Development of Electrochemical Impedance Spectroscopy Based Malaria Aptasensor Using HRP‐II as Target Biomarker

Chakma, Babina; Jain, Priyamvada; Singh, Naveen K.; Goswami, Pranab

doi:10.1002/elan.201800142

Cited by 20 publications

(5 citation statements)

References 28 publications

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“…Using an impedimetric method to study Pf HRP-II adsorption, a B4-decorated gold electrode was fabricated. By measuring the charge transfer resistance (R CT ) during B4-mediated Pf HRP-II recognition events, the resulting electrochemical aptasensor was capable of detecting at least 3.15 pM of Pf HRP-II [ 55 ].…”

Section: Aptasensors For Parasite Detectionmentioning

confidence: 99%

Aptamer-Based Technologies for Parasite Detection

Brosseau

Vallée

Mayer‐Scholl

et al. 2023

Sensors

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Centuries of scientific breakthroughs have brought us closer to understanding and managing the spread of parasitic diseases. Despite ongoing technological advancements in the detection, treatment, and control of parasitic illnesses, their effects on animal and human health remain a major concern worldwide. Aptamers are single-stranded oligonucleotides whose unique three-dimensional structures enable them to interact with high specificity and affinity to a wide range of targets. In recent decades, aptamers have emerged as attractive alternatives to antibodies as therapeutic and diagnostic agents. Due to their superior stability, reusability, and modifiability, aptamers have proven to be effective bioreceptors for the detection of toxins, contaminants, biomarkers, whole cells, pathogens, and others. As such, they have been integrated into a variety of electrochemical, fluorescence, and optical biosensors to effectively detect whole parasites and their proteins. This review offers a summary of the various types of parasite-specific aptamer-based biosensors, their general mechanisms and their performance.

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Section: Aptasensors For Parasite Detectionmentioning

confidence: 99%

Aptamer-Based Technologies for Parasite Detection

Brosseau

Vallée

Mayer‐Scholl

et al. 2023

Sensors

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“…Thiol-containing biorecognition elements (e.g., thiol-modified aptamers) can directly be attached to Au electrodes via a Au–S bond utilizing the chemisorption property. Additionally, short thiol-containing linker molecules (e.g., 3-mercaptopropionic acid, Lomant’s reagent, aromatic thiols, cystamine) are also used for linking nonthiolated aptamers (e.g., amine-functionalized aptamers). These molecules form self-assembled monolayers (SAM) on the gold electrode surface through the thiol group, whereas their other end is utilized for aptamer immobilization.…”

Section: Fabrication Of Bioelectrodesmentioning

confidence: 99%

Advances in Bioelectrode Design for Developing Electrochemical Biosensors

Kalita,

Gogoi,

Minteer

et al. 2023

ACS Meas. Sci. Au

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The critical performance factors such as selectivity, sensitivity, operational and storage stability, and response time of electrochemical biosensors are governed mainly by the function of their key component, the bioelectrode. Suitable design and fabrication strategies of the bioelectrode interface are essential for realizing the requisite performance of the biosensors for their practical utility. A multifaceted attempt to achieve this goal is visible from the vast literature exploring effective strategies for preparing, immobilizing, and stabilizing biorecognition elements on the electrode surface and efficient transduction of biochemical signals into electrical ones (i.e., current, voltage, and impedance) through the bioelectrode interface with the aid of advanced materials and techniques. The commercial success of biosensors in modern society is also increasingly influenced by their size (and hence portability), multiplexing capability, and coupling in the interface of the wireless communication technology, which facilitates quick data transfer and linked decision-making processes in real-time in different areas such as healthcare, agriculture, food, and environmental applications. Therefore, fabrication of the bioelectrode involves careful selection and control of several parameters, including biorecognition elements, electrode materials, shape and size of the electrode, detection principles, and various fabrication strategies, including microscale and printing technologies. This review discusses recent trends in bioelectrode designs and fabrications for developing electrochemical biosensors. The discussions have been delineated into the types of biorecognition elements and their immobilization strategies, signal transduction approaches, commonly used advanced materials for electrode fabrication and techniques for fabricating the bioelectrodes, and device integration with modern electronic communication technology for developing electrochemical biosensors of commercial interest.

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“…Chakma et al [41] investigated a malaria aptasensor for the detection of histidine rich protein II (HRP-II), a specific biomarker for Plasmodium falciparum strains. HRP-II is present in the serum of plasma, cerebrospinal fluid, and urine of infected patients.…”

Section: Short Linkersmentioning

confidence: 99%

Immobilization Techniques for Aptamers on Gold Electrodes for the Electrochemical Detection of Proteins: A Review

2020

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The development of reliable biosensing platforms plays a key role in the detection of proteins in clinically and environmentally derived samples for diagnostics, as well as for process monitoring in biotechnological productions. For this purpose, the biosensor has to be stable and reproducible, and highly sensitive to detect potentially extremely low concentrations and prevent the nonspecific binding of interfering compounds. In this review, we present an overview of recently published (2017–2019) immobilization techniques for aptamers on gold electrodes for the electrochemical detection of proteins. These include the direct immobilization of thiolated aptamers and the utilization of short linkers, streptavidin/biotin interaction, as well as DNA nanostructures and reduced graphene oxide as immobilization platforms. Applied strategies for signal amplification and the prevention of biofouling are additionally discussed, as they play a crucial role in the design of biosensors. While a wide variety of amplification strategies are already available, future investigations should aim to establish suitable antifouling strategies that are compatible with electrochemical measurements. The focus of our review lies on the detailed discussion of the underlying principles and the presentation of utilized chemical protocols in order to provide the reader with promising ideas and profound knowledge of the subject, as well as an update on recent discoveries and achievements.

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Development of Electrochemical Impedance Spectroscopy Based Malaria Aptasensor Using HRP‐II as Target Biomarker

Cited by 20 publications

References 28 publications

Aptamer-Based Technologies for Parasite Detection

Aptamer-Based Technologies for Parasite Detection

Advances in Bioelectrode Design for Developing Electrochemical Biosensors

Immobilization Techniques for Aptamers on Gold Electrodes for the Electrochemical Detection of Proteins: A Review

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