Development of bis-ANS-based modified fluorescence titration assay for IFIT/RNA studies

Dobieżyńska, Anna; Miszkiewicz, Joanna; Darżynkiewicz, Zbigniew; Tyras, Michal; Stankiewicz-Drogoń, Anna; Trylska, Joanna; Darżynkiewicz, Edward; Grzela, Renata

doi:10.1016/j.bbrc.2020.09.006

Cited by 3 publications

(2 citation statements)

References 32 publications

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“… The electrostatic interactions between the probes and DNA (6.2–6.7 Å) are relatively weaker than that of RNA (Figure ). Furthermore, SYN forms more hydrogen bonds with RNA than BYD , indicating that the combination of SYN and RNA is the most stable (Figure a,c) . Because of the stronger electrostatic interaction and hydrogen bond between SYN and RNA, SYN rotation is inhibited to the greatest extent when SYN is embedded in the RNA groove.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, SYN forms more hydrogen bonds with RNA than BYD, indicating that the combination of SYN and RNA is the most stable (Figure 3a,c). 44 Because of the stronger electrostatic interaction and hydrogen bond between SYN and RNA, SYN rotation is inhibited to the greatest extent when SYN is embedded in the RNA groove. On the other hand, the nonradiative processes of the probe inserted into the hydrophobic grooves were reduced due to its twisted intermolecular charge transfer (TICT) structure feature.…”

Section: Materials Andmentioning

confidence: 99%

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Lighting Up Nucleolus To Report Mitochondria Damage Using a Mitochondria-to-Nucleolus Migration Probe

Wang,

Lin,

Liu

et al. 2024

Anal. Chem.

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Visualization of the mitochondrial state is crucial for tracking cell life processes and diagnosing disease, while fluorescent probes that can accurately assess mitochondrial status are currently scarce. Herein, a fluorescent probe named "SYN" was designed and prepared, which can target mitochondria via the mitochondrial membrane potential. Upon pathology or external stimulation, SYN can be released from the mitochondria and accumulate in the nucleolus to monitor the status of mitochondria. During this process, the brightness of the nucleolus can then serve as an indicator of mitochondrial damage. SYN has demonstrated excellent photostability in live cells as well as an extremely inert fluorescence response to bioactive molecules and the physiological pH environment of live cells. Spectroscopic titration and molecular docking studies have revealed that SYN can be lit up in nucleoli due to the high viscosity of the nucleus and the strong electrostatic interaction with the phosphate backbone of RNA. This probe is expected to be an exceptional tool based on its excellent imaging properties for tracking mitochondrial state in live cells.

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Section: Resultsmentioning

confidence: 99%

Section: Materials Andmentioning

confidence: 99%

Lighting Up Nucleolus To Report Mitochondria Damage Using a Mitochondria-to-Nucleolus Migration Probe

Wang,

Lin,

Liu

et al. 2024

Anal. Chem.

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Role of Curcumin in Mediating BSA Fibrillation in a Cu <sup>2 </sup> System: A Mechanistic Insight

Yu,

Zhu,

Hao

et al. 2023

Preprint

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Transcriptome-Wide Analysis of Human Liver Reveals Age-Related Differences in the Expression of Select Functional Gene Clusters and Evidence for a PPP1R10-Governed ‘Aging Cascade’

et al. 2021

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A transcriptome-wide analysis of human liver for demonstrating differences between young and old humans has not yet been performed. However, identifying major age-related alterations in hepatic gene expression may pinpoint ontogenetic shifts with important hepatic and systemic consequences, provide novel pharmacogenetic information, offer clues to efficiently counteract symptoms of old age, and improve the overarching understanding of individual decline. Next-generation sequencing (NGS) data analyzed by the Mann–Whitney nonparametric test and Ensemble Feature Selection (EFS) bioinformatics identified 44 transcripts among 60,617 total and 19,986 protein-encoding transcripts that significantly (p = 0.0003 to 0.0464) and strikingly (EFS score > 0.3:16 transcripts; EFS score > 0.2:28 transcripts) differ between young and old livers. Most of these age-related transcripts were assigned to the categories ‘regulome’, ‘inflammaging’, ‘regeneration’, and ‘pharmacogenes’. NGS results were confirmed by quantitative real-time polymerase chain reaction. Our results have important implications for the areas of ontogeny/aging and the age-dependent increase in major liver diseases. Finally, we present a broadly substantiated and testable hypothesis on a genetically governed ‘aging cascade’, wherein PPP1R10 acts as a putative ontogenetic master regulator, prominently flanked by IGFALS and DUSP1. This transcriptome-wide analysis of human liver offers potential clues towards developing safer and improved therapeutic interventions against major liver diseases and increased insights into key mechanisms underlying aging.

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Development of bis-ANS-based modified fluorescence titration assay for IFIT/RNA studies

Cited by 3 publications

References 32 publications

Lighting Up Nucleolus To Report Mitochondria Damage Using a Mitochondria-to-Nucleolus Migration Probe

Lighting Up Nucleolus To Report Mitochondria Damage Using a Mitochondria-to-Nucleolus Migration Probe

Role of Curcumin in Mediating BSA Fibrillation in a Cu <sup>2 </sup> System: A Mechanistic Insight

Transcriptome-Wide Analysis of Human Liver Reveals Age-Related Differences in the Expression of Select Functional Gene Clusters and Evidence for a PPP1R10-Governed ‘Aging Cascade’

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