Development of molecular markers for genetic and physical mapping of the <i>PcDw</i> locus in pear (<i>Pyrus communis</i> L.)

Wang, C. H.; Li, W.; Hou, D. L.; Bai, Meihan

doi:10.1080/14620316.2016.1155319

Cited by 8 publications

(7 citation statements)

References 30 publications

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“…In our previous study, we found that the dwarf growth habit, initially discovered in a French cultivar of Pyrus communis ‘Le Nain Vert’, exhibited monogenic inheritance determined by a dominant gene PcDw , and we narrowed the range of the PcDw gene within scaffold00074 in the pear genome 10 . A total of 49 genes were identified in this region based on the pear genome sequence.…”

Section: Discussionmentioning

confidence: 96%

“…We crossed ‘Aihuali’ with the primary cultivar ‘Chili’ ( Pyrus bretschneideri ), and the chi-square analysis showed that the dwarf and standard character fitted a 1:1 ratio, which was controlled by a single dominant gene designated PcDw 9 . Subsequently, we narrowed the range of the PcDw gene within scaffold00074 by simple sequence repeat and single-nucleotide polymorphism markers 10 . Recently, several candidate genes were revealed by comparative transcriptome analysis 11 .…”

Section: Introductionmentioning

confidence: 99%

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Deletion in the Promoter of PcPIN-L Affects the Polar Auxin Transport in Dwarf Pear (Pyrus communis L.)

Zheng

Xiao

Wang

2019

Sci Rep

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Dwarf cultivars or dwarfing rootstocks enable high-density planting and are therefore highly desirable in modern pear production. Previously, we found that the dwarf growth habit of pear is controlled by a single dominant gene PcDw. In this study, PcPIN-L (PCP021016) was cloned from dwarf-type and standard-type pears. PcPIN-L expression was significantly lower in the dwarf-type pears than in standard-type pears, which was caused by the CT repeat deletion in the promoter of dwarf-type pears. PcPIN-L overexpression in tobacco plants enhanced the growth of the stems and the roots. Notably, the indole acetic acid (IAA) content decreased in the shoot tips and increased in the stems of transgenic lines compared with wild type, which is consistent with the greater IAA content in the shoot tips and lower IAA content in the stems of dwarf-type pears than in standard-type pears. The CT repeat deletion in the promoter that causes a decrease in promoter activity is associated with lower PcPIN-L expression in the dwarf-type pears, which might limit the polar auxin transport and in turn result in the dwarf phenotype. Taken together, the results provide a novel dwarfing molecular mechanism in perennial woody plants.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Deletion in the Promoter of PcPIN-L Affects the Polar Auxin Transport in Dwarf Pear (Pyrus communis L.)

Zheng

Xiao

Wang

2019

Sci Rep

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“…By identifying marker loci that are tightly linked to and that flank a desired gene locus, the position of the gene can be narrowed down to a small region in the chromosome or contig. Using this strategy, some candidates for the pear PcDw gene have been identified from the most probable region ( Wang et al, 2011 ; Wang C. H. et al, 2016 ). In the present study, we predicted eight genes as candidates for PpRus in the region between PpSSRa19 and PpSSRa60 ( Figure 3D and Supplementary Table 4 ).…”

Section: Discussionmentioning

confidence: 99%

“…In many horticultural fruit species, marker-assisted selection (MAS) for major agricultural traits has been developed. For example, molecular markers associated with pear scab resistance, harvest time, and dwarf tree architecture have been developed and applied to pear breeding programs ( Terakami et al, 2006 ; Yamamoto et al, 2014 ; Wang C. H. et al, 2016 ). In our previous study, the gene that determines fruit russet skin was localized to linkage group 8 (LG8) of the pear consensus genetic map using simple sequence repeat (SSR) markers ( Song et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

PpMYB36 Encodes a MYB-Type Transcription Factor That Is Involved in Russet Skin Coloration in Pear (Pyrus pyrifolia)

Wang

et al. 2021

Front. Plant Sci.

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Fruit color is one of the most important external qualities of pear (Pyrus pyrifolia) fruits. However, the mechanisms that control russet skin coloration in pear have not been well characterized. Here, we explored the molecular mechanisms that determine the russet skin trait in pear using the F1 population derived from a cross between russet skin (‘Niitaka’) and non-russet skin (‘Dangshansu’) cultivars. Pigment measurements indicated that the lignin content in the skin of the russet pear fruits was greater than that in the non-russet pear skin. Genetic analysis revealed that the phenotype of the russet skin pear is associated with an allele of the PpRus gene. Using bulked segregant analysis combined with the genome sequencing (BSA-seq), we identified two simple sequence repeat (SSR) marker loci linked with the russet-colored skin trait in pear. Linkage analysis showed that the PpRus locus maps to the scaffold NW_008988489.1: 53297-211921 on chromosome 8 in the pear genome. In the mapped region, the expression level of LOC103929640 was significantly increased in the russet skin pear and showed a correlation with the increase of lignin content during the ripening period. Genotyping results demonstrated that LOC103929640 encoding the transcription factor MYB36 is the causal gene for the russet skin trait in pear. Particularly, a W-box insertion at the PpMYB36 promoter of russet skin pears is essential for PpMYB36-mediated regulation of lignin accumulation and russet coloration in pear. Overall, these results show that PpMYB36 is involved in the regulation of russet skin trait in pear.

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“…We previously reported that the dwarf growth habit, initially discovered in the French pear cultivar ‘Le Nain Vert’, exhibits monogenic inheritance determined by the dominant gene PcDw (Wang et al., 2011). Subsequently, we used simple sequence repeat and single nucleotide polymorphism markers to locate and fine‐map the PcDw locus within scaffold00074 in the pear genome (Wang et al., 2016). We then determined transcript levels of genes within this chromosomal region via comparative transcriptome analysis between regular and dwarf pears, leading to the identification of a gene (PCP021014.1) encoding an arabinogalactan protein (AGP) 7‐like with high expression in dwarf pears plants as a key candidate gene for PcDw (Xiao et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

A mutation in the promoter of the arabinogalactan protein 7‐like gene PcAGP7‐1 affects cell morphogenesis and brassinolide content in pear (Pyrus communis L.) stems

Zheng

et al. 2021

The Plant Journal

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SUMMARY Dwarfing rootstocks and dwarf cultivars are urgently needed for modern pear cultivation. However, germplasm resources for dwarfing pear are limited, and the underlying mechanisms remain unclear. We previously showed that dwarfism in pear is controlled by the single dominant gene PcDw (Dwarf). We report here that the expression of PcAGP7‐1 (ARABINOGALACTAN PROTEIN 7‐1), a key candidate gene for PcDw, is significantly higher in dwarf‐type pear plants because of a mutation in an E‐box in the promoter. Electrophoretic mobility shift assays and transient infiltration showed that the transcription factors PcBZR1 and PcBZR2 could directly bind to the E‐box of the PcAGP7‐1 promoter and repress transcription. Moreover, transgenic pear lines overexpressing PcAGP7‐1 exhibited obvious dwarf phenotypes, whereas RNA interference pear lines for PcAGP7‐1 were taller than controls. PcAGP7‐1 overexpression also enhanced cell wall thickness, affected cell morphogenesis, and reduced brassinolide (BL) content, which inhibited BR signaling via a negative feedback loop, resulting in further dwarfing. Overall, we identified a dwarfing mechanism in perennial woody plants involving the BL‐BZR/BES‐AGP‐BL regulatory module. Our findings provide insight into the molecular mechanism of plant dwarfism and suggest strategies for the molecular breeding of dwarf pear cultivars.

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Development of molecular markers for genetic and physical mapping of the PcDw locus in pear (Pyrus communis L.)

Cited by 8 publications

References 30 publications

Deletion in the Promoter of PcPIN-L Affects the Polar Auxin Transport in Dwarf Pear (Pyrus communis L.)

Deletion in the Promoter of PcPIN-L Affects the Polar Auxin Transport in Dwarf Pear (Pyrus communis L.)

PpMYB36 Encodes a MYB-Type Transcription Factor That Is Involved in Russet Skin Coloration in Pear (Pyrus pyrifolia)

A mutation in the promoter of the arabinogalactan protein 7‐like gene PcAGP7‐1 affects cell morphogenesis and brassinolide content in pear (Pyrus communis L.) stems

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