Development of detection methods for ruminant interleukin (IL)-4

Hope, Jayne; Kwong, L.S.; Entrican, Gary; Wattegedera, Sean; Vordermeier, H. M.; Sopp, P.; Howard, Chris

doi:10.1016/j.jim.2005.04.010

Cited by 43 publications

(22 citation statements)

References 22 publications

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“…IL-4 and IL-10 were quantified using paired antibodies (AbD Serotec) and established protocols as previously described [29,30]. Quantification was performed using recombinant ovine IL-10 or ovine IL-4 expressed in Chinese Hamster Ovary (CHO) cells using the pEE14 ® expression vector (Lonza ™ ) according to a previously published protocol [31].…”

Section: Methodsmentioning

confidence: 99%

Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products

McNeilly

Rocchi

Bartley

et al. 2013

Vet Res

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Teladorsagia circumcincta is an important pathogenic nematode of sheep. It has been demonstrated previously that stimulation of murine T lymphocytes with excretory-secretory (ES) products derived from fourth stage larvae of T. circumcincta (Tci-L4-ES) results in de novo expression of Foxp3, a transcription factor intimately involved in regulatory T cell function. In the current study, Foxp3+ T cell responses in the abomasum and the effects of Tci-L4-ES on ovine peripheral blood mononuclear cells (PBMC) following T. circumcincta infection were investigated. T. circumcincta infection resulted in a significant increase in numbers of abomasal Foxp3+ T cells, but not an increase in the proportion of T cells expressing Foxp3. Unlike in mice, Tci-L4-ES was incapable of inducing T cell Foxp3 expression but instead suppressed mitogen-induced and antigen-specific activation and proliferation of ovine PBMC in vitro. This effect was heat labile, suggesting that it is mediated by protein(s). Suppression was associated with up-regulation of interleukin-10 (IL-10) mRNA, and specific monoclonal antibody neutralisation of IL-10 resulted in a 50% reduction in suppression, indicating involvement of the IL-10 signaling pathway. Suppression was significantly reduced in PBMC isolated from T. circumcincta infected vs. helminth-naïve lambs, and this reduction in suppression was associated with an increase in Tci-L4-ES antigen-specific T cells within the PBMC. In conclusion, we have identified a mechanism by which T. circumcincta may modulate the host adaptive immune response, potentially assisting survival of the parasite within the host. However, the impact of Tci-L4-ES-mediated lymphocyte suppression during T. circumcincta infection remains to be determined.

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Section: Methodsmentioning

confidence: 99%

Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products

McNeilly

Rocchi

Bartley

et al. 2013

Vet Res

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“…A primary anti IL-4 capture antibody (6μg/ml) was used along with a secondary biotinylated anti IL-4 antibody (2μg/ml). Known quantities of recombinant bovine IL-4 (rBoIL-4) were used as standards and a standard regression curve was fitted to the data [26]. …”

Section: Methodsmentioning

confidence: 99%

Maternal and foetal immune responses of cattle following an experimental challenge with Neospora caninum at day 70 of gestation

Bartley

Wright

Maley

et al. 2012

Vet Res

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The immune responses of pregnant cattle and their foetuses were examined following inoculation on day 70 of gestation either intravenously (iv) (group 1) or subcutaneously (sc) (group 2) with live NC1 strain tachyzoites or with Vero cells (control) (group 3). Peripheral blood mononuclear cell (PBMC) responses to Neospora antigen and foetal viability were assessed throughout the experiment. Two animals from each group were sacrificed at 14, 28, 42 and 56 days post inoculation (pi). At post mortem, maternal lymph nodes, spleen and PBMC and when possible foetal spleen, thymus and PBMC samples were collected for analysis. Inoculation with NC1 (iv and sc) lead to foetal deaths in all group 1 dams (6/6) and in 3/6 group 2 dams from day 28pi; statistically significant (p ≤ 0.05) increases in cell-mediated immune (CMI) responses including antigen-specific cell proliferation and IFN-γ production as well as increased levels of IL-4, IL-10 and IL-12 were observed in challenged dams compared to the group 3 animals. Lymph node samples from the group 2 animals carrying live foetuses showed greater levels of cellular proliferation as well as significantly (p ≤ 0.05) higher levels of IFN-γ compared to the dams in group 2 carrying dead foetuses. Foetal spleen, thymus and PBMC samples demonstrated cellular proliferation as well as IFN-γ, IL-4, IL-10 and IL-12 production following mitogenic stimulation with Con A from day 14pi (day 84 gestation) onwards. This study shows that the generation of robust peripheral and local maternal CMI responses (lymphoproliferation, IFN-γ) may inhibit the vertical transmission of the parasite.

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“…For IFN-γ, a commercially available kit was used (AbD Serotec) and for IL-4 an in-house ELISA using commercially available monoclonal antibodies (mAbs) raised against bovine IL-4 (mAb CC313 and CC314 mAb clones, AbD Serotec, Kidlington, UK). The IL-4 mAbs have been shown to be species cross-reactive for sheep and the ELISA protocol followed has been described in full by Hope et al (2005). The results for each animal were expressed as the optical density ratio between stimulation with ConA or PBS and with complete medium only.…”

Section: Animals and Dietsmentioning

confidence: 99%

Total mixed ration pellets for light fattening lambs: effects on animal health

Blanco

Giráldez

Prieto

et al. 2015

Animal

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Fifty male Merino lambs (6 to 8 weeks, 14.1 kg; n = 10 per group) were used to study the effect of feeding system: barley straw in long form and concentrate pellets in separate troughs (Control), ad libitum alfalfa supplemented with concentrate in separate troughs (Alfalfa) or including various levels of ground barley straw in concentrate pellets (B05, B15 and B25 for 50, 150 and 250 g barley straw/kg), on rumen characteristics, acid-base status, blood cell counts and lymphocyte stimulation. Alfalfa lambs had the heaviest digestive tract contents, highest rumen pH values, lowest volatile fatty acid concentration, highest papillae counts and best mucosa colour and the greatest blood pCO 2 values, lowest sodium and chloride and highest potassium concentrations (P < 0.05). Including ground barley straw in the concentrate pellet or providing straw in long form separately from the concentrate reduces rumen pH and darkens ruminal mucosa as compared with alfalfa-fed lambs, thus affecting acid-base status.

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Development of detection methods for ruminant interleukin (IL)-4

Cited by 43 publications

References 22 publications

Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products

Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products

Maternal and foetal immune responses of cattle following an experimental challenge with Neospora caninum at day 70 of gestation

Total mixed ration pellets for light fattening lambs: effects on animal health

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