, endosome, E-northern, ESCRT, microarray, multivesicular bodyIn light of these new and unexpected functions for ESCRT there is a growing interest in understanding the overall, more global organization and regulation of ESCRT in plants. Toward this end, recent analyses of the ESCRT protein-protein interaction network in Arabidopsis-based mainly on the yeast twohybrid assay-revealed that the majority of these interactions are those that also take place between their counterparts in yeasts and/or mammals, [13][14][15] suggesting that the molecular mechanisms underlying ESCRT function are evolutionarily conserved. On the other hand, the Arabidopsis ESCRT interactome also possesses a number of unique interactions that, when considering the fact that many of the ESCRT components in plants exist as multiple isoforms, 4,5,16 may reflect the functional plasticity and distinct regulation of the plant ESCRT system overall.Here we extend our and other's recent, more global studies of the Arabidopsis ESCRT machinery [13][14][15] by taking advantage of the vast array of publicly-available Arabidopsis gene and protein (peptide) expression data sets and web-based bioinformatics resources to analyze the expression profiles of many of the Arabidopsis ESCRT components across a variety of differen...

show abstract

Developmental and cellular functions of the ESCRT machinery in pluricellular organisms

Cited by 46 publications

References 90 publications

Classification, Functions, and Clinical Relevance of Extracellular Vesicles

Classification, Functions, and Clinical Relevance of Extracellular Vesicles

Induction of autophagy in ESCRT mutants is an adaptive response for cell survival in C. elegans

Meta-analysis of the expression profiles of the Arabidopsis ESCRT machinery

Contact Info

Product

Resources

About