Development of experimental in vitro burn model

Fernandes, Ana Carolina Morais; França, Jerônimo Pereira de; Gaiba, Silvana; Aloise, Antônio Carlos; Oliveira, Aline Feitoza de; Moraes, Andrea Aparecida; França, Lucimar Pereira de; Ferreira, Lydia Masako

doi:10.1590/s0102-86502014001400004

Cited by 12 publications

(9 citation statements)

References 9 publications

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“…The treatment of the burn stasis area is the key to early treatment after a burn. Animal experiment is an important part of related research, but the construction of animal models has partially limited the research progress 5‐7 . Conventional burn wound models are created on the basis of the aforementioned heated brass comb, whereby the brass block is heated and placed on the dorsum of the rat for a certain period of time under a certain pressure.…”

Section: Discussionmentioning

confidence: 99%

“…The construction of animal burn wound models representing the zone of stasis is crucial for investigations on the pathophysiological changes in the zone of stasis and the development of novel treatment modalities or drugs. However, the construction of animal models has partially limited the research progress 5‐7 . The commonly used animal burn model for studying the zone of stasis is primarily constructed by placing a brass comb on the dorsum of rats under a certain temperature, pressure, and contact duration 8 .…”

Section: Introductionmentioning

confidence: 99%

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Modification and utility of a rat burn wound model

Wang

Hong

et al. 2020

Wound Repair Regeneration

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This study aimed to improve the conventional rat burn wound model and to validate its utility. In total, 60 Sprague‐Dawley rats were divided equally into the control and experimental groups. Altogether, 60 burn wound models with zones of stasis were created in each group. Gross visual assessments of the burn wounds were performed at 0, 24, and 48 hours after burn creation. The rates of necrosis in the zones of stasis were calculated, and the blood flow from the wounds was examined. Wound tissues were collected 48 hours after the burn and subjected to hematoxylin and eosin staining to determine whether the models were successfully established. The model success rates were calculated. The success rate of the burn wound models was significantly different between the control group and the experimental group (93.33% [56/60] vs 100%; P = .042). The Cronbach's alpha values and the respective correlation coefficients indicated that the stability of the zones of stasis in the models on the two sides of the spine was higher in the experimental group than in the control group. The standard deviations of the rate of necrosis, blood flow, and density of necrotic cells and apoptosis cell density, and inflammatory factor content in the zones of stasis were smaller in the experimental group than in the control group at 48 hours after model construction. This suggested that the stability of repeated procedures was higher in the experimental group than in the control group. The novel device for creating burns in animal models facilitated the effective creation of zones of stasis for rat burn wound models. Both the model success rate and stability were higher compared with the conventional model construction method. In addition, the use of the novel device can better align with the requirements of self‐controlled studies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modification and utility of a rat burn wound model

Wang

Hong

et al. 2020

Wound Repair Regeneration

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“…The copyright holder for this preprint this version posted August 18, 2020. ; https://doi.org/10.1101/2020.08. 18.254458 doi: bioRxiv preprint thermal injury [10,11]. To mimic the three-dimensional environment of cells, human keratinocytes have also been seeded onto a de-epidermized dermis and cultured up to 20 days to study the cell-matrix interaction and cell migration in burn research [12].…”

Section: Introductionmentioning

confidence: 99%

Modeling early thermal injury using an ex vivo human skin model of contact burns

Liu

Ocotl

Karim

et al. 2021

Burns

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Background: Early mechanisms underlying the progressive tissue death and the regenerative capability of burn wounds are understudied in human skin. A clinically relevant, reproducible model for human burn wound healing is needed to elucidate the early changes in the human burn wound environment. This study reports a reproducible contact burn model on human skin that explores the extent of tissue injury and healing over time, and defines the inter-individual variability in human skin to enable use in mechanistic studies on burn wound progression and healing.Methods: Using a customized burn device, contact burns of various depths were created on human skin by two operators and were evaluated for histologic depth by three raters to determine reproducibility. Early burn wound progression and wound healing were also evaluated histologically after the thermally injured human skin was cultured ex vivo for up to 14 days.Results: Burn depths were reproducibly generated on human skin in a temperature-or time-dependent manner. No significant difference in operator-created or raterdetermined depth was observed within each patient sample. However, significant interindividual variation was identified in burn depth in ten patient samples. Burn-injured ex vivo human skin placed into culture demonstrated differential progression of cell death and collagen denaturation for high and low temperature contact burns, while reepithelialization was observed in superficial burn wounds over a period of 14 days. Conclusion:This model represents an invaluable tool to evaluate the inter-individual variability in early burn wound progression and wound healing to complement current (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.

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“…Many hyperthermia experiments use in vitro cell monolayers as their model system because of the low melting temperature of collagen hydrogels. This is not an accurate representation of heat transfer through an organ or tissue because diffusion properties and cell response to stimuli differ in a 3D environment [30,41,42]. The use of ex vivo tissue addresses the spatial limitation of cell monolayers, but these models can be cost-prohibitive and may not closely resemble living human tissue if isolated from non-human tissue.…”

Section: Introductionmentioning

confidence: 99%