Development of electrospun nanofibers that enable high loading and long-term viability of probiotics

Škrlec, Katja; Zupančič, Špela; Mihevc, Sonja Prpar; Kocbek, Petra; Kristl, Julijana; Berlec, Aleš

doi:10.1016/j.ejpb.2019.01.013

Cited by 102 publications

(78 citation statements)

References 69 publications

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“…A three-dimensional scaffold composed of nano-sized hydrophilic polymer can absorb a large amount of water. The wide specific surface area of the nanofibers helps to trap drugs and maintain swelling for the regulation of drug dissolution and diffusion from the scaffold, thereby allowing for long-term sustained drug release to occur [19,20]. In addition, the use of a nanofibrous 3D structure is a reasonable and new structure model with unique properties that can be adjusted according to a specific purpose [21,22,23,24].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Drug Release Behavior Utilizing the Swelling Characteristics of Cellulosic Nanofibers

Lee

et al. 2019

Polymers

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It is known that the behavior of a drug released from a supporting carrier is influenced by the surrounding environment and the carrier. In this study, we investigated the drug behavior of a swellable electrospun nanofibrous membrane. Nanofibrous mats with different swelling ratios were prepared by mixing cellulose acetate (CA) and polyurethane (PU). CA has excellent biocompatibility and is capable of high water uptake, while PU has excellent mechanical properties. Paclitaxel (PTX) was the drug of choice for observing drug release behavior, which was characterized by UV-spectroscopy. FE-SEM was used to confirm the morphology of the nanofibrous mats and to measure the average fiber diameters. We observed a noticeable increase in the total volume of the nanofibrous membrane when it was immersed in water. Also, the drug release behavior increased proportionally with increasing swelling rate of the composite nanofibrous mat. Biocompatibility testing of nanofiber materials was confirmed by CCK-8 assay and cell morphology was observed. Based on these results, we propose nanofibrous mats as promising candidates in wound dressing and other drug carrier applications.

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Section: Introductionmentioning

confidence: 99%

Analysis of Drug Release Behavior Utilizing the Swelling Characteristics of Cellulosic Nanofibers

Lee

et al. 2019

Polymers

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“…To date, several types of biological products have been included in nanofibers to improve their delivery in medical applications (Figure 1). Among the cells used, probiotic bacteria have been incorporated most often (Heunis et al, 2010;Akbar et al, 2018;Skrlec et al, 2019;Zupancic et al, 2019). Cyanobacteria and fungus are also incorporated and they have therapeutic, agricultural and environmental properties.…”

Section: Biological Products In Nanofibersmentioning

confidence: 99%

“…In addition, biological products can affect the properties of the nanofibers. For example, adding Lactobacillus plantarum into polyethylene oxide (PEO) solutions increases the conductivity due to the extracellular proteins and ions introduced with the probiotics (Skrlec et al, 2019). Furthermore, different concentrations of bacteria can increase the viscosity of the polymer solution (Zupancic et al, 2019).…”

Section: Properties and Polymer Composition Of Nanofibers Containingmentioning

confidence: 99%

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Electrospun Nanofibers as Carriers of Microorganisms, Stem Cells, Proteins, and Nucleic Acids in Therapeutic and Other Applications

Stojanov

Berlec

2020

Front. Bioeng. Biotechnol.

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124

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Electrospinning is a technique that uses polymer solutions and strong electric fields to produce nano-sized fibers that have wide-ranging applications. We present here an overview of the use of electrospinning to incorporate biological products into nanofibers, including microorganisms, cells, proteins, and nucleic acids. Although the conditions used during electrospinning limit the already problematic viability/stability of such biological products, their effective incorporation into nanofibers has been shown to be feasible. Synthetic polymers have been more frequently applied to make nanofibers than natural polymers. Polymer blends are commonly used to achieve favorable physical properties of nanofibers. The majority of nanofibers that contain biological product have been designed for therapeutic applications. The incorporation of these biological products into nanofibers can promote their stability or viability, and also allow their delivery to a desired tissue or organ. Other applications include plant protection in agriculture, fermentation in the food industry, biocatalytic environmental remediation, and biosensing. Live cells that have been incorporated into nanofibers include bacteria and fungi. Nanofibers have served as scaffolds for stem cells seeded on a surface, to enable their delivery and application in tissue regeneration and wound healing. Viruses incorporated into nanofibers have been used in gene delivery, as well as in therapies against bacterial infections and cancers. Proteins (hormones, growth factors, and enzymes) and nucleic acids (DNA and RNA) have been incorporated into nanofibers, mainly to treat diseases and enhance their stability. To summarize, incorporation of biological products into nanofibers has numerous advantages, such as providing protection and facilitating controlled delivery from a solid form with a large surface area. Future studies should address the challenge of transferring nanofibers with biological products into practical and industrial use.

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“…The technology generates a dried product by elongation (due to the electrostatic forces) of the liquid feed into ultra-fine (generally < 10 μm [6]) jets, resulting in a large surface area that enables near-instantaneous drying at room temperature. Over the past years, a large number of papers have been published about the application of electrospinning for the solid formulation of various biopharmaceuticals, such as enzymes, peptides, proteins (e.g., monoclonal antibodies), oligonucleotides, and probiotics [7,8,9,10,11,12,13,14,15], showing the high interest in the application of ES for biopharmaceuticals. In order for ES to be applied for industrial use, it is necessary to scale-up the technology to achieve adequate production rates and develop downstream processing steps, e.g., milling for conversion of the produced fibers into powders suitable for powder filling (oral capsules and parenteral applications) and tableting (oral dosage forms).…”

Section: Introductionmentioning

confidence: 99%

Scaled-Up Production and Tableting of Grindable Electrospun Fibers Containing a Protein-Type Drug

et al. 2019

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The aims of this work were to develop a processable, electrospun formulation of a model biopharmaceutical drug, β-galactosidase, and to demonstrate that higher production rates of biopharmaceutical-containing fibers can be achieved by using high-speed electrospinning compared to traditional electrospinning techniques. An aqueous solution of 7.6 w/w% polyvinyl alcohol, 0.6 w/w% polyethylene oxide, 9.9 w/w% mannitol, and 5.4 w/w% β-galactosidase was successfully electrospun with a 30 mL/h feeding rate, which is about 30 times higher than the feeding rate usually attained with single-needle electrospinning. According to X-ray diffraction measurements, polyvinyl alcohol, polyethylene oxide, and β-galactosidase were in an amorphous state in the fibers, whereas mannitol was crystalline (δ-polymorph). The presence of crystalline mannitol and the low water content enabled appropriate grinding of the fibrous sample without secondary drying. The ground powder was mixed with excipients commonly used during the preparation of pharmaceutical tablets and was successfully compressed into tablets. β-galactosidase remained stable during each of the processing steps (electrospinning, grinding, and tableting) and after one year of storage at room temperature in the tablets. The obtained results demonstrate that high-speed electrospinning is a viable alternative to traditional biopharmaceutical drying methods, especially for heat sensitive molecules, and tablet formulation is achievable from the electrospun material prepared this way.

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Development of electrospun nanofibers that enable high loading and long-term viability of probiotics

Cited by 102 publications

References 69 publications

Analysis of Drug Release Behavior Utilizing the Swelling Characteristics of Cellulosic Nanofibers

Analysis of Drug Release Behavior Utilizing the Swelling Characteristics of Cellulosic Nanofibers

Electrospun Nanofibers as Carriers of Microorganisms, Stem Cells, Proteins, and Nucleic Acids in Therapeutic and Other Applications

Scaled-Up Production and Tableting of Grindable Electrospun Fibers Containing a Protein-Type Drug

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