Development of human liver extracellular matrix hydrogel for three dimensional cell culture and cell transplantation

Frenguelli, Luca; Al‐Akkad, Walid; Crowley, Claire; Tiwari, Manisha; Mani, Anne; Telese, Andrea; Coppi, Paolo De; Moore, Kevin L.; Rombouts, Krista; Pinzani, Massimo; Mazza, Giuseppe

doi:10.1016/s0168-8278(17)31009-7

Cited by 3 publications

(2 citation statements)

References 377 publications

(466 reference statements)

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“…However, cell survival in the bio-printed constructs was rather inhomogeneous ranging from 2 to 60 days [92][93][94][95] . A liver specific bio-ink, recently developed employing human liver ECM, has provided improved cell viability and albumin secretion in bio-printed constructs of hepatic cell lines or primary hepatocytes when compared with constructs of the same cells in nanocellulose 96 . In addition, recent data from bioprinting organoids derived from human liver iPSCs on alginate/pluronic hydrogel blends demonstrated improved hepatic functionality and prolonged survival in vitro, compared to single cell dispersion 97 .…”

Section: B Bioengineering Technologies For Creating Liver Tissuementioning

confidence: 99%

Regenerative hepatology: In the quest for a modern prometheus?

Papatheodoridi

Mazza

Pinzani

2020

Digestive and Liver Disease

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Section: B Bioengineering Technologies For Creating Liver Tissuementioning

confidence: 99%

Regenerative hepatology: In the quest for a modern prometheus?

Papatheodoridi

Mazza

Pinzani

2020

Digestive and Liver Disease

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“…Moreover, these materials are often not tissue-specific thereby affecting the reproduction and maintenance of specific liver metabolic function [181]. To overcome this limitation, the development of human ECM hydrogel with retained ECM protein composition using lyophilized decellularized tissue should provide the most optimal physiological microenvironment for cell culture in order to promote survival and proliferation of hepatic cells [182].…”

Section: Scaffold/matrix-based 3d Culturesmentioning

confidence: 99%

Engineering in vitro models of hepatofibrogenesis

Mazza

Al‐Akkad

Rombouts

2017

Advanced Drug Delivery Reviews

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Chronic liver disease is a major cause of morbidity and mortality worldwide marked by chronic inflammation and fibrosis/scarring, resulting in end-stage liver disease and its complications. Hepatic stellate cells (HSCs) are a dominant contributor to liver fibrosis by producing excessive extracellular matrix (ECM), irrespective of the underlying disease aetiologies, and for many decades research has focused on the development of a number of anti-fibrotic strategies targeting this cell. Despite major improvements in two-dimensional systems (2D) by using a variety of cell culture models of different complexity, an efficient anti-fibrogenic therapy has yet to be developed. The development of well-defined three-dimensional (3D) in vitro models, which mimic ECM structures as found in vivo, have demonstrated the importance of cell-matrix bio-mechanics, the complex interactions between HSCs and hepatocytes and other non-parenchymal cells, and this to improve and promote liver cell-specific functions. Henceforth, refinement of these 3D in vitro models, which reproduce the liver microenvironment, will lead to new objectives and to a possible new era in the search for antifibrogenic compounds.

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