Development of measles virus in vitro

Nakai, Toshiko; Shand, F. L.; Howatson, A.F.

doi:10.1016/0042-6822(69)90127-5

Cited by 124 publications

(93 citation statements)

References 22 publications

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“…In contrast, the whole monolayer appeared to be covered with syncytia after three days and viral budding could be illustrated (Dubois-Dalcq and Reese, 1975). These observations are in agreement with previous data published for MV growth curves for the Edmonston strain in other cell lines (Black, 1959;Nakai et al, 1969). Scott and Choppin (1982) compared the virus production between the Edmonston and the Hallé strains.…”

Section: Other Measles Virus Strainssupporting

confidence: 81%

“…Higher extracellular yields appeared with increasing cell-associated virus titers, also suggesting a continuous budding of virus particles (Nakai et al, 1969). Interestingly, HeLa cells showed temperature dependant virus release.…”

Section: Extracellular and Cell-associated Virusmentioning

confidence: 94%

“…In KB cells, a maximum titer of 5.6×10 6 TCID 50 mL −1 was obtained (Kohn and Yassky, 1962). A maximum value of 1×10 7 pfu mL −1 was produced with BSC-1 cells (Nakai et al, 1969), whereas MV produced in Vero cells had a maximum titer of 1.7×10 8 TCID 50 mL −1 (Mendonca et al, 1994;. MV production in the suspension cell line U937 resulted in a titer of 6×10 6 pfu mL −1 (Brandriss et al, 1982).…”

Section: The Edmonston Strainmentioning

confidence: 99%

“…In another study by Nakai et al (1969) the yields of released virus and cell-associated virus were always within the same range (maximum titers of approximately 5×10 6 pfu mL −1 ). Higher extracellular yields appeared with increasing cell-associated virus titers, also suggesting a continuous budding of virus particles (Nakai et al, 1969).…”

Section: Extracellular and Cell-associated Virusmentioning

confidence: 99%

“…The maximum virus yield obtained with the Edmonston strain has been determined by different groups, while various cell lines were infected with this MV strain (Black, 1959;Kohn and Yassky, 1962;Nakai et al, 1969;Romano and Scarlata, 1973;Baczko and Lazzarini, 1979;Brandriss et al, 1982;Scott and Choppin, 1982;Mendonca et al, 1994; (Table 2).…”

Section: The Edmonston Strainmentioning

confidence: 99%

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Key Parameters of Measles Virus Production for Oncolytic Virotherapy

Weiss

Salzig

Mühlebach³

et al. 2012

American Journal of Biochemistry and Biotechnology

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Attenuated measles virus has revealed selective tumor cell killing and is currently tested in clinical trials for the therapy of cancer patients. The amount of infectious particles per dose needed for oncolytic therapy can be more than a million times higher compared to vaccination. This requires highly effective production processes which guarantee the measles virus quantities needed for its use in cancer therapy. Referring to measles virus production itself, several factors are influencing process parameters and subsequent virus yields. These factors are medium optimization, feeding of nutrients, an optimal multiplicity of infection, localization of produced virus particles, temperature sensitivity and time of harvest. This review summarizes the available data concerning measles virus production in cell culture and factors influencing the virus yield.

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Section: Other Measles Virus Strainssupporting

confidence: 81%

Section: Extracellular and Cell-associated Virusmentioning

confidence: 94%

Section: The Edmonston Strainmentioning

confidence: 99%

Section: Extracellular and Cell-associated Virusmentioning

confidence: 99%

Section: The Edmonston Strainmentioning

confidence: 99%

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Key Parameters of Measles Virus Production for Oncolytic Virotherapy

Weiss

Salzig

Mühlebach³

et al. 2012

American Journal of Biochemistry and Biotechnology

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Lymph node ultrastructure in hand-Schuller-Christian disease

Imamura

Muroya²

1971

Cancer

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A lymph node from a child with Hand-Schuller-Christian disease was studied by electron microscopy. I t showed a larger infiltration of two types of cells in addition to ordinary reticulum cells (littoral cells)-Langerhans' granulecontaining histiocytes a n d undifferentiated reticulum cells. Since the former invariably had a fixed ultrastructure with indented nucleus, multivesicular bodies, and peculiar f a t droplets in addition t o the Langerhans' granules, they were thought to be a type o f mesenchymal cell which might have some functions related to fat metabolism. T h e latter was characterized by the occasional presence of a strange intertwined tubular structure in the cytoplasm somewhat resembling profiles of paramyxovirus or measles virus. I n this report, the authors show the intimate relationship between these two types of cells a n d Hand-Schuller-Christian disease.

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Intracellular branched tubular structures in osteosarcoma.An ultrastructural and serological study

Jenson

Spjut

Smith

et al. 1971

Cancer

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Intracytoplasmic tubular structures, approximately 23 mμ, in diameter, were seen by electron microscopy within endothelial cells, malignant osteoblasts, and lymphocytes of an osteosarcoma. Although similar tubular structures have been seen in many different cells in vivo and in vitro, their presence within endothelial cells of glomeruli in patients with systemic lupus erythematosus prompted the suggestion that they represent measles virus nucleocapsids. Although the morphogenesis of these tubules remains obscure, we have presented evidence that these tubules may form by condensation of intracisternal granular material secreted by the endoplasmic reticulum and probably represent response of the cell to injury by providing it with more structural stability. Measles antibody titers were performed on the sera of the patient and his immediate family, because similar tubules had been equated with measles virus nucleocapsids. Because these antibody titers were all markedly elevated, a comparative ultrastructural study was undertaken between the tubules reported herein and measles virus nucleocapsids. Although the ultrastructural features were superficially similar, their morphology and morphogenesis were different. Thus, we concluded that such tubular structures could not be equated with measles virus nucleocapsids. Several possible reasons for the high measles antibody titer in the sera of the patient and his immediate family are discussed.

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Development of measles virus in vitro

Cited by 124 publications

References 22 publications

Key Parameters of Measles Virus Production for Oncolytic Virotherapy

Key Parameters of Measles Virus Production for Oncolytic Virotherapy

Lymph node ultrastructure in hand-Schuller-Christian disease

Intracellular branched tubular structures in osteosarcoma.An ultrastructural and serological study

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