Development of Pepper Transgenic Plants via Agrobacterium and Biolistic Transformation

Nianiou, I.; Karavangeli, Margarita; Zambounis, Antonios; Tsaftaris, Athanasios

doi:10.17660/actahortic.2002.579.10

Cited by 7 publications

(2 citation statements)

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“…In the last decade, Agrobacterium tumefaciens-mediated stable genetic transformation has been successfully performed with several members of the Solanaceae family (Ochoa-Alejo and Ramirez-Malagon, 2001;Nianiou et al 2002). However, the genus Capsicum has remained largely recalcitrant to stable transformation and as a result only a low frequency and reproducibility (0.5-1% success rate) of transformation has been successfully achieved (Manoharan et al 1998;Kim et al 2001b;Li et al 2003;Lee et al 2004).…”

mentioning

confidence: 99%

Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants

Arcos-Ortega

Chan-Kuuk

González-Kantún

et al. 2010

Electron. J. Biotechnol.

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mentioning

confidence: 99%

Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants

Arcos-Ortega

Chan-Kuuk

González-Kantún

et al. 2010

Electron. J. Biotechnol.

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“…Similarly, hypocotyl explant resulted in the highest transformation efficiency compared to leaf and cotyledonary leaf explants in perilla (Lee et al, 2005). Hypocotyl explants were successfully used in Agrobacterium-mediated transformation in chilli (Nianiou et al, 2000) and cauliflower (Chakrabarty et al, 2002). In conclusion, it is stated that hypocotyl explant is better as compared to cotyledonary leaf or leaf tissue for transformation studies in brinjal.…”

Section: Effect Of Explant On Transformation and Morphogenetic Respon...mentioning

confidence: 81%

Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota

Prakash,

Deepali,

Asokan

et al. 2007

J. Hortic. Sci.

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Effects of growth regulators and type of explants on transformation and in vitro morphogenetic responses of brinjal cv. Manjarigota were studied. Both hypocotyl and cotyledonary explants showed marked influence on in vitro morphogenetic responses after Agrobacterium co-cultivation. Hypocotyl explants showed callus initiation and regeneration responses earlier than cotyledonary leaves. Hypocotyl explants were found to be better than cotyledonary leaf explants in regenerating shoots after Agrobacterium co-cultivation. There was delay and reduction in both callus and regeneration responses in Agrobacterium co-cultivated explants. Hypocotyl explants showed the highest regeneration response on MS medium containing 2 μM BAP and 0.05 μM NAA while cotyledonary leaves did not show regeneration response after Agrobacterium co-cultivation. However, they showed green buds on MS medium containing 10 μM BAP and 1 μM NAA, which could not differentiate into shoots. Overall, hypocotyl explants were found better in regenerating shoots after Agrobacterium co-cultivation.

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Recent Trends in Sweet Pepper Breeding

Sharma

Srivastava

Mangal

2020

Accelerated Plant Breeding, Volume 2

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Development of Pepper Transgenic Plants via Agrobacterium and Biolistic Transformation

Cited by 7 publications

References 0 publications

Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants

Agrobacterium tumefaciens-transient genetic transformation of Habanero pepper (Capsicum chinense Jacq.) leaf explants

Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota

Recent Trends in Sweet Pepper Breeding

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