Development of photo-crosslinkable platelet lysate-based hydrogels for 3D printing and tissue engineering

Min, Sung Jun; Lee, Jae Seo; Nah, Haram; Kim, Seung Hyeon; Moon, Ho Jin; Reis, Rui L.; Kwon, Il Keun; Heo, Dong Nyoung

doi:10.1088/1758-5090/ac1993

Cited by 12 publications

(12 citation statements)

References 60 publications

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“…Additionally, the biological performance can be improved by allowing slower degradation kinetics and/or controlled release of drugs/growth factors. This technique can be easily combined with other ink engineering and/or crosslinking strategies to deposit the low viscosity materials with mechanical and biological gradients [119]. A similar technique relies on the use of sacrificial materials that are printed providing structural support to the materials of interest, but that can be removed after their gelification, resulting in a construct with tunable porosity [120,121].…”

Section: Alternative Approaches For 3d Printingmentioning

confidence: 99%

“…This ionic interaction can be pursued by co-extrusion of alginate and calcium chloride [176], extrusion of alginate-based inks into calcium chloride support baths/layers [177], or even by pre-gelifying alginate before extrusion, requiring low concentrations of ionic crosslinker [178]. Many researchers combine other natural-based materials with alginate to obtain an ink comprising alginates' physical and rheological proprieties along with the biological relevance of the materials of interest [119,179,180]. This technique may also be very advantageous for the inclusion of cationic nanoparticles into a negatively charged matrix.…”

Section: Enzyme-basedmentioning

confidence: 99%

“…Indeed, these natural-origin matrices need to be modified or mixed with other synthetic/natural polymers to match the ink's desirable proprieties (section 2.1), especially for extrusion printing. There are several studies following the rationale of having synthetic polymeric support for depositing the natural inks in-between the synthetic filaments (figure 5(a)), providing mechanical support while the matrices undergo reticulation [118,119,242,243]. Other authors mixed PLGA with tissue-specific ECM and HA to act as an ink binder for extrusion printing (figure 5(b)), which demonstrated being a valuable scaffold for spine fusion [239][240][241].…”

Section: State-of-the-artmentioning

confidence: 99%

“…Interestingly, amongst the published studies, this is the most adapted methodology for developing inks based on blood-derived components (table 2). Due to their almost liquid nature, this modification technique allows for the obtention of highly stable and mechanically robust hydrogels, with demonstrated extrudability, injectability, and tunable stiffnesses [119,238], being a promising strategy for the obtention of biocompatible materials that enable proliferation and differentiation of stem cells [119,237]. Figure 6 schematizes different methods of using gelMA in combination with human-derived biomaterials such as PL (figure 6(a)) and PRP (figure 6(b)).…”

Section: State-of-the-artmentioning

confidence: 99%

“…(c) By printing PLMA within a PCL framework, the printed filaments can hold their shape and are further photoreticulated during the extrusion process. The authors demonstrated the feasibility of this methodology to print different cell types, including HUVECs for improved vascularization [119].…”

Section: Clinical Translation: Current Limitations and Future Perspec...mentioning

confidence: 99%

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Biomaterials of human source for 3D printing strategies

et al. 2023

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Three-dimensional printing has risen in recent years as a promising approach that fast-tracked the biofabrication of tissue engineering constructs that most resemble utopian tissue/organ replacements for precision medicine. Additionally, by using human-sourced biomaterials engineered towards optimal rheological proprieties of extrudable inks, the best possible scaffolds can be created. These can encompass native structure and function with a low risk of rejection, enhancing overall clinical outcomes; and even be further optimized by engaging in information- and computer-driven design workflows. This paper provides an overview of the current efforts in achieving ink’s necessary rheological and print performance proprieties towards biofabrication from human-derived biomaterials. The most notable step for arranging such characteristics to make biomaterials inks are the employed crosslinking strategies, for which examples are discussed. Lastly, this paper illuminates the state-of-the-art of the most recent literature on already used human-sourced inks; with a final emphasis on future perspectives on the field.

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Section: Alternative Approaches For 3d Printingmentioning

confidence: 99%

Section: Enzyme-basedmentioning

confidence: 99%

Section: State-of-the-artmentioning

confidence: 99%

Section: State-of-the-artmentioning

confidence: 99%

Section: Clinical Translation: Current Limitations and Future Perspec...mentioning

confidence: 99%

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Biomaterials of human source for 3D printing strategies

et al. 2023

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Embedding Bioprinting of Low Viscous, Photopolymerizable Blood‐Based Bioinks in a Crystal Self‐Healing Transparent Supporting Bath

Caiado Decarli,

Ferreira,

Sobreiro‐Almeida

et al. 2024

Small Methods

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Protein‐based hydrogels have great potential to be used as bioinks for biofabrication‐driven tissue regeneration strategies due to their innate bioactivity. Nevertheless, their use as bioinks in conventional 3D bioprinting is impaired due to their intrinsic low viscosity. Using embedding bioprinting, a liquid bioink is printed within a support that physically holds the patterned filament. Inspired by the recognized microencapsulation technique complex coacervation, crystal self‐healing embedding bioprinting (CLADDING) is introduced based on a highly transparent crystal supporting bath. The suitability of distinct classes of gelatins is evaluated (i.e., molecular weight distribution, isoelectric point, and ionic content), as well as the formation of gelatin‐gum arabic microparticles as a function of pH, temperature, solvent, and mass ratios. Characterizing and controlling this parametric window resulted in high yields of support bath with ideal self‐healing properties for interaction with protein‐based bioinks. This support bath achieved transparency, which boosted light permeation within the bath. Bioprinted constructs fully composed of platelet lysates encapsulating a co‐culture of human mesenchymal stromal cells and endothelial cells are obtained, demonstrating a high‐dense cellular network with excellent cell viability and stability over a month. CLADDING broadens the spectrum of photocrosslinkable materials with extremely low viscosity that can now be bioprinted with sensitive cells without any additional support.

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