Development of calcium phosphate/sulfate biphasic cement for vital pulp therapy

Chang, Kai‐Chun; Chang, Chia-Chieh; Chen, Wei-Tang; Hsu, Chung‐King; Lin, Fa‐Hsuan; Lin, Chun‐Pin

doi:10.1016/j.dental.2014.08.368

Cited by 12 publications

(19 citation statements)

References 36 publications

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“…In all the samples in our study in the periods of 1, 3 and 6 months after treatment, a thin layer of calcified tissuedentine bridgewas observed. These follow-up intervals matched those in the studies in which hard tissue formation was observed as early as 2 weeks after the treatment 28,33 . Finally, it should be mentioned that these findings are the result of response of a healthy dental pulp, without any inflammation, and that the performance of these materials in the presence of inflamed pulp will be assessed in further studies.…”

Section: Discussionsupporting

confidence: 81%

“…The results obtained by Tziafas at al. 32 have shown that the use of some of the growth factors, especially TGF-β, stimulates odontoblast differentiation and leads to the release of endogenous growth factors contained in the organic dentine matrix, which further stimulates dentinogenesis 33,34 . The results of the study by Popović Bajić 35 have shown a highest regularity in the organization of deeper pulp layers in the zones of the thickest dentine bridges, but also a farthest deviation from the statement concerning DDP thrombocyte rich fibrin (PRF) in animals compared to other materials.…”

Section: Discussionmentioning

confidence: 99%

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The effect of hydroxyapatite and growth factors on reparative dentine formation in the therapy of injured pulp

Velickovic¹,

Živković²,

Bubalo

et al. 2021

VOJNOSANIT PREGL

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Background/Aim. The studies of hydroxyapatite (HAp) and growth factors as the materials used for direct pulp capping have produced conflicting results for both the issue of inflammmatory response and the issue of calcified bridge formation. Calcium hydroxyapatite/poly(lactide-co-glycolide) is a bioresorbable polymer with demonstrated good characteristics as the carrier for bone morphogenetic protein (BMP) necessary in bone tissue regeneration. The role of growth factors in dental tissue reparation (in both reactionary and reparative dentinogenesis) represents the new foundation and provides a different approach to dental pulp treatment. Growth factors-TGF-beta 1directly induce morphological and functional differentiation of neodontoblasts. The aim of this experimental study was to investigate the effect of calcium hydroxyapatite/poly(lactide-coglycolide) HAp/PLGA and growth factors (TGF-β1) in the formation of a calcified tissuedentine bridgeon the teeth of our experimental model. Methods. Rodent (rabbit) teeth were used as the animal model. After the trepanation of pulp space with sterile steel drills, the pulp was capped with calcium hydroxyapatite/poly(lactide-co-glycolide) HAp/PLGA (experimental group I; n=60); calcium hydroxyapatite/poly(lactide-co-glycolide) HAp/PLGA combined with TGF-β1 growth factor (experimental group II; n=60), and there was a control group of intact teeth (n=20). The experiment was performed in general anesthesia. The animals were kept alive for 1, 3 and 6 months. The extracted teeth were adequately prepared for scanning electron microscopy.Results. Scanning electron microscopy (SEM) demonstrated that the number of teeth with calcified tissue in the form of dental bridges in the HAp/PLGA+TGF-β1 group was statistically significantly greater 6 months (66.67%) than 3 months after the treatment (26.67%), at the statistical significance level of p<0.05. Conclusion. Direct pulp capping covers the artificially exposed dental pulp and makes possible the formation of a dentine bridge (a tubular structure composed of reparative dentine) in the period of 3 months.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

The effect of hydroxyapatite and growth factors on reparative dentine formation in the therapy of injured pulp

Velickovic¹,

Živković²,

Bubalo

et al. 2021

VOJNOSANIT PREGL

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“…The strongest diffraction peaks of the HACS scaffolds were found at 2θ = 26 • , 29 • and 32.5 • , thus indicating that the crystalline hydroxyapatite was present in the scaffolds. In our previous study, we demonstrated that HA-based scaffolds had peaks at 2θ = 26 • , 29 • and 32.5 • , further confirming the presence of HA in our present scaffold [24]. Furthermore, we also showed that HA-based scaffolds had high biocompatibility and that the pores that were well-interconnected were able to enhance bone and tissue regeneration [34].…”

Section: The Characterization Of Hacs Scaffoldsupporting

confidence: 87%

“…Such studies showed enhanced osteogenesis and osteoinduction capabilities, thus laying a foundation for future bone tissue engineering research [23]. On the other hand, calcium sulfate (CS) is also a type of calcium phosphate which is widely used in bone repair and remodeling due to its excellent biodegradability and setting time [24]. Recent novel studies inspired by mussels have shown that growth factors can be attached to scaffolds via a simple immersion technique [15].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Release of Vascular Endothelial Growth Factor from 3D-Printed Poly-ε-Caprolactone/Hydroxyapatite/Calcium Sulfate Scaffold with Enhanced Osteogenic Capacity

Chen

Wang

et al. 2020

Polymers

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Vascular endothelial growth factor (VEGF) is one of the most crucial growth factors and an assistant for the adjustment of bone regeneration. In this study, a 3D scaffold is fabricated using the method of fused deposition modeling. Such a fabricated method allows us to fabricate scaffolds with consistent pore sizes, which could promote cellular ingrowth into scaffolds. Therefore, we drafted a plan to accelerate bone regeneration via VEGF released from the hydroxyapatite/calcium sulfate (HACS) scaffold. Herein, HACS will gradually degrade and provide a suitable environment for cell growth and differentiation. In addition, HACS scaffolds have higher mechanical properties and drug release compared with HA scaffolds. The drug release profile of the VEGF-loaded scaffolds showed that VEGF could be loaded and released in a stable manner. Furthermore, initial results showed that VEGF-loaded scaffolds could significantly enhance the proliferation of human mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells (HUVEC). In addition, angiogenic- and osteogenic-related proteins were substantially increased in the HACS/VEGF group. Moreover, in vivo results revealed that HACS/VEGF improved the regeneration of the rabbit’s femur bone defect, and VEGF loading improved bone tissue regeneration and remineralization after implantation for 8 weeks. All these results strongly imply that the strategy of VEGF loading onto scaffolds could be a potential candidate for future bone tissue engineering.

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“…These facts may be related to the formation of an HA-like layer on the surface of the two materials, a phenomenon well known for biomaterials similar to the ones used here. 38–42…”

Section: Discussionmentioning

confidence: 99%

Putty-like bone fillers based on CaP ceramics or Biosilicate® combined with carboxymethylcellulose: Characterization, optimization, and evaluation

et al. 2017

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Calcium phosphates and bioactive glass ceramics have been considered promising biomaterials for use in surgeries. However, their moldability should be further enhanced. We here thereby report the handling, physicochemical features, and morphological characteristics of formulations consisting of carboxymethylcellulose-glycerol and hydroxyapatite-tricalcium phosphate or Biosilicate® particles. We hypothesized that combining either material with carboxymethylcellulose-glycerol would improve handling properties, retaining their bioactivity. In addition to scanning electron microscopy, cohesion, mineralization, pH, and viscoelastic properties of the novel formulations, cell culture experiments were performed to evaluate the cytotoxicity and cell proliferation. Putty-like formulations were obtained with improved cohesion and moldability. Remarkably, mineralization in simulated body fluid of hydroxyapatite-tricalcium phosphate/carboxymethylcellulose-glycerol formulations was enhanced compared to pure hydroxyapatite-tricalcium phosphate. Cell experiments showed that all formulations were noncytotoxic and that HA-TCP60 and BGC50 extracts led to an increased cell proliferation. We conclude that combining carboxymethylcellulose-glycerol with either hydroxyapatite-tricalcium phosphate or Biosilicate® allows for the generation of moldable putties, improves handling properties, and retains the ceramic bioactivity.

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Development of calcium phosphate/sulfate biphasic cement for vital pulp therapy

Cited by 12 publications

References 36 publications

The effect of hydroxyapatite and growth factors on reparative dentine formation in the therapy of injured pulp

The effect of hydroxyapatite and growth factors on reparative dentine formation in the therapy of injured pulp

Assessment of the Release of Vascular Endothelial Growth Factor from 3D-Printed Poly-ε-Caprolactone/Hydroxyapatite/Calcium Sulfate Scaffold with Enhanced Osteogenic Capacity

Putty-like bone fillers based on CaP ceramics or Biosilicate® combined with carboxymethylcellulose: Characterization, optimization, and evaluation

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