Development of sequential-co-culture system (Pichia stipitis and Zymomonas mobilis) for bioethanol production from Kans grass biomass

In situ detoxification of dry dilute acid pretreated corn stover by co-culture of xylose-utilizing and inhibitor-tolerant Saccharomyces cerevisiae increases ethanol production

“…The highest production yield Yp/s (0.453 g ethanol/g sugar) and maximum ethanol concentration Cm (57.8 g/L) was achieved by the fed-batch fermentation of inorganic acid hydrolyzed Kans grass biomass through the co-culture system of Z. mobilis and P. stipitis (Singh et al, 2014). Compared with fermentation of un-detoxification hydrolysate, the Yp/s and Cm was higher in the fermentation of detoxification hydrolysate.…”

Lignocellulosic bioethanol production: prospects of emerging membrane technologies to improve the process – a critical review

“…Chandel et al (2011) achieved a maximum 15 ± 0.92 g/l bioethanol production from Saccharum spontaneum implementing cocultures of P. stipitis and thermotolerant S. cerevisiae. In a similar experimental investigation with S. spontaneum, Singh et al (2014) developed a sequential coculture system with P. stipitis and Z. mobilis for better utilization of total sugars. Through the successful applications of metabolic engineering, the E. coli strain was developed with the ability to ferment a wide variety of sugars with no additional requirements (Doelle et al 1989).…”

“…Among the existing process of the modified SSF approach, simultaneous saccharification and cofermentation (SSCF) has been most feasible approach to convert both hexose and pentose sugars to increased concentration of lignocellulosic bioethanol. For the bioconversion of both types of pentose and hexose sugars in SSCF processes, ideal coculture fermentation with combinations of suitable microorganisms, which do not effect each other's metabolic activities, are mainly required (Chandel et al 2011, Singh et al 2014. Singh et al (2014) performed sequential coculture fermentation system with P. stipitis and Z. mobilis in a 7 l bioreactor and the average ethanol yield and productivity were 0.453 g/g and 1.580 g/l h from kans grass-based substrate.…”

“…For the bioconversion of both types of pentose and hexose sugars in SSCF processes, ideal coculture fermentation with combinations of suitable microorganisms, which do not effect each other's metabolic activities, are mainly required (Chandel et al 2011, Singh et al 2014. Singh et al (2014) performed sequential coculture fermentation system with P. stipitis and Z. mobilis in a 7 l bioreactor and the average ethanol yield and productivity were 0.453 g/g and 1.580 g/l h from kans grass-based substrate. Chandel et al (2011) made efforts toward coculture fermentation system with P. stipitis and thermotolerent S. cerevisiae with an appropriate ratio.…”

Engineered microbial consortium for the efficient conversion of biomass to biofuels: A preliminary study

“…One strain required adenine and overproduced lysine while the other strain required lysine and overproduced adenine. Singh et al (2014) co-cultured Pichia stipitis and Z. mobilis for bioethanol production from kans grass biomass with significant yields and Zhang et al (2016a) employed C. cellulolyticum and hydrogen fermentation bacteria for enhanced biohydrogen production from corn stover with significant differences seen in the metabolites of the co-culture system over the mono-cultures. Other reports of successful bio-catalysis based on microbial consortia have equally been reported (Fu et al, 2009;He et al, 2011;Ho et al, 2011;Li et al, 2011;Quinn et al, 2016;Reddy and Basappa, 1996;Yaun et al, 2016;Zhang et al, 2016b;Zhong et al, 2016).…”