Development of an in vivo mouse model to study oral submucous fibrosis

Abstract

The study provides further evidence that areca nut contributes to the development of OSF in treated animals. The model has the potential to test synergism of areca nut with other carcinogens and any therapeutic interventions.

Oral submucous fibrosis: Comparing clinical grading with duration and frequency of habit among areca nut and its products chewers

“…Table 2 -shows the association between OSMF with gutkha and arecanut. Study subjects who had gutkha only (58) were more as compared to arecanut + tobacco (28). The difference was found to be statistically significant (p=0.05).…”

“…[12][13][14][15][16][17][18][19][20][21] Chewing tobacco alone and smoking are not considered to play a role in the development of this disease, [8,11,[15][16][17] it has been conclusively proven that OSMF occurs because of areca nut consumption and is not associated with tobacco. [2][3][4]8,17,[21][22][23][24][25][26][27][28][29][30] Keeping the above points in view the present study was carried out with the aim to correlate the severity of OSMF with duration and frequency of areca nut and its product chewers.…”

Characterization of a Novel Dermal Fibrosis Model Induced by Areca Nut Extract that Mimics Oral Submucous Fibrosis

“…In that model, the buccal mucosa was treated twice daily 6 days per week with a topical application of aqueous ANE for 300–600 days. The authors of the study demonstrated that after only 600 days of treatment, the fibrotic scores for cellularity, inflammation and muscle atrophy were significantly different from those of the control group [22]. However, this model is time-consuming and requires almost the entire life span of a mouse to induce OSF.…”

“…In 2007, Sumeth Perera and coworkers demonstrated that after 300 to 600 days of ANE application twice daily to the buccal mucosa of mice, OSF development could be observed [22]. However, the establishment in one month of bleomycin-induced dermal fibrosis in mice via SC injection has been well established [23].…”

Oral submucous fibrosis: a historical perspective and a review on etiology and pathogenesis

“…The characterizations of these models were not complete and the experimental evidence was neither convincing nor reproducible. The relevance of a particular animal model to a human disease rests on its ability to parallel the biological changes that characterize the disease in humans Perera et al 45 described an OSF animal model in female albino mice of BALB/c strain. They applied an aqueous areca nut extract prepared from fresh, mature endosperms of Areca catechu…”