“…149.9 mM NaCl, 3 mM KCl, 3 mM CaCl 2 , 0.5 mM MgCl 2 , 10 mM TES, 11 mM D-glucose, 3 g/liter lactalbumin hydrosylate (11800-026; Gibco), 2.5 g/liter TC yeastolate (255772; Difco, Detroit, MI), 5% fetal bovine serum (FBS; Hyclone, Logan, UT), 100 U/ml penicillin, and 100 µg/ml streptomycin, osmolarity adjusted to 360 mOsm with mannitol. Culture medium (supplemented Leibovitz's L-15 culture medium; Lohr et al, 2002).…”