2010
DOI: 10.1515/sg-2010-0035
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Abstract: The development of molecular markers unambiguously distinguishing groups at different taxonomic levels has numerous forensic applications. The identification of tropical timber is of particular interest in this context. We describe the development of SCAR (Sequence Characterized Amplified Region) markers for forensic applications taking the example of two closely related species of the tropical tree family Shorea (Dipterocarpaceae). Two AFLP (Amplified Fragment Length Polymorphism) fragments have been describe… Show more

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Cited by 6 publications
(9 citation statements)
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“…As a reference, S. parvifolia populations were sampled from 10 natural forests in Indonesia: four populations in Sumatra and six populations in Borneo. Shorea parvifolia and S. leprosula have very similar wood anatomies (Bosman and Baas 1996), but can be distinguished at locus-specific DNA markers (Nuroniah et al 2010).…”
Section: Methodsmentioning
confidence: 99%
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“…As a reference, S. parvifolia populations were sampled from 10 natural forests in Indonesia: four populations in Sumatra and six populations in Borneo. Shorea parvifolia and S. leprosula have very similar wood anatomies (Bosman and Baas 1996), but can be distinguished at locus-specific DNA markers (Nuroniah et al 2010).…”
Section: Methodsmentioning
confidence: 99%
“…Two different selective amplifications were prepared: (1) using the MseI+4 primer and EcoRI primer labeled with the fluorescent dye Cy-5 for gel-extraction from a 7% polyacrylamide (PAA) gel and (2) using the MseI+4 primer and EcoRI primer labeled with the fluorescent dye 6-FAM for comparison with the original AFLP fingerprint after capillary electrophoresis. AFLP fragments were excised from 7% PAA gels following the protocol described in Nuroniah et al (2010). Two microliters of the purified AFLP fragments were used for PCR.…”
Section: Methodsmentioning
confidence: 99%
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