Development, optimization, and invitro evaluation of novel fast dissolving oral films (FDOF's) of Uncaria tomentosa extract to treat osteoarthritis

Sowjanya, J. Naga; Rao, P.Raja

doi:10.1016/j.heliyon.2023.e14292

Cited by 9 publications

(4 citation statements)

References 19 publications

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“…The maximum concentration of drug in plasma (C max ), duration to peak plasma concentration (T max ), biological half-life of the drug (t1/2), area under plasma concentration versus time plot (AUC), and mean residence time (MRT) were all determined with the help of PK summit solutions software USA. 21,22 The results were given in Table 6 and depicted in Figure 16.…”

Section: In-vivo Pharmacokinetic Studies Of Cevimeline Hcl Fast Disso...mentioning

confidence: 99%

In-vitro and In-vivo Evaluation of Cevimeline HCl Fast Dissolving Films

Akash,

Shailaja

2023

IJDDT

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The primary aim and objective of the present work was to formulate the cevimeline HCl fast-dissolving films and assess the films, a pharmaceutical compound utilized for the management of xerostomia symptoms linked to Sjogren’s disease. Fast-dissolving films of cevimeline HCl were formulated by utilizing the solvent casting technique and the sodium carboxy methyl cellulose, HPMC E5, polyvinyl alcohol, and polyvinyl pyrrolidone were used as film-forming agents. Additionally, polyethylene glycol 600 and sodium starch glycolate were used as a super disintegrant and plasticizers. The formulation contains citric acid and stevia powder, which serve the purpose of stimulating saliva and providing sweetness, respectively. This study focused mainly on the development and assessment of cevimeline HCl fast-dissolving films, specifically examining parameters such as film thickness, folding resistance, drug content, air bubble entrapment, and film curling. The obtained results were found to be consistent with the preset ranges established for these parameters. The drug release from the fast-dissolving films formulated with PVP K30 was 99.91% released within 5 minutes timeframe. The results indicates that the films prepared with PVP K30 show enhanced solubility, rate of dissolution, flexibility, and tensile strength in comparison to the films formulated with sodium carboxy methyl cellulose, HPMC E5 and Polyvinyl alcohol. Fourier transform infrared (FTIR) and differential scanning calorimetry (DSC) studies were done to characterize the pure drug, polymers and to C11 the optimized formulation. These findings indicated, no observed incompatibilities among the drug and polymers utilized in this study. Moreover, in-vivo investigations were conducted on optimized formulation C11, which demonstrated its notable stability.

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Section: In-vivo Pharmacokinetic Studies Of Cevimeline Hcl Fast Disso...mentioning

confidence: 99%

In-vitro and In-vivo Evaluation of Cevimeline HCl Fast Dissolving Films

Akash,

Shailaja

2023

IJDDT

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“…The feasibility of developing oral films as a delivery system for compounds with different biological activities has been studied, such as for the treatment of osteoarthritis [1], rosuvastatin bioavailability [2], hypertension [3], oral cancer [4], and anti-inflammatory activity [5], among others. Commercially, some pharmaceutical companies are already working with orodispersible films such as Sympazan ® (Otter Pharmaceuticals, Lake Forest, CA, USA), NiQuitine Strips ® (GlaxoSmithKline plc, London, UK), Gas-X Thin Strips ® (Novartis AG, Basel, Switzerland), and Listerine Pocket Packs ® (Johnson & Johnson, New Brunswick, NJ, USA).…”

Section: Introductionmentioning

confidence: 99%

“…Alternatives for the administration of the natural active compound have been explored, such as gels [29][30][31][32], ointments [33][34][35], and mainly orally disintegrating films [1,5,36]. Generally, oral films are developed from natural polymers [1,3,[36][37][38][39], and their characteristics, such as disintegration time, stability of the active compounds, and biological activities, among others, are determined since they can be affected by several factors.…”

Section: Introductionmentioning

confidence: 99%

Oral Films Printed with Green Propolis Ethanolic Extract

Remedio,

Garcia,

Rochetti

et al. 2024

Polymers

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Oral film (OF) research has intensified due to the effortless administration and advantages related to absorption in systemic circulation. Chitosan is one of the polymers widely used in the production of OFs; however, studies evaluating the maintenance of the active principles’ activity are incipient. Propolis has been widely used as an active compound due to its different actions. Printing techniques to incorporate propolis in OFs prove to be efficient. The objective of the present study is to develop and characterize oral films based on chitosan and propolis using printing techniques and to evaluate the main activities of the extract incorporated into the polymeric matrix. The OFs were characterized in relation to the structure using scanning and atomic force electron microscopy; the mechanical properties, disintegration time, wettability, and stability of antioxidant activity were evaluated. The ethanolic extract of green propolis (GPEE) concentration influenced the properties of the OFs. The stability (phenolic compounds and antioxidant activity) was reduced in the first 20 days, and after this period, it remained constant.

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“…On day 10, MIA (Sigma-Aldrich, MO, USA) was injected in a 1 mL syringe at a dose of 50 µL (60 mg/mL) in the right knee joint to induce OA. After injection of MIA, each experimental group was administered orally with either saline, 150 or 300 mg/kg F5, and 100 mg/kg Celecoxib (Kekule Pharma limited, Hyderabad) once daily for 5 weeks[25][26][27].…”

mentioning

confidence: 99%

In Vitro and In vivo approaches to evaluate Uncaria tomentosa bark extract loaded FDOFs on Osteoarthritis models

Sowjanya

Rao

2023

Preprint

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Osteoarthritis is one the leading health concerns worldwide affecting two third million with no proper treatment ensured to restore the normal function and completely relieving the joint pain. Oral fast dissolving films have promising action and targeted delivery with high drug loading capacity. The present investigation involves the study the invitro and invivo activity of developed Oral fast dissolving films of U. tomentosa bark extract with optimised F5 and F13 formulations. For invitro evaluation a three dimensional OA model was prepared with first passage chrondrocytes grown on trypsin EDTA media in 1: 3 ratio. The OA agarose model was prepared with C20A4 chondrocytes on agarose gel (25 ± 5oC) in phospahate buffer solution. Cultivation of chrondrocytes was done with 1 mL of RPMI-1640 (10% FBS) which was added with 20% (IL-1β) solution on third day of incubation and media was replaced time to time. The incubated cell line with 20,000 cells/well in 96-well plates were treated with 5 µL of 0.5% MTT reagent on fifth day of incubation and absorbance was measured at 570 nm. The effects were studied for 7, 13, 27, 35 days for the study effects of FDOFs on the cell lines were (Control, IL-1β, F5, and F13 treated IL-1β injected types). The chondrocytes in agarose constructs cultured only in media (RPMI-FBS) without IL-1b, served as control. The GAG, HYP and DNA quantitation analyses along with DNA content assay were performed to study the arthritic effect of optimized FDOF’s i.e F5. For invivo studies Monoiodoacetate (MIA) induced arthritis models which is well established to understand weight bearing and response to tactile stimuli though the ongoing procedure is not known. The invivo protocol was performed in seven week old male wistar rats with negative control of MIA and positive control as Celecoxib. The assessment of pain and thickness of the knee were estimated to be indicators of osteoarthritic potential. The study results revealed the F5 formulation has efficacy on the OA models which need a clinical investigation in humans.

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Development, optimization, and invitro evaluation of novel fast dissolving oral films (FDOF's) of Uncaria tomentosa extract to treat osteoarthritis

Cited by 9 publications

References 19 publications

In-vitro and In-vivo Evaluation of Cevimeline HCl Fast Dissolving Films

In-vitro and In-vivo Evaluation of Cevimeline HCl Fast Dissolving Films

Oral Films Printed with Green Propolis Ethanolic Extract

In Vitro and In vivo approaches to evaluate Uncaria tomentosa bark extract loaded FDOFs on Osteoarthritis models

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