Development of optical immunosensors for detection of proteins in serum

Kyprianou, Dimitris; Chianella, Iva; Guerreiro, António; Piletska, Elena; Piletsky, Sergey A.

doi:10.1016/j.talanta.2012.10.042

Cited by 19 publications

(10 citation statements)

References 31 publications

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“…After conditioning, the SPR chamber was supplemented with 100 μL of buffer and conditioned for 30 min. GST-His 6 -JAK2 probe was diluted with buffer to 1x10 -6 M, briefly vortexed and injected on a modified SPR gold disc (6,12,26, and 44 L) in order to obtain the following concentrations: 50, 100, 200, 300 nM. After 2h incubation, GST-His 6 -JAK2 solution was rinsed off with 2 mL of the buffer and the value of the SPR angle shift was recorded 20 min after the end wash.…”

Section: Immobilization Of Gst-his 6 -Jak2mentioning

confidence: 99%

“…Here, we present the work on biosensors based on the principle of surface plasmon resonance (SPR), which have emerged among the most widely used label free detection tools for the study of biomolecular interactions such as protein-protein [9,10], antibody-antigen [11,12] and receptor-ligand [13,14]. SPR offers the ability for direct measuring of interactions in real time, which allows the quantitative determination of kinetic and thermodynamic parameters [15].…”

Section: Introductionmentioning

confidence: 99%

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Immobilization of His-tagged kinase JAK2 onto the surface of a plasmon resonance gold disc modified with different copper (II) complexes

Kurzątkowska¹,

Mielecki²,

Grzelak³

et al. 2014

Talanta

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New surface plasmon resonance (SPR) sensing platform swhich consists of copper (II) complexes of a pentetic acid thiol ligand (DPTA-Cu(II)) and of a thiol derivative of dipyrromethene (DPM-Cu(II) created on the surface of gold SPR disc were applied to oriented immobilization of His-tagged Janus kinase 2 (GST-His 6 -JAK2). This method is based on the covalent bond formation between histidine from a His-tag chain of a protein and Cu(II) centres from the complexes. The kinetic and thermodynamic parameters of the oriented immobilization of GST-His 6 -JAK2 protein to DPTA-Cu(II) and DPM-Cu(II) complexes attached to the Au surface of a SPR disc were discussed.

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Section: Immobilization Of Gst-his 6 -Jak2mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immobilization of His-tagged kinase JAK2 onto the surface of a plasmon resonance gold disc modified with different copper (II) complexes

Kurzątkowska¹,

Mielecki²,

Grzelak³

et al. 2014

Talanta

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show abstract

“…Although current PC biosensor methodologies require a laboratory setting, a portable optical interface capable of converting a smartphone into a detection instrument was recently designed for the PC biosensor (Gallegos et al, 2013). Nevertheless, the most significant challenge continues to be the accurate and sensitive detection of biomarkers in complex matrixes (Marks, 2002;Kyprianou et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…serum and whole blood) containing variable amounts of interfering proteins are the primary cause of non-specific binding and poor signal-to-noise responses (Kyprianou et al, 2013), which results in unreliable data. These can lead to false-negative or false-positive clinical results that instead increase healthcare costs and burden the health of patients.…”

Section: Introductionmentioning

confidence: 99%

Enhanced sandwich immunoassay using antibody-functionalized magnetic iron-oxide nanoparticles for extraction and detection of soluble transferrin receptor on a photonic crystal biosensor

Peterson

Chen

Cunningham

et al. 2015

Biosensors and Bioelectronics

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Iron deficiency anemia (IDA) has detrimental effects on individuals and societies worldwide. A standard sandwich assay (SA) for the detection of soluble transferrin receptor (sTfR), a biomarker of IDA, on a photonic crystal (PC) biosensor was established, but it was susceptible to non-specific signals from complex matrixes. In this study, iron-oxide nanoparticles (fAb-IONs) were used as magnetic immuno-probes to bind sTfR and minimize non-specific signals, while enhancing detection on the PC biosensor. This inverse sandwich assay (IA) method completely bound sTfR with low variability (<4% RSD) in buffer and allowed for its accurate and precise detection in sera (Liquichek™ control sera) on the PC biosensor using two certified ELISAs as reference methods. A linear dose-response curve was elicited at the fAb-IONs concentration in which the theoretical binding ratio (sTfR:fAb-IONs) was calculated to be <1 on the IA. The LoDs for sTfR in the SA and IA were similar (P>0.05) at 14 and 21 μg/mL, respectively. The inherent imprecision of the IA and reference ELISAs was σ(δ)=0.45 µg/mL and the mean biases for Liquichek™ 1, 2 and 3 were 0.18, 0.19 and -0.04 µg/mL, respectively. Whereas the inherent imprecision of the SA and reference ELISAs was σ(δ)=0.52 µg/mL and the biases for Liquichek™ 1, 2 and 3 were 0.66, 0.14 and -0.67 µg/mL, respectively. Thus, unlike the SA, the IA method measures sTfR with the same bias as the reference ELISAs. Combined magnetic separation and detection of nutrition biomarkers on PC biosensors represents a facile method for their accurate and reliable quantification in complex matrixes.

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“…SPR and SPRi immunosensors are particularly interesting to detect biomolecular interactions in real time with complex biological media [7][8][9]. This is due to their ability to detect biomolecular interactions in a label-free manner [10] and to their high sensitivity and "real-time monitoring" capabilities [11].…”

mentioning

confidence: 99%

Biochip technology applied to an automated ABO compatibility test at the patient bedside

Charrière¹,

Rouleau²,

Gaiffe³

et al. 2015

Sensors and Actuators B: Chemical

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Development of optical immunosensors for detection of proteins in serum

Cited by 19 publications

References 31 publications

Immobilization of His-tagged kinase JAK2 onto the surface of a plasmon resonance gold disc modified with different copper (II) complexes

Immobilization of His-tagged kinase JAK2 onto the surface of a plasmon resonance gold disc modified with different copper (II) complexes

Enhanced sandwich immunoassay using antibody-functionalized magnetic iron-oxide nanoparticles for extraction and detection of soluble transferrin receptor on a photonic crystal biosensor

Biochip technology applied to an automated ABO compatibility test at the patient bedside

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