Development of Anodic Titania Nanotubes for Application in High Sensitivity Amperometric Glucose and Uric Acid Biosensors

Abstract

The purpose of this study was to develop novel nanoscale biosensors using titania nanotubes (TNTs) made by anodization. Titania nanotubes were produced on pure titanium sheets by anodization at room temperature. In this research, the electrolyte composition ethylene glycol 250 mL/NH4F 1.5 g/DI water 20 mL was found to produce the best titania nanotubes array films for application in amperometric biosensors. The amperometric results exhibit an excellent linearity for uric acid (UA) concentrations in the range between 2 and 14 mg/dL, with 23.3 (μA·cm−2)·(mg/dL)−1 UA sensitivity, and a correlation coefficient of 0.993. The glucose biosensor presented a good linear relationship in the lower glucose concentration range between 50 and 125 mg/dL, and the corresponding sensitivity was approximately 249.6 (μA·cm−2)·(100 mg/dL)−1 glucose, with a correlation coefficient of 0.973.

Nanotopographical Coatings Induce an Early Phenotype-Specific Response of Primary Material-Resident M1 and M2 Macrophages

“…A comparably cost-efficient method to generate oriented nanostructures on a large scale is the fabrication of nanotubular surfaces by electrochemical anodization of Ti. Nanotube (NT) arrays were already tested in biomedical applications, demonstrating that these surfaces bear potential in drug delivery, biosensing or surface-modified implants [11][12][13]. An approach to increasing the application domain for nanotube structures is to treat Ti coatings instead of bulk material [14].…”

A novel highly selective electrochemical chlorobenzene sensor based on ternary oxide RuO2/ZnO/TiO2 nanocomposites

“…Similarly, TiO 2 is another n-type semiconductor with a wide optical band gap, and it has good stability and opto-electro-chemical properties. 48 TiO 2 , due to its multiple properties, has already been tested as an excellent sensor material to sense tetracycline, 49 hydrogen, 50 glucose, 51 formaldehyde, 52 uric acid 53 and hydrogen peroxide. 54,55 Besides this, RuO 2 exhibits brilliant electronic and electro-catalytic properties.…”

The Design of Sensitive, Selective and Reproducible Biosensor With Enzyme Immobilisation For Analytical Applications

“…The uric acid biosensor is a very attractive analytical protocol to discuss since it is known that total uric acid in serum is an indicator of diseases [32][33][34][35][36]. A biosensor with immobilization of enzyme in polytyramine membrane film is used as an example for a sensitive, selective and reproducible biosensor for uric acid assay.…”