Development of an experimentally induced Streptococcus uberis subclinical mastitis in goats

Production and Release of Antimicrobial and Immune Defense Proteins by Mammary Epithelial Cells following Streptococcus uberis Infection of Sheep

“…This is in agreement with the observations of Lasagno et al (41). These authors reported that inoculation with S. uberis at between 8.0 ϫ 10 2 CFU and 2.4 ϫ 10 6 CFU was not sufficient to induce clinical mastitis in dairy goats; only higher loads of S. uberis (1.7 ϫ 10 8 CFU) enabled the induction of mild clinical manifestations (41). This model of infection therefore allowed us to evaluate the host immune defense in a framework of infection control and quick bacterial eradication and under a subsequent condition of clinical mastitis with bacterial survival and persistence.…”

“…On the other hand, a second inoculum of 2 ϫ 10 7 CFU led to the manifestation of clinical mastitis and pathogen detection in milk. This is in agreement with the observations of Lasagno et al (41). These authors reported that inoculation with S. uberis at between 8.0 ϫ 10 2 CFU and 2.4 ϫ 10 6 CFU was not sufficient to induce clinical mastitis in dairy goats; only higher loads of S. uberis (1.7 ϫ 10 8 CFU) enabled the induction of mild clinical manifestations (41).…”

Ultrasound and histopathological investigations of experimentally induced Staphylococcus aureus mastitis in goats

“…Data provide evidence that streptococci (S. uberis, Streptococcus agalactiae, Streptococcus dysgalactiae) induce a markedly milder inflammation of caprine udders. In support of this, Lasagno et al (2012) reported development of subclinical mastitis in goats, experimentally infected with a S. uberis isolate. The second factor contributing to acute mastitis development is the microbial dose used for intracisternal application.…”

“…The bacterial suspension used in our experiments contained 1.5 × 10 8 cfu/ml, while van Herwijnen (2010) have induced experimentally subclinical S. aureus mastitis in goats with inoculum dose of approximately 58 cfu/ml. Lasagno et al (2012) have used a S. uberis dose comparable to ours (1.7 × 10 8 cfu/ml), whereas Jing et al (2012) have successfully induced acute colimastitis in goats with application of E. coli at a dose of 3 × 10 3 cfu/ml.…”

“…In goats, clinical S. aureus mastitis was reproduced with doses of 18 × 10 3 (Rainard, 2007), 1 × 10 4 and 1 × 10 8 cfu (Ma et al, 2007). Lasagno et al (2012) have followed the course of experimentally induced subclinical mastitis in goats, inoculated with Streptococcus uberis at a dose of 1.7 × 10 8 cfu. A number of http://dx.doi.org/10.1016/j.smallrumres.2015.05.018 0921-4488/© 2015 Elsevier B.V. All rights reserved.…”