Development of Electrochemical Sensor for Homocysteine Determination as a Cerebrovascular Disease Biomarker

Zhao, Jibo

doi:10.20964/2017.09.66

Cited by 7 publications

(3 citation statements)

References 34 publications

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“…Various well-established techniques are available for measuring nitrate and nitrite, encompassing spectroscopic [18], electrochemical [19][20][21], and biosensor [22,23] methods. Spectroscopic approaches comprise reagent-based wet chemistry, such as colorimetry based on the Griess assay [24], chemiluminescence [25], fluorescence [26], and ultraviolet (UV) absorption [27].…”

Section: Introductionmentioning

confidence: 99%

Continuous Flow with Reagent Injection on an Inlaid Microfluidic Platform Applied to Nitrite Determination

Motahari,

Morgan,

Hendricks

et al. 2024

Micromachines

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A continuous flow with reagent injection method on a novel inlaid microfluidic platform for nitrite determination has been successfully developed. The significance of the high-frequency monitoring of nutrient fluctuations in marine environments is crucial for understanding our impacts on the ecosystem. Many in-situ systems face limitations in high-frequency data collection and have restricted deployment times due to high reagent consumption. The proposed microfluidic device employs automatic colorimetric absorbance spectrophotometry, using the Griess assay for nitrite determination, with minimal reagent usage. The sensor incorporates 10 solenoid valves, four syringes, two LEDs, four photodiodes, and an inlaid microfluidic technique to facilitate optical measurements of fluid volumes. In this flow system, Taylor–Aris dispersion was simulated for different injection volumes at a constant flow rate, and the results have been experimentally confirmed using red food dye injection into a carrier stream. A series of tests were conducted to determine a suitable injection frequency for the reagent. Following the initial system characterization, seven different standard concentrations ranging from 0.125 to 10 µM nitrite were run through the microfluidic device to acquire a calibration curve. Three different calibrations were performed to optimize plug length, with reagent injection volumes of 4, 20, and 50 µL. A straightforward signal processing method was implemented to mitigate the Schlieren effect caused by differences in refractive indexes between the reagent and standards. The results demonstrate that a sampling frequency of at least 10 samples per hour is achievable using this system. The obtained attenuation coefficients exhibited good agreement with the literature, while the reagent consumption was significantly reduced. The limit of detection for a 20 µL injection volume was determined to be 94 nM from the sample intake, and the limit of quantification was 312 nM. Going forward, the demonstrated system will be packaged in a submersible enclosure to facilitate in-situ colorimetric measurements in marine environments.

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Section: Introductionmentioning

confidence: 99%

Continuous Flow with Reagent Injection on an Inlaid Microfluidic Platform Applied to Nitrite Determination

Motahari,

Morgan,

Hendricks

et al. 2024

Micromachines

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“…The screen-printed electrode is versatile, simple, low-cost, easily operated, small-sized, portable, and capable of mass production. To improve the electrochemical signal, nanomaterials were applied to SPE, such as gold nanoparticles [ 23 , 24 , 25 ], carbon nanotubes [ 26 , 27 , 28 ], graphene [ 29 , 30 ], and nanocomposite materials [ 31 ]. The sensitivity of Hcy detection was noticeably improved by applying quantum dots to the assay platform [ 15 , 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

A Label-Free Electrochemical Biosensor for Homocysteine Detection Using Molecularly Imprinted Polymer and Nanocomposite-Modified Electrodes

2023

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An essential biomarker for the early detection of cardiovascular diseases is serum homocysteine (Hcy). In this study, a molecularly imprinted polymer (MIP) and nanocomposite were used to create a label-free electrochemical biosensor for reliable Hcy detection. A novel Hcy-specific MIP (Hcy-MIP) was synthesized using methacrylic acid (MAA) in the presence of trimethylolpropane trimethacrylate (TRIM). The Hcy-MIP biosensor was fabricated by overlaying the mixture of Hcy-MIP and the carbon nanotube/chitosan/ionic liquid compound (CNT/CS/IL) nanocomposite on the surface of a screen-printed carbon electrode (SPCE). It showed high sensitivity, with a linear response of 5.0 to 150 µM (R2 of 0.9753) and with a limit of detection (LOD) at 1.2 µM. It demonstrated low cross-reactivity with ascorbic acid, cysteine, and methionine. Recoveries of 91.10–95.83% were achieved when the Hcy-MIP biosensor was used for Hcy at 50–150 µM concentrations. The repeatability and reproducibility of the biosensor at the Hcy concentrations of 5.0 and 150 µM were very good, with coefficients of variation at 2.27–3.50% and 3.42–4.22%, respectively. This novel biosensor offers a new and effective method for Hcy assay compared with the chemiluminescent microparticle immunoassay at the correlation coefficient (R2) of 0.9946.

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“…One of the most applied, sensitive, functional materials is a nanomaterial that has a large specific surface area and unique photoelectric properties [ 12 ]. Some electrochemical sensors have been developed using nanomaterials and then have been applied for measuring HcySH with good results [ 13 ]. However, some performance factors, including sensitivity, selectivity and response time, still need improvement to meet the requirements of clinical laboratory medicine.…”

Section: Introductionmentioning

confidence: 99%

An Amperometric Biomedical Sensor for the Determination of Homocysteine Using Gold Nanoparticles and Acetylene Black-Dihexadecyl Phosphate-Modified Glassy Carbon Electrode

Zhu

Zhang

et al. 2023

Micromachines

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A novel nanocomposite film composed of gold nanoparticles and acetylene black–dihexadecyl phosphate was fabricated and modified on the surface of a glassy carbon electrode through a simple and controllable dropping and electropolymerization method. The nanocomposite film electrode showed a good electrocatalytic response to the oxidation of homocysteine and can work as an amperometric biomedical sensor for homocysteine. With the aid of scanning electron microscopy, energy dispersive X-ray technology and electrochemical impedance spectroscopy, the sensing interface was characterized, and the sensing mechanism was discussed. Under optimal conditions, the oxidation peak current of homocysteine was linearly increased with its concentration in the range of 3.0 µmol/L~1.0 mmol/L, and a sensitivity of 18 nA/(μmol/L) was obtained. Furthermore, the detection limit was determined as 0.6 µmol/L, and the response time was detected as 3 s. Applying the nanocomposite film electrode for monitoring the homocysteine in human blood serum, the results were satisfactory.

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Development of Electrochemical Sensor for Homocysteine Determination as a Cerebrovascular Disease Biomarker

Cited by 7 publications

References 34 publications

Continuous Flow with Reagent Injection on an Inlaid Microfluidic Platform Applied to Nitrite Determination

Continuous Flow with Reagent Injection on an Inlaid Microfluidic Platform Applied to Nitrite Determination

A Label-Free Electrochemical Biosensor for Homocysteine Detection Using Molecularly Imprinted Polymer and Nanocomposite-Modified Electrodes

An Amperometric Biomedical Sensor for the Determination of Homocysteine Using Gold Nanoparticles and Acetylene Black-Dihexadecyl Phosphate-Modified Glassy Carbon Electrode

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