Development of .BETA.-tricalcium Phosphate/Collagen Sponge Composite for Bone Regeneration

Abstract

Synthetic biomaterials have been developed and used for bone grafting. Here, we developed a biodegradable sponge composite for bone tissue engineering by combining β-tricalcium phosphate（β -TCP）and collagen. In addition, we sought to determine the optimal β-TCP granules/collagen ratio by evaluating and bone formation in vivo. Porous β-TCP granules were mixed with atelocollagen hydrochloride solution at various ratios -0.02, 0.05, 0.1, and 0.2 g/mL. The resultant mixtures were freeze-dried and subjected to dehydrothermal treatment in vacuo. The final composites obtained were designated β -TCP/collagen sponge composites（β-TCP/CS） . Through compression testing, it was found that the stress values for β-TCP/CS（0.2 g/mL）were higher than those of the other three composites over the whole strain range. Histological evaluation at four weeks after implantation revealed that the collagen sponge had degraded and newly formed bone was present on the surface of the β-TCP granules. At 12 weeks, the β-TCP granules were completely degraded and remodeling of the lamellar bone was observed.

Effects on bone regeneration when collagen model polypeptides are combined with various sizes of alpha-tricalcium phosphate particles

“…Collagen is known to be a promising material for transplantation, owing to its diverse applications in tissue engineering and its excellent biocompatibility and biodegradability 3) . It is possible to easily form a collagen gel that can be either injected or implanted into the defect site.…”

Design of Injectable Bone Tissue Engineering Scaffold Consists of ß-Tricalcium Phosphate Beads and Alginate

“…It has been reported that -TCP has good biodegradability and osteoconductivity as a scaffold material for bone tissue engineering (Le geros, 2002;Matsuno et al, 2006). With regard to shape availability, both block and granular forms are available.…”

Cell culture in vivo by means of diffusion chamber system

“…Osteoblast-lineage cells or bone marrowderived host cells are commonly used for this purpose, and such cells are harvested, cultured in vitro, and then inoculated into scaffolds composed of poly-L-lactic acid (PLLA) 1) , poly (DL-lactic acid-co-glycolic acid) (PLGA) [2][3][4][5] , or collagen gel [6][7][8] . Scaffolds with osteogenic cells are then implanted into the host body to induce new bone formation 9,10) .…”