Development of low cost micropropagation protocol for Nardostachys jatamansi: A critically endangered medicinal herb of Himalayas

Dhiman, Nisha; Devi, Kiran; Bhattacharya, Amita

doi:10.1016/j.sajb.2021.04.002

Cited by 17 publications

(3 citation statements)

References 48 publications

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“…Shoot cultures of N. jatamansi were obtained from the Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, Palampur, India from the previous researchers and maintained in a previously standardized MS medium [ 16 ] with 0.75 mg/L TDZ and 0.5 mg/L kinetin under in vitro condition [ 5 ]. Established shoots of 0.5–1 cm height were sub-cultured on previously defined rooting medium containing half strength MS medium, 30 g/L sucrose, 8 g/L agar, and the pH was maintained at 5.75 ± 0.2.…”

Section: Methodsmentioning

confidence: 99%

“…These secondary metabolites are used as curative agents and natural ingredients by the cosmetic, nutraceutical, and pharmaceutical industries. The immense commercial value of this species is because of its sedative, neuroprotective, hepatoprotective, antidiabetic, cardioprotective, anti-lipid peroxidative, hypolipidemic, antioxidant, tranquilizing, hypotensive, anti-inflammatory, antidepressant, anticonvulsant, antiasthmatic, antiestrogenic, and anticancer properties [ 4 , 5 ]. Thus, to improve its propagation for the conservation of this valuable Himalayan plant and to reveal its medicinal potential, researchers are exploring alternative approaches.…”

Section: Introductionmentioning

confidence: 99%

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Charged Gold Nanoparticles Promote In Vitro Proliferation in Nardostachys jatamansi by Differentially Regulating Chlorophyll Content, Hormone Concentration, and Antioxidant Activity

et al. 2022

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Nardostachys jatamansi is a critically endangered medicinal plant and endemic to the Himalayas, having high commercial demand globally. The accumulation of various secondary metabolites in its shoots and roots with antioxidant potential are well-documented in traditional as well as modern medicine systems. In the present study, we first attempted to investigate the impact of citrate (−ve charge, 11.1 ± 1.9 nm) and CTAB (+ve charge, 19.5 ± 3.2 nm) coated gold nanoparticles (AuNPs) on the in vitro proliferation and antioxidant activities of N. jatamansi. Both the nanoparticles differentially affected the morphological and biochemical parameters, chlorophyll content, internal hormone concentration, and antioxidant activities in a concentration-dependent (10–100 µM) manner. Vigorous shooting was observed in half strength MS medium supplemented with IAA (1 mg/L) with 60 µM citrate-AuNPs (46.4 ± 3.7 mm) and 40 µM CTAB-AuNPs (42.2 ± 3.2 mm). Similarly, the maximum number of roots (5.00 ± 0.67 and 5.33 ± 0.58) and root length (29.9 ± 1.5 mm and 27.3 ± 4.8 mm) was reported in half-strength MS medium with IAA (1 mg/L) supplemented with 60 µM citrate-AuNPs and 40 µM CTAB-AuNPs, respectively. In addition, plants growing on MS medium supplemented with 60 µM citrate-AuNPs and 40 µM CTAB-AuNPs showed significantly enhanced photosynthetic pigments (chlorophyll a and b, carotenoids, and total chlorophyll), internal hormone concentration (GA3, IAA, and ABA), and antioxidant activities (total phenolics, flavonoids, DPPH, and SOD enzyme activity). Moreover, the transcript analysis of ANR1, ARF18, PLY9, SAUR28, GID1A, GRF1, SOD, and CAT further confirmed the role of 60 µM citrate-AuNPs and 40 µM CTAB-AuNPs in the improvement in the growth and antioxidant activities of N. jatamansi. Bearing in mind the urgent requirements of the effective conservation measures of this endangered species, the present findings suggest the elicitation of citrate-AuNPs and CTAB-AuNPs would significantly improve the potential applications of N. jatamansi in the medicinal plant-based industry.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Charged Gold Nanoparticles Promote In Vitro Proliferation in Nardostachys jatamansi by Differentially Regulating Chlorophyll Content, Hormone Concentration, and Antioxidant Activity

et al. 2022

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“…In order to create shoots and multiple shoots in plants, a combination of BAP, IBA and kinetin appears to be the best response. Next, a concentration of BAP, NAA and half-strength MS medium demonstrated the most successful rooting, after two weeks of the culture, healthy plants were collected for future research study, according to (Dhiman et al, 2021).This study can be applied to the cost-effective in vitro production and preservation of this medicinal plant that is in grave danger of extinction.…”

Section: Comparison Of Antioxidant Properties and Flavonoid Of Natura...mentioning

confidence: 99%

Comparison of Antioxidant Properties and Flavonoid of Natural and in vitro Cultivated Nardostachys jatamansi

Pant¹,

Rautela²,

Pant³

et al. 2023

ASD

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Background: India has very rich diversity of medicinal plants. Medicinal plants are thought to be a rich source of ingredients that can be used in the development of pharmaceutical or synthetic drugs. Aside from that, these plants play an important role in the development of human drug all over the world. Whether in modern or traditional medicine, medicinal plants are used to maintain health, to treat a specific condition, or both. Nardostachys is one of the most important medicinal plant having several therapeutic properties. It is threatened in its natural habitat due to over exploitation for therapeutic purposes and high demand in the traditional medicine system. Keeping these points in mind, we attempted to investigate alternative uses of in vitro grown plants in place of wild plants without disrupting plant-based therapeutics and market demand. And then compare the root extract of the Nardostachys jatamansi plant’s antioxidant and flavonoid levels under in vitro and natural growth conditions. Methods: Nardostachys jatamansi is a plant that is widely used in traditional medicine systems. Because of its wide spread use in traditional medicine, this plant is considered endemic. In our study, we compare of antioxidant quality and Flavonoid amount of Natural and in vitro propagated Nardostachys jatamansi. Firstly we cultivated in vitro plants from Nardostachys jatamansi nodal explants for comparative analysis. The methanol extract of in vitro grown and wild-type plant root extract was then prepared using the maceration method. The extract was subjected to a comparative DPPH method to determine the presence of antioxidant potential in natural and in vitro grown plants. Furthermore, the HPLC analysis was used to detect and quantify the amount of Quercetin in both natural and in vitro propagated plants. Result: When grown in vitro at a higher concentration, the roots of Nardostachys jatamansi have greater antioxidant potential than when they are grown naturally. They demonstrated antioxidant DPPH radical scavenging activity, with an IC50 value of 29.55 µg/ml for in vitro generated plants and 24.18 µg/ml for naturally grown plants. The concentration of Quercetin (mg/ml) for natural plant species is 1.95 and for in vitro propagate plant is 1.83. The HPLC analysis presents distinct peaks, with the main peaks having retention time for standard Quercetin (10.38) in the natural plant (10.34) and in vitro grown plants (10.32). In the end, natural-type species that had been produced in vitro were used to obtain the potential of micro propagated plants. The DPPH test and flavonoid were tested on the root extract of natural and in vitro plants. Both plants displayed promising antioxidant activity and an HPLC study identified the Quercetin component.

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Assessing the Genetic Stability of In Vitro Raised Plants

Rohela

Jogam

Saini

et al. 2022

Commercial Scale Tissue Culture for Horticulture and Plantation Crops

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Development of low cost micropropagation protocol for Nardostachys jatamansi: A critically endangered medicinal herb of Himalayas

Cited by 17 publications

References 48 publications

Charged Gold Nanoparticles Promote In Vitro Proliferation in Nardostachys jatamansi by Differentially Regulating Chlorophyll Content, Hormone Concentration, and Antioxidant Activity

Charged Gold Nanoparticles Promote In Vitro Proliferation in Nardostachys jatamansi by Differentially Regulating Chlorophyll Content, Hormone Concentration, and Antioxidant Activity

Comparison of Antioxidant Properties and Flavonoid of Natural and in vitro Cultivated Nardostachys jatamansi

Assessing the Genetic Stability of In Vitro Raised Plants

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