Development of an industrial medium for economical 2,3-butanediol production through co-fermentation of glucose and xylose by Klebsiella oxytoca

Ji, Xiao‐Jun; Huang, He; Du, Jun; Zhu, Jian-Guo; Ren, Lu‐Jing; Li, Shuang; Nie, Zhi-Kui

doi:10.1016/j.biortech.2009.05.036

Cited by 87 publications

(48 citation statements)

References 24 publications

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“…Further dehydration produces 1,3-butadiene, which is the building block of synthetic rubber [4]. And the high octane rating of 2,3-BD makes it a potential aviation fuel [5,6]. Besides, 2,3-BD has potential applications in the manufacture of printing inks, perfumes, fumigants, moistening and softening agents, explosives, plasticizers, foods, and pharmaceuticals [7,8].…”

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confidence: 99%

An Effective and Simplified Fed-Batch Strategy for Improved 2,3-Butanediol Production by Klebsiella oxytoca

Nie

Huang

et al. 2010

Appl Biochem Biotechnol

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Substrate concentration in 2,3-butanediol (2,3-BD) fermentation could not be controlled well in traditional feeding strategies, such as constant, impulse, and exponential feeding strategies. In the present study, fermentative 2,3-BD production by Klebsiella oxytoca was investigated under different batch and fed-batch strategies. The glucose-feedback fed-batch strategy was proved to be not effective for economical 2,3-BD production for the inability of timely feeding, leading that the bacteria reused 2,3-BD as carbon source for cell growth. Based on the phenomena that the byproducing acids caused the pH declining and the requirement of maintaining the pH at a proper level for both cell growth and 2,3-BD accumulation, an improved strategy of pH-stat fed-batch culture with glucose and sodium hydrate fed at the same time was established. Thus, the residual glucose concentration could be controlled through the adjustment of pH automatically. At last, efficient 2,3-BD production was fulfilled under this fed-batch strategy, and the highest 2,3-BD concentration, productivity, and yield were 127.9 g/l, 1.78 g/(l•h), and 0.48 g/g (2,3-BD/glucose), respectively, compared to 98.5 g/l, 1.37 g/(l•h), and 0.43 g/g obtained in glucose-feedback fed-batch strategy. This feeding strategy was simple and easy to operate and could be feasible for industrial 2,3-BD production in the future.

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confidence: 99%

An Effective and Simplified Fed-Batch Strategy for Improved 2,3-Butanediol Production by Klebsiella oxytoca

Nie

Huang

et al. 2010

Appl Biochem Biotechnol

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“…The carbon source (glycerol) had the least effect on enzyme production. This phenomenon has been observed in many optimization studies, but there have been no definitive explanations for it [28,29]. Monitoring of the glycerol concentration and DO revealed that glycerol was not consumed when yeast extract was present in the medium (Fig.…”

Section: Medium Redesignmentioning

confidence: 85%

Improved production of Pseudomonas sp. ECU1011 acetyl esterase by medium design and fed-batch fermentation

Pan

et al. 2011

Bioprocess Biosyst Eng

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We optimized culture medium and batch-fed fermentation conditions to enhance production of an acetyl esterase from Pseudomonas sp. ECU1011 (PSAE). This enzyme enantioselectively deacetylates α-acetoxyphenylacetic acid. The medium was redesigned by single-factor and statistical optimization. The addition of ZnSO(4) enhanced enzyme production by 37%. Yeast extract concentration was directly associated with the enzyme production. The fermentation was scaled up in a 5-l fermenter with the optimized medium, and the correlations between enzyme production and dissolved oxygen, pH, and feeding strategy were investigated. The fermentation process was highly oxygen-demanding, pH sensitive and mandelic acid-inducible. The fermentation pH was controlled at 7.5 by a pH and dissolved oxygen feedback strategy. Feeding mandelic acid as both a pH regulator and an enzyme inducer increased the enzyme production by 23%. The results of the medium redesign experiments were confirmed and explained in fed-batch culture experiments. Mathematical models describing the fermentation processes indicated that the enzyme production was strongly associated with cell growth. The optimized pH and dissolved oxygen stat fed-batch process resulted high volumetric production of PSAE (4166 U/l, 7.2-fold higher than the initial) without enantioselectivity decline. This process has potential applications for industrial production of chiral mandelic acid or its derivatives.

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“…One such platform chemical of significance is 2,3-butanediol, which is used in production of plasticizers, potential aviation fuel, printing inks, perfumes, fumigants, spandex, and as a carrier for pharmaceuticals (Celinska and Grajek 2009). Various microorganisms like Serratia marcescens (Zhang et al 2010), Bacillus polymyxa (Hespell 1996;Nakashimada et al 2000), Klebsiella oxytoca (Ji et al 2009) and K. pneumoniae (Li et al 2010a) produce 2,3-butanediol of high titre from a broad spectrum of substrates. However, the main drawback for the use of these efficient 2,3-butanediol fermenting microbes is their pathogenic nature.…”

Section: Introductionmentioning

confidence: 99%

Enhanced production of 2,3-butanediol by engineered Bacillus subtilis

Biswas

Yamaoka

Nakayama

et al. 2012

Appl Microbiol Biotechnol

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Production of 2,3-butanediol by Bacillus subtilis takes place in late-log or stationary phase, depending on the expression of bdhA gene encoding acetoin reductase, which converts acetoin to 2,3-butanediol. The present work focuses on the development of a strain of B. subtilis for enhanced production of 2,3-butanediol in early log phase of growth cycle. For this, the bdhA gene was expressed under the control of P( alsSD ) promoter of AlsSD operon for acetoin fermentation which served the substrate for 2,3-butanediol production. Addition of acetic acid in the medium induced the production of 2,3-butanediol by 2-fold. Two-step aerobic-anaerobic fermentation further enhanced 2,3-butanediol production by 4-fold in comparison to the control parental strain. Thus, addition of acetic acid and low dissolved oxygen in the medium are involved in activation of bdhA gene expression from P( alsSD ) promoter in early log phase. Under the conditions tested in this work, the maximum production of 2,3-butanediol, 2.1 g/l from 10 g/l glucose, was obtained at 24 h. Furthermore, under the optimized microaerophilic condition, the production of 2,3-butanediol improved up to 6.1 g/l and overall productivity increased by 6.7-fold to 0.4 g/l h in the engineered strain compared to that in the parental control.

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Development of an industrial medium for economical 2,3-butanediol production through co-fermentation of glucose and xylose by Klebsiella oxytoca

Cited by 87 publications

References 24 publications

An Effective and Simplified Fed-Batch Strategy for Improved 2,3-Butanediol Production by Klebsiella oxytoca

An Effective and Simplified Fed-Batch Strategy for Improved 2,3-Butanediol Production by Klebsiella oxytoca

Improved production of Pseudomonas sp. ECU1011 acetyl esterase by medium design and fed-batch fermentation

Enhanced production of 2,3-butanediol by engineered Bacillus subtilis

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