Development of ethyl alcohol-precipitated silk sericin/polyvinyl alcohol scaffolds for accelerated healing of full-thickness wounds

Siritienthong, Tippawan; Ratanavaraporn, Juthamas; Aramwit, Pornanong

doi:10.1016/j.ijpharm.2012.09.043

Cited by 77 publications

(67 citation statements)

References 33 publications

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“…Extracted mitochondria were induced oxidative stress with 2.0 mM hydrogen peroxide (H 2 O 2 ) (Merck, Germany) for 5 min at 25 C. Four doses of sericin, 10, 50, 75, and 100 mg/ml, were then added together with H 2 O 2 for 30 min at 25 C to compare dose-response. ROS is a well-known indicator for oxidative stress marker.…”

Section: Biochemical Studymentioning

confidence: 99%

“…The production of ROS was measured as described in Kobroob et al 21 The assay was based on fluorescent dye transformation from 2 0 ,7 0 -dichlorofluorescein diacetate (DCFDA) to DCF or non-fluorescent to highly fluorescent stages activating by ROS. Experimented mitochondria were incubated in 2 mM DCFDA at 25 C for 1 h. Highly fluorescent stage was measured at 485 nm excitation and 530 nm emission using a fluorescence microplate reader (VICTOR 3V Multi-label plate reader, PerkinElmer, USA). ROS level was calculated in terms of difference in optical density between excitation and emission stages.…”

Section: Biochemical Studymentioning

confidence: 99%

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Sericin ameliorated dysmorphic mitochondria in high-cholesterol diet/streptozotocin rat by antioxidative property

Ampawong

Isarangkul

Aramwit

2016

Exp Biol Med (Maywood)

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The present work provides new insights regarding the antioxidative effect of sericin in (i) reducing blood cholesterol, (ii) improving liver and heart mitochondrial structures, (iii) maintaining endoplasmic reticulum integrity in exocrine pancreatic glands, and (iv) inhibiting fat deposition in the liver. Electron microscopic, histopathologic, immunohistochemical, and biochemical studies were performed. All of the results demonstrate the efficacy of sericin as a candidate for development of a functional food or adjunctive therapeutic agent against non-communicable diseases such as hypercholesterolemia. AbstractSericin has been implicated in lower cholesterolemic effect due to its properties with several mechanisms. Mitochondria are one of the most important targets to be affected in high blood cholesterol and glucose conditions. The protective role of sericin on mitochondria remains doubtful. To examine this role, electron microscopic, histopathologic, immunohistochemical, and biochemical studies were performed in a high-cholesterol diet/streptozotocin rat model. The results demonstrated that sericin reduced blood cholesterol without hypoglycemic effect. Sericin alleviated dysmorphic mitochondria in heart and liver but not in kidney and also decreased peculiar endoplasmic reticulum in the exocrine pancreas. In addition, sericin decreased hepatic steatosis and preserved zymogen granule referable to the decline of reactive oxygen species production in hepatic mitochondrial extraction and down-regulation of malondialdehyde expression in the liver and exocrine pancreas however irrelevant to lipase activity. This study suggests that sericin has antioxidative property to reduce blood cholesterol by means of diminishing fat deposit in hepatocyte and improves mitochondria and endoplasmic reticulum integrities.

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Section: Biochemical Studymentioning

confidence: 99%

Section: Biochemical Studymentioning

confidence: 99%

Sericin ameliorated dysmorphic mitochondria in high-cholesterol diet/streptozotocin rat by antioxidative property

Ampawong

Isarangkul

Aramwit

2016

Exp Biol Med (Maywood)

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“…Three-dimensional scaffolds of sericin with PVA crosslinked with genepin show accelerated healing of full-thickness wounds [56,57]. Sericin/polyvinyl alcohol scaffolds using ethyl alcohol (EtOH), and a blend of carboxymethyl cellulose with sericin appear to be effective for wound healing applications [58,59]. In vitro proliferation and migration of co-cultured keratinocytes and fibroblasts on sericin Hope porous matrices have been studied [60].…”

Section: (D) Porous 3d Spongesmentioning

confidence: 99%

Silk Protein Sericin: Promising Biopolymer for Biological and Biomedical Applications

Nayak

Kundu

2016

Biomaterials From Nature for Advanced Devices and Therapies

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“…In addition, because of strong hydrophilic characteristics, Sericin films readily swollen by aqueous solutions and can even be dissolved in aqueous solutions at elevated temperatures. Therefore, blended Sericin films have been produced along with whey protein [17], glucomannan [18] and PVA [19][20][21][22][23][24][25][26][27][28][29][30][31]. Chemical crosslinking has shown to be an effective approach to enhance the mechanical, thermal and chemical stabilities of films.…”

Section: Introductionmentioning

confidence: 99%

“…Polyvinyl alcohol (PVA) is synthetic, hot water soluble, nontoxic, biodegradable polymer with good film forming property which can be easily blended with Sericin [19][20][21][22][23][24][25][26][27][28][29][30][31]. Sericin and PVA has very good miscibility in hot water solution.…”

Section: Introductionmentioning

confidence: 99%

Flexible sericin/polyvinyl alcohol/clay blend films

et al. 2015

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This paper describes the preparation and characterization of flexible crosslinked Sericin/Polyvinyl alcohol (PVA)/ Clay blend films. Sericin/PVA (1:1 w/w) blend films are prepared by using glutaraldehyde as crosslinking agent, glycerol as plasticizer, Cloisite 30B and Sodium montmorillonite as nanoclays by solution casting method. The concentrations of glutaraldehyde, glycerol and nanoclays are optimized on the basis of mechanical properties of the blend films. It is observed that the tensile strength and elastic modulus of the crosslinked flexible Sericin/PVA film is enhanced on addition of clay. The blend films are characterized for structural, morphological and thermal properties. Fourier transform infrared spectra showed that gulteraldehyde chemically crossliked with PVA and Sericin. Scanning electron micrographs and tanδ thermograms revealed that the addition of gluteraldehyde is reduced the phase separation between Sericin and PVA in the blend films. The XRD spectra showed that clay is intercalated in Sericin/PVA matrix. Clays are completely dispersed in Sericin/PVA blend solution as observed in transmission electron microscopy (TEM).

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Development of ethyl alcohol-precipitated silk sericin/polyvinyl alcohol scaffolds for accelerated healing of full-thickness wounds

Cited by 77 publications

References 33 publications

Sericin ameliorated dysmorphic mitochondria in high-cholesterol diet/streptozotocin rat by antioxidative property

Sericin ameliorated dysmorphic mitochondria in high-cholesterol diet/streptozotocin rat by antioxidative property

Silk Protein Sericin: Promising Biopolymer for Biological and Biomedical Applications

Flexible sericin/polyvinyl alcohol/clay blend films

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