Development of microsatellite primers for Melanoxylon brauna (Fabaceae): an endangered and endemic tree from the Brazilian Atlantic Forest

Borges, Daniela B.; Mariano‐Neto, Eduardo; Gaiotto, Fernanda Amato

doi:10.1007/s12686-014-0288-3

Cited by 4 publications

(4 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The four subpopulations evaluated with the nSSR loci showed genetic diversity (H E and H O ) estimates similar to those found in rare and overexploited palm and tree species from the Atlantic Forest in southern Bahia, such as Euterpe edulis Mart. (H E = 0.64 and H O = 0.58) and Melanoxylon brauna Schott (H E = 0.57 and H O = 0.53) (Borges et al , 2014; Santos et al , 2015). However, these diversity indices are low compared to those of other more abundant species in the same environment, such as Licania hypoleuca Benth.…”

Section: Discussionmentioning

confidence: 98%

Gene pool sharing and genetic bottleneck effects in subpopulations of Eschweilera ovata (Cambess.) Mart. ex Miers (Lecythidaceae) in the Atlantic Forest of southern Bahia, Brazil

et al. 2019

Self Cite

View full text Add to dashboard Cite

Forest loss and fragmentation are the main threats to the maintenance of the Atlantic Forest, an important global biodiversity hotspot. Because of the current critical level of deforestation, ecological corridors are needed to facilitate species dispersion and gene flow among fragments. This study was conducted to investigate the genetic variability and gene pool sharing of Eschweilera ovata in five forest remnants in southern Bahia, Brazil using nuclear simple sequence repeat (nSSR) and plastid simple sequence repeat (cpSSR) microsatellite markers. cpSSR marker analysis revealed the domains of four haplotypes, showing that 80% of the individuals had only four maternal origins, reflecting a founder effect and/or genetic bottleneck. The results of cpSSR and nSSR analyses indicated moderate genetic diversity, particularly in conservation units with full protection, which showed the best parameters of all areas evaluated. Another indication of the susceptibility of these populations to forest loss and fragmentation was the strong genetic bottleneck observed. In contrast, genetic structure analyses (FST and discriminant analysis of principal components) revealed gene pool sharing between the subpopulations, which may reflect the historical gene flow that occurred before forest fragmentation.

show abstract

Section: Discussionmentioning

confidence: 98%

Gene pool sharing and genetic bottleneck effects in subpopulations of Eschweilera ovata (Cambess.) Mart. ex Miers (Lecythidaceae) in the Atlantic Forest of southern Bahia, Brazil

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Genomic sequencing has revolutionised discovery of such markers (Zalapa et al 2012), whether speciesspecific (Abdelkrim et al 2009) or for use across a broader taxonomic group (Hodel et al 2016), including for several legumes (e.g. Borges et al 2015;Morris et al 2016). For example, microsatellite markers developed from the Lotus japonicus (Regel) K.Larsen genome (Sato et al 2008) were used through cross-species amplification in Lotus sessilifolius DC., a species endemic to Macaronesia (Yang et al 2018).…”

Section: Conservation Biology and Geneticsmentioning

confidence: 99%

Advances in legume research in the genomics era

Egan

Vatanparast

2019

Aust. Syst. Bot.

View full text Add to dashboard Cite

Next-generation sequencing (NGS) technologies and applications have enabled numerous critical advances in legume biology, from marker discovery to whole-genome sequencing, and will provide many new avenues for legume research in the future. The past 6 years in particular have seen revolutionary advances in legume science because of the use of high-throughput sequencing, including the development of numerous types of markers and data useful for evolutionary studies above and below the species level that have enabled resolution of relationships that were previously unattainable. Such resolution, in turn, affords opportunities for hypothesis testing and inference to improve our understanding of legume biodiversity and the patterns and processes that have created one of the most diverse plant families on earth. In addition, the genomics era has seen significant advances in our understanding of the ecology of legumes, including their role as nitrogen fixers in global ecosystems. The accumulation of genetic and genomic data in the form of sequenced genomes and gene-expression profiles made possible through NGS platforms has also vastly affected plant-breeding and conservation efforts. Here, we summarise the knowledge gains enabled by NGS methods in legume biology from the perspectives of evolution, ecology, and development of genetic and genomic resources.

show abstract

“…Despite forming crucial areas of endemism, the Atlantic Forest fragments of southern Bahia have suffered long periods of anthropogenic disturbances of the biota. For example, [ 27 ] discussed the impact of human activities on species diversity of Dalbergia nigra (Vell.) Allemao ex Benth.…”

Section: Introductionmentioning

confidence: 99%

“…Despite forming an important area of endemism, the biota of Atlantic Forest remnants in southern Bahia has suffered the incidence of long periods of anthropogenic disturbances, mainly related to deforestation. Consequently, the populations of tree species such as brauna ( Melanoxylon brauna ), Bahia rosewood ( D. nigra ), and brazilwood ( Paubrasilia echinata ) have a declined [ 11 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Gene Flow and Genetic Structure Reveal Reduced Diversity between Generations of a Tropical Tree, Manilkara multifida Penn., in Atlantic Forest Fragments

Waqar

Moraes

Benchimol

et al. 2021

Genes

Self Cite

View full text Add to dashboard Cite

The Atlantic Forest remnants in southern Bahia, Brazil, contain large tree species that have suffered disturbances in recent decades. Anthropogenic activities have led to a decrease in the population of many tree species and a loss of alleles that can maintain the evolutionary fitness of their populations. This study assessed patterns of genetic diversity, spatial genetic structure, and genetic structure among Manilkara multifida Penn. populations, comparing the genetic parameters of adult and juvenile trees. In particular, we collected leaves from adults and juveniles of M. multifida in two protected areas, the Veracel Station (EVC) and the Una Biological Reserve (UBR), located in threatened Atlantic Forest fragments. We observed a substantial decay in genetic variability between generations in both areas i.e., adults’ HO values were higher (EVC = 0.720, UBR = 0.736) than juveniles’ (EVC = 0.463 and UBR = 0.560). Both juveniles and adults showed genetic structure between the two areas (θ = 0.017 for adults and θ = 0.109 for juveniles). Additionally, forest fragments indicated an unexpectedly short gene flow. Our results, therefore, highlight the pervasive effects of historical deforestation and other human disturbances on the genetic diversity of M. multifida populations within a key conservation region of the Atlantic Forest biodiversity hotspot.

show abstract

Development of microsatellite primers for Melanoxylon brauna (Fabaceae): an endangered and endemic tree from the Brazilian Atlantic Forest

Cited by 4 publications

References 3 publications

Gene pool sharing and genetic bottleneck effects in subpopulations of Eschweilera ovata (Cambess.) Mart. ex Miers (Lecythidaceae) in the Atlantic Forest of southern Bahia, Brazil

Gene pool sharing and genetic bottleneck effects in subpopulations of Eschweilera ovata (Cambess.) Mart. ex Miers (Lecythidaceae) in the Atlantic Forest of southern Bahia, Brazil

Advances in legume research in the genomics era

Gene Flow and Genetic Structure Reveal Reduced Diversity between Generations of a Tropical Tree, Manilkara multifida Penn., in Atlantic Forest Fragments

Contact Info

Product

Resources

About