Development of C60-based labeling reagents for the determination of low-molecular-weight compounds by matrix assisted laser desorption ionization mass spectrometry (II): Determination of thiols in human serum

Qin, Zhang-Na; Ding, Jun; Yu, Quan; Qi, Changxing; Wu, Dongmei; Zhou, Ping; Feng, Yu‐Qi

doi:10.1016/j.aca.2020.01.027

Cited by 5 publications

(3 citation statements)

References 30 publications

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“…The same samples were also processed without the addition of biothiols in order to determine the real biothiol concentration. 49 The peak area ratio of m/z 291/373 or m/z 469/373 (subtracting the corresponding endogenous thiol derivatives peak area ratio to IS) was linearly related to the spiked concentration of biothiols. The resulting calibration curves and the linear parameters were demonstrated in Figure 4b,c and Table S2, respectively.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Subsequently, the calibration in real samples such as plasma and cell lysates were further investigated, which were spiked with a series of concentrations of mixed standard solutions (Cys, 1–100 μM, others, 1–50 μM). The same samples were also processed without the addition of biothiols in order to determine the real biothiol concentration . The peak area ratio of m / z 291/373 or m / z 469/373 (subtracting the corresponding endogenous thiol derivatives peak area ratio to IS) was linearly related to the spiked concentration of biothiols.…”

Section: Resultsmentioning

confidence: 99%

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Fabrication of a “Selenium Signature” Chemical Probe-Modified Paper Substrate for Simultaneous and Efficient Determination of Biothiols by Paper Spray Mass Spectrometry

et al. 2020

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Significant efforts have been made to develop robust and reliable methods for simultaneous biothiols determination in different matrices, but there still exist the problems such as easy oxidation, tedious derivatization, and difficulty in discrimination, which brings unsatisfactory results in their accuracy and fast quantification in biological samples. To overcome these problems, a simultaneous biothiols detection method combining a “selenium signature” chemical probe and paper spray mass spectrometry (PS-MS) was proposed. In the strategy, the modified-paper substrate is used to enhance the analytical performance. Chemical probe Ebselen-NH2 that has a specific response to biothiols was designed and covalently fixed on the surface of an oxidized paper substrate. By the identification of derivatized product with distinctive selenium isotope distribution and employment of the optimized PS-MS method, qualitative and quantitative analysis of five biothiols including glutathione (GSH), cysteine (Cys), cysteinylglycine (CysGly), N-acetylcysteine (Nac), and homocysteine (Hcy) were realized. Biothiols in plasma and cell lysates were measured with satisfactory results. The established method not only provides a novel protocol for simultaneous determination of biothiols, but also is helpful for understanding the biological and clinical roles played by these bioactive small molecules.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Fabrication of a “Selenium Signature” Chemical Probe-Modified Paper Substrate for Simultaneous and Efficient Determination of Biothiols by Paper Spray Mass Spectrometry

et al. 2020

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“…An effective approach to overcome this problem is the introduction of chemical derivatization, which can increase the molecular weight of the analyte. 29,30 Our group has previously proposed the C 60 derivatization strategy for the MALDI-MS detection of small molecules in complex matrix, such as amino acids and thiols in human serum and polyamines in mice feces, [31][32][33] thanks to the high molecular weight and good MALDI-MS response of C 60 derivatives. 34 The fascinating properties of C 60 derivative in MALDI-MS analysis encouraged us to explore its application for the rapid detection of other small molecules.…”

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confidence: 99%

A boronic acid–modified C₆₀ derivatization reagent for the rapid detection of 3‐monochloropropane‐1,2‐diol using matrix‐assisted laser desorption/ionization‐mass spectrometry

Qin

Ding

et al. 2021

Rapid Comm Mass Spectrometry

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Rationale: 3-Monochloropropane-1,2-diol (3-MCPD) is a well-known contaminant formed in food thermal processing, which could be found in a variety of foodstuffs.Due to its potential carcinogenicity, it was essential to quickly develop a rapid and high-throughput analytical method to monitor 3-MCPD in foodstuffs, which is described in this study.Methods: 3-MCPD was extracted from foodstuffs and then was derivatized with a boronic acid-modified C 60 (B-C 60 ) through the boronic acid-diol reaction. Microwave heating was used to accelerate the derivatization reaction. Mass spectrometry (MS) analysis was conducted using matrix-assisted laser desorption/ionization-MS (MALDI-MS). The application of the method was validated using various smoked food samples. Results:The chemical derivatization of 3-MCPD with B-C 60 enabled the addition of a C 60 -tag to 3-MCPD. High-throughput analysis of the sample within 0.5 h was realized. A good linear range from 0.02 to 1.5 μg mL À1 for 3-MCPD was obtained, with a detection limit of 0.005 μg mL À1 . The recoveries in spiked foodstuffs ranged from 85.4% to 115.1% with relative standard deviations of 2.0%-14.2%. This method was successfully applied to detect 3-MCPD in smoked foodstuffs.Conclusions: A quantitative method was developed for the detection of 3-MCPD in foodstuffs using B-C 60 derivatization combined with MALDI-MS strategy. This proposed method may serve as a potential platform for the rapid and highthroughput analysis of 3-MCPD in foodstuffs for the purpose of food safety control.

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Research progress on nanomaterial-based matrices for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis

Du,

Yuan,

Gao

et al. 2023

Journal of Chromatography A

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Development of C60-based labeling reagents for the determination of low-molecular-weight compounds by matrix assisted laser desorption ionization mass spectrometry (II): Determination of thiols in human serum

Cited by 5 publications

References 30 publications

Fabrication of a “Selenium Signature” Chemical Probe-Modified Paper Substrate for Simultaneous and Efficient Determination of Biothiols by Paper Spray Mass Spectrometry

Fabrication of a “Selenium Signature” Chemical Probe-Modified Paper Substrate for Simultaneous and Efficient Determination of Biothiols by Paper Spray Mass Spectrometry

A boronic acid–modified C₆₀ derivatization reagent for the rapid detection of 3‐monochloropropane‐1,2‐diol using matrix‐assisted laser desorption/ionization‐mass spectrometry

Research progress on nanomaterial-based matrices for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis

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Development of C60-based labeling reagents for the determination of low-molecular-weight compounds by matrix assisted laser desorption ionization mass spectrometry (II): Determination of thiols in human serum

Cited by 5 publications

References 30 publications

Fabrication of a “Selenium Signature” Chemical Probe-Modified Paper Substrate for Simultaneous and Efficient Determination of Biothiols by Paper Spray Mass Spectrometry

Fabrication of a “Selenium Signature” Chemical Probe-Modified Paper Substrate for Simultaneous and Efficient Determination of Biothiols by Paper Spray Mass Spectrometry

A boronic acid–modified C60 derivatization reagent for the rapid detection of 3‐monochloropropane‐1,2‐diol using matrix‐assisted laser desorption/ionization‐mass spectrometry

Research progress on nanomaterial-based matrices for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis

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A boronic acid–modified C₆₀ derivatization reagent for the rapid detection of 3‐monochloropropane‐1,2‐diol using matrix‐assisted laser desorption/ionization‐mass spectrometry